THE INCIDENCE OF ANTIMONOCYTE ALLOANTIBODIES: PDF OnlyPREVENTION OF THE INDUCTION OF ALLOSPECIFIC CYTOTOXIC T LYMPHOCYTE AND DELAYED-TYPE HYPERSENSITIVITY RESPONSES BY ULTRAVIOLET IRRADIATION OF CORNEAL ALLOGRAFTSNIEDERKORN, JERRY Y.; CALLANAN, DAVID; ROSS, JOEL R. Author Information Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 Transplantation: August 1990 - Volume 50 - Issue 2 - p 281-285 Free Abstract The effect of ultraviolet radiation (UVR) on the immunogenicity of corneal allografts was examined in a mouae model. Corneal allografts differing from the host at the entire MHC and multiple minor H loci were subjected to 200 mJ/cm3 of UVB irradiation immediately prior to heterotopic transplantation. Analysis of cytotoxic T lymphocyte and delayed-type hypersensitivity responses revealed that UVR treated corneal grafts failed to induce either CTL or DTH responses in C57BL/6 recipients. UVB treatment abolished the immunogenicity of highly immunogenic corneal grafts containing either resident or infiltrating donor-specific Langerhans cells. Sequential grafting experiments demonstrated that UVB-treated grafts rendered the hosts anergic to subsequent immunization with highly immunogenic corneal limbus grafts that contained dense concentrations of Ia+ Langerhans cells of donor origin. The results indicate that UV treatment not only reduces the immunogenicity of the corneal allograft but may also render it tolerogenic. © Williams & Wilkins 1990. All Rights Reserved.
