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Expression of lncRNA-AK005641 in Murine CD4+FOXP3+Treg and iTreg Differentiation

Cheukfai, Li; Wei, Zhang; Yifan, Xiang; Zhiyong, Guo; Weiqiang, Ju; Dongping, Wang; Xiaoshun, He

doi: 10.1097/01.tp.0000520296.28521.7c
115.4
Free

Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.

Backgound: Recent studies have shown that lncRNAs play crucial role in the innate immune response and T cell development, activation, and differentiation. However, little is known how they function in T cell subset polarization, such as regulatory T cells (Treg) and Th17. We reported lncRNAs profile in murine heart allograft rejection, provided differentially expressed lncRNAs.[1]

Methods: Naive CD4+ CD25- CD62Lhi T cells were purified from the splenocytes of B6.Cg-Foxp3tm2Tch/J (Foxp3/GFP) transgenic mice. Regulatory T cell culture was conducted as previous. lncRNAs expression was test by qPCR.

Results: Observing the various expression in lncRNA microarray results, the expression of lncRNA-AK005641 was downregulated in murine heart allograft. Next we desired to further know the relevance of lncRNAs in various lymphocyte subsets. CD4+Foxp3+ T cells, regarded as nTreg, were sorted from Foxp3/GFP transgenic mice splenocytes. The expression of lncRNA-AK005641 was significantly upregulated in nTreg(GFP+) comparing with CD4+Foxp3- T cells (GFP-). The expression of lncRNA-AK005641 was significantly upregulated in iTreg comparing with CD4+Foxp3- T cells. Subsequently, iTreg and Th0 polarization condition culture were performed. In the period from naïve T cell differentiated into iTreg, the expression of lncRNA-AK005641 was upregulated when compared with Th0 culture in each time point. On day 3, the CD4+ Foxp3+ T cells(GFP+) and CD4+ Foxp3- (GFP-)were sorted from iTreg culture. The expression of AK005641 was significantly upregulated in iTreg. Taken together, the result indicated that lncRNA-AK005641 had a high expression in both nTreg and iTreg.

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Conclusions: Our results provide a preliminary profile of lncRNA–AK005641 that may regulate immune response, especially in Tregs differentiation. We will further analyze the expression of lncRNA–AK005641 in transplant recipients’ PBMC, owing to test the relevance between lncRNA–AK005641 and clinical rejection or tolerance.

References:

1. Gu G, et al. Transplantation. 2017 Jan;101(1):83–91.

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