The purpose of this study was to track and investigate the effects of autologous bone marrow-derived mesenchymal stem cells (MSCs) transplantation after acute myocardial infarction in swine assessed by magnetic resonance imaging (MRI).
Twenty-four Chinese mini-pigs (27±3 kg) were divided into 4 groups, including control groups (groups 1 and 3) and MSCs transplantation groups (group 2, super paramagnetic iron oxide labeled and group 4, 4′,6-diamidino-2-phenylindole labeled). Super paramagnetic iron oxide-labeled and 4′,6-diamidino-2-phenylindole-labeled MSCs (3.0×106 cells/mL) with a volume of 10 mL were injected into the left anterior descending artery by a catheter at 1 week after acute myocardial infarction, respectively. Cell distribution, cardiac functions, and scar tissue were quantitatively assessed by MRI.
The reduction of the T2* value in the myocardium, spleen, and liver in group 2 was significantly greater than that in group 1. MRI showed that function and scar size at baseline and 3 days after cell infusion were not significantly different between groups 1 and 2. Six weeks later left ventricular ejection fraction (P<0.0001), end-systolic volume (P<0.05), the number of dyskinetic segments (P<0.0001), left ventricular weight index (P<0.0001), and the infarcted size (P<0.0001) in group 4 were all improved comparing with those in group 3.
The majority of MSCs entrapped by the extracardial organs were mainly in the spleen. Catheter-based delivery of autologous bone marrow-derived MSCs into infarcted myocardium is feasible and effective.
Supplemental Digital Content is available in the text.
Departments of *Radiology
§Pathology and Histology, Fuwai Hospital, Peking Union Medical College, People's Republic of China
The authors declare no conflicts of interest.
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Supported by a research grant of National Natural Science Foundation of China (30471722 & 81000604) and Peking Union Medical College Research Fund for Youth (2009-xhj4♯).
Reprints and corresponding author: Shihua Zhao, MD, Department of Radiology, Fuwai Hospital, Peking Union Medical College (PUMC) and Chinese Academy of Medical Sciences (CAMS), Beijing, 100037, People's Republic of China (e-mail: email@example.com).