ARTICLES: PDF OnlySPONTANEOUS ANTI-TUBULAR-BASEMENT-MEMBRANE ANTIBODY PRODUCTION BY LYMPHOCYTES ISOLATED FROM A REJECTED ALLOGRAFTJORDAN, S. C.2,3; BARKLEY, S. C.; LEMIRE, J. M.; SAKAI, R. S.; COHEN, A.4; FINE, R. N.Author Information Division of Pediatric Nephrology, Department of Pediatrics, University of California, Los Angeles, School of Medicine; and Department of Pathology, Harbor-UCLa School of Medicine, Torrance, California 2Dr. Jordan is the recipient of a Research Career Development Award (1-KO4-AM 01183) from the National Institute of Arthritis, Diabetes and Digestive and Kidney Diseases. 4Harbor-UCLA School of Medicine Transplantation: February 1986 - Volume 41 - Issue 2 - p 173-176 Free Abstract The immunological events mediated by, and antigen specificity of, allograft-bound lymphocytes (ABLs) are poorly understood. To further define the role of anti-body-mediated rejection, a rejected allograft from a patient with primary anti-TBM disease was sterilely minced and pressed through a microscreen. The ABLs were isolated by density gradient centrifugation. Using this technique, 8.5x106 ABLs were isolated. Then 1x106 washed ABLs/ml were suspended in RPMI 1640 with 20% fetal calf serum and cultured in microtiter plates with media only, or with pokeweed mitogen plates with media only, or with pokeweed mitogen (PWM) (100 $mUg/culture). The cells were incubated for 7 days and supernatants were collected and assayed for total IgG and IgM by a solid-phase enzyme immunoassay (EIA) and reactivity with normal human kidney targets by indirect immunofluorescence (IF) and immunoperoxidase (IP) techniques. Total IgG production was 500 ng/ml for both spontaneous and PWM stimulated cells. No IgM production was detected. IF and IP studies demonstrated IgG-anti-TBM antibodies in the spontaneous supernatants only. IgG antibodies reactive with peritubular capillaries (anti-PTC) were also noted. IgG-anti-TBM anti-bodies and antibodies reactive with arterioles were subsequently demonstrated by direct immunofluorescence techniques in the rejected allograft. Analysis of serum samples obtained at the time of allograft rejection showed no IF or IP reactivity with the Kidney targets. Subsequent analysis of anti-TBM production by the patient's peripheral blood mononuclear cells (PBMs) showed IgM-anti-TBM only. These studies suggest that the IgG-anti-TBM and IgG-anti-PTC antibodies reactive with the allograft resulted from in situ antibody production by ABLs; the role of anti-TBM antibodies in mediating the AR is unclear, but their presence suggests recurrence of the original disease in the allograft. Anti-PTC antibodies could be important in mediation of the vascular AR. © Williams & Wilkins 1986. All Rights Reserved.