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Letter to the Editor

Poor Anti-SARS-CoV-2 Humoral and T-cell Responses After 2 Injections of mRNA Vaccine in Kidney Transplant Recipients Treated With Belatacept

Chavarot, Nathalie MD1,2; Ouedrani, Amani PhD2,3,4; Marion, Olivier MD5,6,7; Leruez-Ville, Marianne MD, PhD2,8; Vilain, Estelle BSN1; Baaziz, Maroua MD1,2; Del Bello, Arnaud MD5,6,7; Burger, Carole MD1,2; Sberro-Soussan, Rebecca MD1,2; Martinez, Frank MD1,2; Chatenoud, Lucienne MD, PhD2,3,4; Abravanel, Florence PhD5,6,9; Anglicheau, Dany MD, PhD1,2; Izopet, Jacques PhD5,6,9; Couat, Chloé NP7; Zuber, Julien MD, PhD1,2; Legendre, Christophe MD1,2; Lanternier, Fanny MD, PhD2,10; Kamar, Nassim MD, PhD5,6,7; Scemla, Anne MD1,2

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doi: 10.1097/TP.0000000000003784
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Data about coronavirus disease 2019 (COVID-19) vaccine in the general population provided an excellent profile of immunogenicity strong antibody response following the second dose of mRNA-1273 or BNT162b2 vaccines,1 associated with a potent T-cell immune response.2

Kidney transplant recipients (KTRs) exhibit lower immunogenicity to vaccines. A recent study reported a poor immune response after the first dose of mRNA anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in KTRs treated with calcineurin inhibitors.3 In abatacept (CTLA4-Ig)-treated patients, vaccination against influenza/H1N1 was associated with poor antibody response.4 This could be related to the inhibition of the crosstalk between B and T cells by the cytotoxique-T-lymphocyte-antigen 4 protein, leading to impaired germinal center formation and improper antibody response.

We aimed to assess the humoral and T-cell postvaccinal responses in 101 belatacept-treated KTRs including 54 (53.5%) at Necker Hospital (Paris, France) and 47 (46.5%) at Rangueil Hospital (Toulouse, France).

We included 101 patients (68 men [67.3%], median age: 64 y [53–73]) treated with belatacept. Median time between KT and vaccination was 59 months (29–104). Belatacept was associated with mycophenolic acid (n=79, 78.2%), mammalian target of rapamycin inhibitors (n=12, 11.9%), tacrolimus (n=8, 7.9%), azathioprine (n=2, 2.0%), and steroids (n=97, 96.0%). No patient had a history of COVID-19 infection.

Two injections of 30-μg doses of BNT162b2 mRNA COVID-19 vaccine (Pfizer-BioNTech) were administered intramuscularly 28 days apart. Vaccine and belatacept injections were performed the same day in Necker’s patients and 15 days apart in Toulouse’s patients. Antispike antibodies were assessed 28 and 60 days after the first vaccine dose using SARS-CoV-2 IgG II Quant antibody test (Abbott) in Necker and serum total SARS-CoV-2 antibodies ELISA kit (Beijing Wantai Biological Pharmacy Ent Co., Ltd, China) in Toulouse according to the manufacturer’s instructions. Both tests display a high specificity (>99%) and sensitivity (respectively 97% and 90% for WANTAI and Abbott tests).5

To analyze T-cell responses, enzyme-linked immunospot assay (EliSpot) measuring interferon-γ produced by specific SARS-CoV-2 T cells was performed at days 28 and 60. Freshly isolated peripheral blood mononuclear cells were stimulated using individual 15-mers 11-aa overlapping peptide pools derived from a peptide scan through SARS-CoV-2 spike glycoprotein (S1, S2) (JPT Peptide Technologies). Results were expressed as spot forming unit/106 CD3+ T cell. A positive response was defined by an S1 reactivity >20 spots.

Twenty-eight days after the first injection, 2 of 101 patients (2.0%) developed antispike antibodies. Among the 35 of 101 patients (34.7%) with serology testing 1 month after the second dose, 2 patients (5.7%) developed antispike antibodies (Figure 1A). The timing between vaccination and belatacept injection did not impact the seroconversion rate.

F1
FIGURE 1.:
Humoral and T-cell responses following anti-SARS-CoV-2 mRNA vaccine in KTRs treated with belatacept. A, Anti SARS-CoV-2 IgG on days 0, 28, and 60 after vaccine injection. 2.0% of patients had a positive IgG response on day 28 and 5.7% on day 60 after vaccine injection. B, Specific antispike T-cell response occurred in 2 of 40 (5%) patients on day 28 and in 7 of 23 (30.4%) patients on day 60. IgG, immunoglobulin G; KTR, kidney transplant recipient; S, spike protein; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; SFU, spot forming unit.

A specific T-cell response for the SARS-CoV-2 S1 pool of peptides was observed in 2 of 40 patients (5.0%) on day 28 and in 7 of 23 patients (30.4%) 1 month after the second injection (Figure 1B).

This is the first study assessing humoral and T-cell vaccinal responses to mRNA anti-SARS-CoV-2 vaccine in KTRs treated with belatacept. Seroconversion occurred in very few patients and T-cell response in less than one-third of patients.

The seroconversion rate at day 28 (2%) was lower than the one recently reported in a cohort of KTRs given calcineurin inhibitors (17%).3 Hence, while waiting for new strategies to improve the immunogenicity of anti-SARS-CoV-2 vaccine, such as the use of higher antigen doses or a third booster dose, or to test the efficacy of other approved vaccines, KTRs, especially those receiving belatacept, should maintain enhanced barrier measures. Vaccination of household members could also confer indirect protection to KTRs.

REFERENCES

1. Walsh EE, Frenck RW Jr, Falsey AR, et al. Safety and immunogenicity of two RNA-based COVID-19 vaccine candidates. N Engl J Med. 2020;383:2439–2450.
2. Sahin U, Muik A, Vogler I, et al. BNT162b2 induces SARS-CoV-2-neutralising antibodies and T cells in humans. medRxiv. Preprint. Posted online December 11, 2020. doi: 10.1101/2020.12.09.20245175.
3. Boyarsky BJ, Werbel WA, Avery RK, et al. Immunogenicity of a single dose of SARS-CoV-2 messenger RNA vaccine in solid organ transplant recipients. JAMA. 2021;325:1784–1786.
4. Ribeiro AC, Laurindo IM, Guedes LK, et al. Abatacept and reduced immune response to pandemic 2009 influenza A/H1N1 vaccination in patients with rheumatoid arthritis. Arthritis Care Res. 2013;65:476–480.
5. Harritshøj LH, Gybel-Brask M, Afzal S, et al. Comparison of sixteen serological SARS-CoV-2 immunoassays in sixteen clinical laboratories. J Clin Microbiol. 2021;59:e02596-20.
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