ORIGINAL ARTICLE: PDF OnlyRubinstein PabloTransplantation: November 1964 - p 695-706 Free Abstract Anti-H-2 antibody responses against spleen and liver tissue were investigated. Some of the different antibodies were measured separately. The results show that spleen is a much better H-2 antibody inducer than liver in the two strain combinations studied. This is rather in agreement with the evidence in other test systems and shows that a given tissue's paucity in the induction of immunologic phenomena does not necessarily correlate with an absence of antigens. The H-2 antigens determined by the H-2S allele were shown to be separable by homogenization of spleen tissue and centrifugation at 11000 g for 1 hour. While the E antigen was found both in supernatant and sediment, under these circumstances, the S antigen was found only in the sediment. Several of the antibody titer curves were found to be bi- or tri-modal. The bearing of these findings on the genetics and chemistry of the H-2 antigens, and the passive transfer of transplantation immunity is discussed. © Williams & Wilkins 1964. All Rights Reserved.