Institutional members access full text with Ovid®

Share this article on:

The Anti-inflammatory Effect of Hydrogen on Lung Transplantation Model of Pulmonary Microvascular Endothelial Cells During Cold Storage Period

Zhang, Guangchao, MD1; Li, Zhe, MD1; Meng, Chao, PhD1; Kang, Jiyu, MD1; Zhang, Mengdi, MD1; Ma, Liangjuan, PhD2; Zhou, Huacheng, PhD1

doi: 10.1097/TP.0000000000002276
Original Basic Science—General

Background Lung ischemia-reperfusion injury (LIRI) remains an important factor for the early mortality of lung transplantations. Hydrogen (H2) can attenuate lung injury and improve lung function in animal experiments. In previous studies, pulmonary microvascular endothelial cells (PMVECs) were used to simulate LIRI. We hypothesized that H2 can alleviate inflammatory injury in a PMVECs lung transplantation model in the cold ischemia phase.

Methods Pulmonary microvascular endothelial cells were divided into 4 groups: blank, control, oxygen (O2), and H2. The blank group included PMVECs without treatment. During the cold storage period, the O2 group was aerated with 40% O2 and 60% N2, and the H2 group was aerated with 3% H2, 40% O2, and 57% N2. The control group was aerated without gases. The mixed gases were replaced every 20 minutes for 4 hours. During the transplantation period, the sealed containers were warmed for 1 hour at room temperature. In the reperfusion period, the containers were aerated with 50% O2, 5% CO2 and 45% N2 at 37°C.

Results The concentrations of interleukin-6 and tumor necrosis factor-α in the extracellular solutions were significantly decreased, and the concentration of interleukin-10 was increased in the H2 group. Intercellular adhesion molecule-1 expression was inhibited by H2. Furthermore, H2 decreased the activation of NF-κB and phosphorylation level of p38. Cell apoptosis was alleviated. The pathological changes in the cell and mitochondria were alleviated after H2 administration.

Conclusions Hydrogen-attenuated inflammatory response in a PMVECs lung transplantation model during cold storage. The effect may be achieved by inhibition of p38 MAPK and NF-κB pathways.

The authors demonstrate that 3% hydrogen and 40% oxygen administration during the cold storage period improves the hypoxic injury and attenuates the inflammatory response in a simulated lung transplantation model using PMVECs.

1 Department of Anesthesiology, the Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.

2 Department of Dermatology, the Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, China.

Received 28 November 2017. Revision received 29 April 2018.

Accepted 1 May 2018.

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The National Nature Science Foundation of China (grant 81570088).

The authors declare no conflicts of interest.

G.Z. conceived and designed the research, analyzed the data, performed statistical analysis, handled funding and supervision, drafted the article, and made critical revision of the article for important intellectual content. Z.L. conceived and designed the research, acquired the data, performed statistical analysis, drafted the article, and made critical revision of the article for important intellectual content. C.M. conceived and designed the research, acquired the data, and made critical revision of the article for important intellectual content. J.K. conceived and designed the research, acquired the data, made critical revision of the article for important intellectual content. M.Z. acquired the data, analyzed the data, made critical revision of the article for important intellectual content. L.M. analyzed the data, made critical revision of the article for important intellectual content. H.Z. conceived and designed the research, analyzed the data, performed statistical analysis, and made critical revision of the article for important intellectual content.

Correspondence: Huacheng Zhou, PhD, Department of Anesthesiology, the Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China. (zhouhuacheng@163.com).

Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.