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The Frequency of Discordant Gyrase A Genotypes Among Cases of Multiple Neisseria gonorrhoeae Infections at Different Anatomic Sites

Allan-Blitz, Lao-Tzu MD*; Ellis, Olivia L. MS, MPH†‡; Bolan, Robert MD§; Hemarajata, Peera MD, PhD; Humphries, Romney M. PhD‡¶; Shahkolahi, Akbar MD; McGrath, Mark MD**; Klausner, Jeffrey D. MD, MPH††‡‡

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Sexually Transmitted Diseases: January 2019 - Volume 46 - Issue 1 - p e3-e4
doi: 10.1097/OLQ.0000000000000912
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The emergence of multidrug-resistant Neisseria gonorrhoeae infections has caused great concern,1,2 and has resulted in novel approaches to combat antimicrobial resistance.3 One approach uses antibiotics which are no longer recommended for empiric therapy for N. gonorrhoeae. That has been made possible by rapid molecular genotypic assays for predicting antimicrobial susceptibility.4 The gyrase A (gyrA) assay is a real-time molecular test which determines the presence of mutation at codon 91 of the gyrA gene5; the absence of mutation at this locus has been shown to reliably predict susceptibility to ciprofloxacin.6 The use of ciprofloxacin as opposed to dual therapy with ceftriaxone and azithromycin affords several benefits including the reduction in selection pressure for the emergence of ceftriaxone resistance.

In November 2015, the University of California Los Angeles Health System implemented gyrA genotyping on all N. gonorrhoeae positive clinical specimens.7 In working with the gyrA assay, clinicians have questioned, among cases in which individuals have multiple anatomic sites involved, what is the likelihood that all infections have the same gyrA genotype? Previous studies have demonstrated that mixed infections with multiple strains of N. gonorrhoeae at the same anatomic site may occur.8,9 Therefore, it is plausible that infections at different anatomic sites may also reflect different strains of N. gonorrhoeae, and therefore different antimicrobial susceptibility profiles. One study identified 3 cases of N. gonorrhoeae infections harboring strains with differing susceptibilities to ciprofloxacin at unique anatomic sites.10 Further data, however, are lacking. To test that hypothesis further, we aimed to characterize the frequency of discordant genotype results among cases that were successfully gyrA genotyped at the University of California Los Angeles Clinical Microbiology Laboratory and the Los Angeles Lesbian Gay Bisexual and Transgender Center and had more than 1 anatomic site of infection.

METHODS

We performed a retrospective chart review of all N. gonorrhoeae cases with more than 1 anatomic site of involvement that were successfully genotyped between November 1, 2015, and April 28, 2017, at the University of California Los Angeles. Specimens that were N. gonorrhoeae positive as determined by the Cobas 4800 CT/NG Assay (Roche Molecular Systems, Pleasanton, CA) were reflexed to gyrA genotyping. A single case was defined as a new diagnosis of N. gonorrhoeae infection at one or more anatomic site on a given date.

We also included N. gonorrhoeae positive specimens from participants presenting for routine sexual health screening at the Los Angeles Lesbian Gay Bisexual and Transgender Center between September 1, 2016, and February 28, 2018, tested at the Los Angeles County Public Health Laboratory (APTIMA Combo 2 CT/NG assay; Gen-Probe, San Diego, CA). Those specimens which were N. gonorrhoeae positive were also reflexed to gyrA genotyping.

GyrA genotyping was performed using a real-time polymerase chain reaction assay with high-resolution melt analysis. That, along with fluorescence resonance energy transfer probes, which target the codon 91 region of the gyrA gene, enabled us to determine wild-type (nonmutated) versus mutant gyrA sequences. The sequences of the probes used were: 5′-GCA-TCG-TCG-GCG-ACG-TCA-TCG-GTA-AAT-ACC-ACC-C-3′-fluorescein and 5′-Red 640-ACG-GCG-ATT-CCG-CAG-TT-3′-phosphorylated.

We then determined the frequency of discordant genotype results and the 95% confidence interval. All analysis was performed using STATA software version 15.0 (StataCorp; College Station, TX). The study and the analysis were approved by the University of California Los Angeles institutional review board.

RESULTS

During the study period, 1243 cases of N. gonorrhoeae infection were identified. Of those, 125 (10%) cases had greater than 1 anatomic site involved and were successfully genotyped. Four (3.2%; 95% confidence interval, 0.9%–8.0%) had discordant results (Table 1).

TABLE 1
TABLE 1:
Four Cases of Neisseria gonorrhoeae Infection Involving Multiple Anatomic Sites With Discordant gyrA Genotype Results

DISCUSSION

We determined the frequency of discordant gyrA genotype N. gonorrhoeae infections among patients with N. gonorrhoeae detected at more than 1 anatomic site, diagnosed at the University of California Los Angeles Clinical Microbiology Laboratory or at the Los Angeles Lesbian Gay Bisexual and Transgender Center. Our results provide evidence that a low proportion of anatomically separate N. gonorrhoeae infections in the same patient detected on the same date have discordant gyrA genotypes, and thus different susceptibilities to ciprofloxacin. That finding should provide reassurance in the routine introduction of gyrA testing into clinical practice and may obviate the need to perform genotyping on each anatomic site of infection in a single patient. However, should gyrA genotyping be done only from specimens collected from 1 anatomic site, there might be a small proportion of inadequately treated infections.

Our findings are in agreement with a prior study that identified a small, but notable proportion of cases of N. gonorrhoeae infections with multiple anatomic sites involved, which have different susceptibilities to ciprofloxacin and other antibiotics; that study found 3 (4.2%) cases where there were differences in ciprofloxacin susceptibility.10 Those 3 cases had infections at the rectum and urethra, while our results provided evidence that other combinations of anatomic sites may also be infected by different strains of N. gonorrhoeae.

Furthermore, our findings may provide the ground work for future studies investigating the relationship between anatomically isolated but concurrent infections and antimicrobial susceptibility. Such research may inform the transmission dynamics of resistance genes, and further inform implementation of rapid molecular assays.

Our study had several limitations. Primarily, the precision of our findings is limited by the sample size. Furthermore, given the methods of selecting cases, we were unable to determine the prevalence of discordant results. Finally, the samples were all collected in Los Angeles. Further studies should aim to include specimens from other regions. However, given our aim of characterizing the frequency of discordant results to test the hypothesis that N. gonorrhoeae from only 1 anatomic site needs to be gyrA genotyped among cases in which there are multiple anatomic sites infected, we feel that those limitations do not negate the importance of our findings.

In conclusion the frequency of discordant gyrA genotype N. gonorrhoeae infections was low. Obtaining genotype results from 1 anatomic site may be sufficient to guide antimicrobial therapy. Further studies in larger samples and different populations are needed to identify the actual prevalence of discordant genotype results.

REFERENCES

1. Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States, 2013. Current 114.
2. Ventola CL. The antibiotic resistance crisis: Part 1: Causes and threats. P T 2015; 40:277–283.
3. Wi T, Lahra MM, Ndowa F, et al. Antimicrobial resistance in Neisseria gonorrhoeae: Global surveillance and a call for international collaborative action. PLoS Med 2017; 14.
4. Low N, Unemo M. Molecular tests for the detection of antimicrobial resistant Neisseria gonorrhoeae: When, where, and how to use? Curr Opin Infect Dis 2016; 29:45–51.
5. Hemarajata P, Yang S, Soge OO, et al. Performance and verification of a real-time PCR assay targeting the gyrA Gene for prediction of ciprofloxacin resistance in Neisseria gonorrhoeae. J Clin Microbiol 2016; 54:805–808.
6. Allan-Blitz LT, Wang X, Klausner JD. Wild-type gyrase A genotype of Neisseria gonorrhoeae predicts in vitro susceptibility to ciprofloxacin: A systematic review of the literature and meta-analysis. Sex Transm Dis 2017; 44(5):261–265.
7. Allan-Blitz LT, Humphries RM, Hemarajata P, et al. Implementation of a rapid genotypic assay to promote targeted ciprofloxacin therapy of Neisseria gonorrhoeae in a large health system. Clin Infect Dis 2017; 64:1268–1270.
8. Martin IM, Ison CA. Detection of mixed infection of Neisseria gonorrhoeae. Sex Transm Infect 2003; 79(1):56–58.
9. Lynn F, Hobbs MM, Zenilman JM, Behets FM, Van Damme K, Rasamindrakotroka A, Bash MC. Genetic typing of the porin protein of Neisseria gonorrhoeae from clinical noncultured samples for strain characterization and identification of mixed gonococcal infections. J Clin Microbiol 2005; 43:368–75.
10. Pond MJ, Hall CL, Miari VF, et al. Accurate detection of Neisseria gonorrhoeae ciprofloxacin susceptibility directly from genital and extragenital clinical samples: Towards genotype-guided antimicrobial therapy. J Antimicrob Chemother 2016; 71:897–902.
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