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Technique for Orthotopic Liver Transplantation in Cynomolgus Monkeys (Macaca Fascicularis)

Lee, Kyo Won1; Park, Hyojun1; Choi, Gyu-Seong1; Park, Jae Berm1; Kim, Sungjoo1; Kim, Min Jung2

doi: 10.1097/01.tp.0000520323.31680.d5
117.8
Free

1Department of Surgery, Samsung Medical Center, Seoul, Korea; 2Department of Surgery, National Police Hospital, Seoul, Korea.

Purpose: Recent studies investigating new strategies to modulate the immune system have utilized animal models of liver transplantation (LT)[1][2][3][4]. However, the anhepatic phase (AHP) remains a crucial problem in experimental LT[5][6][7]. The aim of the present study is to introduce a technique for successful orthotopic liver transplantation in cynomolgus monkeys using an early-reperfusion strategy.

Methods: Orthotopic allo-LT was performed with seven donor/recipient pairs of cynomolgus monkeys. In two recipients, liver allografts were perfused after supra-hepatic inferior vena cava (SHIVC), portal vein (PV), and infra-hepatic inferior vena cava (IHIVC) anastomosis. To reduce the time of AHP in five recipients, liver allografts ware perfused after SHIVC and PV anastomosis while the IHIVC was not anastomosed.

Results: In the latter strategy, the AHP was reduced from 46 minutes to 31 minutes and a 24-hr survival rate of 80% was achieved.

Conclusion: Our results indicate that an early-reperfusion strategy can be successfully used to establish a LT model in cynomolgus monkeys with a consistently high rate of animal survival.

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References:

1. Transplantation. 2002;73:1403-10.

2. Am J Transplant. 2012;12:1740-54.

3. Am J Transplant. 2012;12:330-40.

4. Ann Surg. 2016;263:1069-71.

5. Transplantation. 2014;98:e58-60.

6. J Vis Exp. 2015:e52055.

7. G Chir. 1990;11:206-10.

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