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The Etiology of Vaginal Discharge Syndrome in Zimbabwe: Results from the Zimbabwe STI Etiology Study

Chirenje, Z., Mike, MD, FRCOG*; Dhibi, Nicholas, BS, MSc*; Handsfield, H., Hunter, MD; Gonese, Elizabeth, MPH; Tippett Barr, Beth, DrPH; Gwanzura, Lovemore, BS, PhD§¶; Latif, Ahmed, S., MBChB, MD, FAFPHM; Maseko, Dumisili, Venessa, BTech**; Kularatne, Ranmini, S., MBChB, MSc**; Tshimanga, Mufuta, MD, MPH††; Kilmarx, Peter, H., MD‡‡‡; Machiha, Anna, SRN§§; Mugurungi, Owen, MD§§; Rietmeijer, Cornelis, A., MD, PhD, MSPH††¶¶∥∥

Sexually Transmitted Diseases: June 2018 - Volume 45 - Issue 6 - p 422–428
doi: 10.1097/OLQ.0000000000000771
The Real World of STD Prevention
Free
SDC

Introduction Symptomatic vaginal discharge is a common gynecological condition managed syndromically in most developing countries. In Zimbabwe, women presenting with symptomatic vaginal discharge are treated with empirical regimens that commonly cover both sexually transmitted infections (STIs) and reproductive tract infections, typically including a combination of an intramuscular injection of kanamycin, and oral doxycycline and metronidazole regimens. This study was conducted to determine the current etiology of symptomatic vaginal discharge and assess adequacy of current syndromic management guidelines.

Methods We enrolled 200 women with symptomatic vaginal discharge presenting at 6 STI clinics in Zimbabwe. Microscopy was used to detect bacterial vaginosis and yeast infection. Nucleic acid amplifications tests were used to detect Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, and Mycoplasma genitalium. In addition, serologic testing was performed to detect human immunodeficiency virus (HIV) infection.

Results Of the 200 women, 146 (73%) had an etiology detected, including bacterial vaginosis (24.7%); N. gonorrhoeae (24.0%); yeast infection (20.7%); T. vaginalis (19.0%); C. trachomatis (14.0%) and M. genitalium (7.0%). Among women with STIs (N = 90), 62 (68.9%) had a single infection, 18 (20.0%) had a dual infection, and 10 (11.1%) had 3 infections.

Of 158 women who consented to HIV testing, 64 (40.5%) were HIV infected.

The syndromic management regimen covered 115 (57.5%) of the women in the sample who had gonorrhea, chlamydia, M. genitalium, or bacterial vaginosis, whereas 85 (42.5%) of women were treated without such diagnosis.

Conclusions Among women presenting with symptomatic vaginal discharge, bacterial vaginosis was the most common etiology, and gonorrhea was the most frequently detected STI. The current syndromic management algorithm is suboptimal for coverage of women presenting with symptomatic vaginal discharge; addition of point of care testing could compliment the effectiveness of the syndromic approach.

Among women presenting with symptomatic vaginal discharge at primary health clinics in Zimbabwe, bacterial vaginosis (24.7%) and Neisseria gonorrhoeae infection (24%) were the most common etiologies.

From the *Clinical Trial Unit, University of Zimbabwe College of Health Sciences, Belgravia, Harare, Zimbabwe;

Division of Allergy and Infectious Diseases, Department of Medicine and the Center for AIDS and STD, University of Washington School of Medicine, Seattle WA;

Division of Global Health and Tuberculosis, Centers for Disease Control and Prevention;

§Department of Medical Laboratory Sciences, College of Health Sciences, University of Zimbabwe;

Biomedical Research and Training Institute, Avondale, Harare, Zimbabwe;

Public Health Specialist, Brisbane, Australia;

**Sexually Transmitted Infections Section, Centre for HIV and STIs, National Institute for Communicable Diseases, Johannesburg, South Africa;

††Surveillance, Evaluation, Assessment and Monitoring (SEAM) Project, Department of Community Medicine, College of Health Sciences, University of Zimbabwe, Harare, Zimbabwe;

‡‡Fogarty International Center, National Institutes of Health, Bethesda, MD;

§§Zimbabwe Ministry of Health and Child Care, Harare, Zimbabwe;

¶¶Department of Behavioral and Community Health, Colorado School of Public Health, University of Colorado Denver; and

∥∥Rietmeijer Consulting, Denver, CO

Acknowledgements: ZiCHIRe Study Team: Luanne Rodgers, Laboratory Scientist, Vitalis Kupara, State Registered Nurse (SRN), Mebbina Muswera, State Registered Nurse and State Registered Midwife (SRN, SRM), Sarah Vundhla, State Registered Nurse and State Registered Midwife (SRN, SRM), Shirley Tshimanga, State Registered Nurse and State Registered Midwife (SRN, SRM).

This study could not have been conducted without the gracious support and collaboration of the staff and patients of the following clinics: Harare, Mbare and Budiriro clinics; Bulawayo, Nkulumane and Khami Road Clinics; Beitbridge, Dulibadzimu Clinic; Gutu, Gutu Rural Hospital.

The Zimbabwe STI Etiology Study was supported by funds from the President’s Emergency Plan for AIDS Relief (PEPFAR) through a cooperative agreement between the U.S. Centers for Disease Control and Prevention and the University of Zimbabwe Department of Community Medicine SEAM Project under the terms of Cooperative Agreement Number IU2GGH000315-01.

Conflict of Interest and Sources of Funding: None declared.

Correspondence: Z. Mike Chirenje, MD, FRCOG, Clinical Trial Unit, University of Zimbabwe College of Health Sciences, 15 Phillips, Belgravia, Harare, Zimbabwe. E-mail: mchirenje@uzchs-ctu.org.

Received for publication September 9, 2017, and accepted November 21, 2017.

Symptomatic vaginal discharge is a common gynecological condition among women of child-bearing age accounting for an estimate of 5 to 10 million clinic visits per year throughout the world.1 Vaginal discharge may be a normal occurrence in pregnancy, menstruation, or use of estrogen containing hormonal contraception.2–4 Abnormal vaginal discharge occurs commonly among women with bacterial vaginosis (BV), candidiasis, trichomoniasis, chlamydia, and gonorrhea.2,5 The presence of abnormal vaginal discharge may also be associated with multiple adverse reproductive tract outcomes that include pelvic inflammatory disease, infertility, ectopic pregnancy, chronic pelvic pain, and a greater than 2- to 3-fold increased risk of human immunodeficiency virus (HIV) acquisition.6–10 In most clinical settings, the management of vaginal discharge is mainly syndrome driven and empirical due to limited point-of-care diagnostic tools to differentiate causative etiologies.4,7,10–12 These empirical regimens commonly cover a spectrum of pathogens that are identifiable causes of sexually transmitted infections (STIs) and reproductive tract infections (RTIs). Reproductive tract infections are typically endogenous infections caused by overgrowth of organisms present in the genital tract.

In 2015, a total of 252,406 adult STI cases were reported syndromically to the Zimbabwe Ministry of Health and Child Care of which 79,371 (31.4%) were women with vaginal discharge (unpublished data). The 2013 Zimbabwe STI treatment guidelines recommend that all women presenting with vaginal discharge should receive a course of oral metronidazole tablets (400 mg or 500 mg orally, 3 times a day for 7 days OR 2 g orally, single dose) as empirical treatment for bacterial vaginosis and trichomoniasis. Additional antifungal treatment using miconazole (200 mg vaginal pessaries once at night for 3 days) or clotrimazole vaginal tablets (100 mg at night for 7 days) is only indicated when clinical manifestations of candidiasis are apparent. Women presenting with mucopurulent vaginal discharge are recommended a regimen that includes kanamycin (2 g intramuscularly, single dose) or ceftriaxone (250 mg intramuscularly, single dose) in addition to doxycycline (100 mg orally, twice a day for 7 days) as empirical treatment for Neisseria gonorrhoeae and Chlamydia trachomatis.13

Syndromic management of symptomatic vaginal discharge is based on clinical signs and symptoms with potential risk of overtreatment and development of antimicrobial resistance in the absence of identified etiological organisms.1,4,7,10 To monitor epidemiological shifts in the etiology of vaginal discharge, it is essential to conduct periodic surveys to provide evidence-based treatment strategies to policy makers.12

The purpose of this study was to establish the etiology of vaginal discharge syndrome among women presenting with symptoms of vaginal discharge in Zimbabwean clinics. The results will be used to assess the continued applicability of existing syndromic treatment guidelines in Zimbabwe.

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MATERIALS AND METHODS

Study Design

This is a substudy of the Zimbabwe STI Etiology study conducted in 2014 to 2015 whose main objective was to establish the etiology of STI syndromes in Zimbabwe, including male urethral discharge syndrome, male and female genital ulcer disease, and vaginal discharge syndrome. Results of the urethral discharge and genital ulcer disease substudies have been published in this journal previously14,15 and a general description of the study’s methodology and study population is available online.16 This article reports on female study participants presenting with vaginal discharge.

The full study protocol including consent forms and questionnaires is available online.17 Six regionally and ethnically diverse STI clinics were selected. Two clinics were selected from Harare (Mbare and Budiriro), 2 from Bulawayo (Nkulumane and Khami Road), 1 from border town Beitbridge (Dulibadzimu), and 1 from a rural community (Gutu Rural Hospital). More details on clinic selection can be found in the study protocol.17

Through convenience sampling, trained nurses recruited 200 consecutively eligible women aged 18 to 53 years presenting with vaginal discharge at the study clinics. Based on statistical considerations as well as limited resources available to the study, we felt that a sample size of 200 would strike an acceptable balance between available study resources and the need for precision.17

Sexually active women presenting with subjective complaints of abnormal vaginal discharge were included in the study. Patients were ineligible if they did not speak Shona, English, or Ndebele (the 3 major languages spoken in Zimbabwe); if they were unable to provide consent; or if they had received treatment for STIs in the previous 4 weeks. Study enrolment was conducted from June 2014 to April 2015.

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Study Procedures

Patients who met the eligibility criteria underwent a consenting process and were asked to sign an informed consent form covering all study procedures. The study nurse then completed a paper copy questionnaire that included demographic information, sexual history, description of symptoms, history of STI/HIV and current use of medications. Each consenting patient underwent genital examination including speculum insertion and received vaginal discharge pH testing by a strip method. Four vaginal swabs were collected, one of which was used to make a smear for Gram stain. A blood sample was taken for HIV testing, among those who consented to this test. All patients were treated for STIs according to the 2013 Zimbabwe STI treatment guidelines.13 Paper data were reviewed and transcribed into a computer based data system on hand held devices. Data were uploaded at least daily from study sites to an online secure central database.

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Laboratory Procedures

All specimens were kept refrigerated (2–8°C) and shipped in a cooler box with cooling packs the same day or overnight to the receiving laboratory at Wilkins Hospital in Harare where the specimens were kept refrigerated until further processing. Vaginal swabs were stored in a freezer at −70°C and batched for shipment. One swab was shipped to the STI reference laboratory at the National Institute for Communicable Diseases in Johannesburg, South Africa to test for N. gonorrhoeae, C. trachomatis, T. vaginalis, and Mycoplasma genitalium using an in-house developed multiplex chain reaction (M-PCR) assay developed at the Centers for Disease Control and Prevention (Atlanta, GA).5 The other two vaginal swabs were shipped to two local laboratories to perform additional testing for N. gonorrhoeae and C. trachomatis by nucleic acid amplification testing (NAAT) using Becton Dickinson ProbeTec (BD Molecular Diagnostics, Franklin Lakes, NJ) at the University of Zimabwe College of Health Science—Clinical Trial Unit Central Laboratory in the Obsterics and Gynaecology department of the University of Zimbabwe and using GeneXpert (Cepheid, Sunnyvale, CA) at the Flowcytometry laboratory in Harare. For the purposes of comparisons with T. vaginalis and M. genitalium in this study, we only included results from the M-PCR. However, concordance between M-PCR with Probetec and GeneXpert (ie, the 3 tests were either all positive or all negative) was greater than 95% for both gonorrhea and chlamydia. A comparative study has been presented elsewhere.18

Human immunodeficiency virus testing was performed at the study laboratory at Wilkins Hospital in Harare using the standard rapid testing algorithm used in Zimbabwe. (HIV testing in Zimbabwe follows a standard algorithm of the following HIV rapid tests: [1] initial test by First Response HIV 1-2.O; Premier Medical Corporation, Daman, India; [2] confirmatory test by Alere Determine HIV 1/2; Alere, Waltham, MS, if the initial test result is positive; and [3] INSTI HIV1/HIV2; Biolytical, Richmond BC, Canada, as a tiebreaker if the initial and confirmatory tests are discrepant.) Syphilis testing using treponemal and nontreponemal assays were also conducted; however, reporting on results fell outside the scope of this article and will be presented elsewhere. Gram staining was also conducted in the laboratory at Wilkins Hospital to diagnose candidiasis and bacterial vaginosis. Candidiasis was defined as the presence of budding yeast or pseudo-hyphae and bacterial vaginosis was diagnosed using the Nugent criteria19; a score of 0 to 3 was considered normal, a score of 4 to 6 was considered intermediate, and a score of 7 to 10 was classified as bacterial vaginosis. Although Gram stain is an insensitive test for gonorrhea, the presence of intracellular Gram-negative diplococci organisms on Gram stain was recorded. All testings were done using standard operating procedures. More detail on laboratory procedures can be found in the online supplement.17

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Data Analysis

Data on participant demographics and sexual health and STI history were analyzed using SAS software (Cary, NC). Tests for statistical significance testing included χ2 and Fisher exact test for categorical variables and Student t test for continuous variables.

Because we assumed the possibility of variations and clustering by clinic, we did not attempt to generalize the relative prevalence of pathogens and conditions associated with vaginal discharge in our study and hence do not present 95% confidence intervals.

For purposes of the analysis in this article, clinics were combined into 3 regions: Harare, Bulawayo, and Beitbridge/Gutu.

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Ethical Considerations

The protocol, consent forms, and questionnaires were reviewed and approved by the University of Zimbabwe Joint Research and Ethics Committee of Parirenyatwa Central Hospital, the Medical Research Council of Zimbabwe and the US Centers for Disease Control and Prevention.

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RESULTS

Demographics and Risk Factors

Selected demographic characteristics and risk factors among women presenting with vaginal discharge are presented in Table 1. Of the 200 enrolled women, 69 (34.5%) were from Harare including 19 pregnant women, 69 (34.5%) were from Bulawayo including 1 pregnant woman, 59 (29.5%) were from Beitbridge and 3 (1.5%) from Gutu. Women recruited were predominantly Shona (63.5%); however, as expected women recruited in Bulawayo were significantly more Ndebele, given that Ndebele is the predominant population in the region. The mean and median ages were 27.6 years and 26 years, respectively. Sixty-two percent of women were married, and only 12.5% were employed. Previous history of STI was reported among 27.9% of the patients. In the preceding 3 months, 7.5% of women reported multiple sex partners, whereas 8.5% reported sex work. Absence of condom use at last sex with main and casual partner was reported by 71.6% and 66.9% of women, respectively. Participants from Beitbridge or Gutu were significantly more likely to report sex work and having more than one partner in the previous 3 months.

TABLE 1

TABLE 1

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Gram Stain Results

Gram stain results were available for 198 women. Nugent scores were as follows: 0 to 3, 75 (37.9%); 4 to 6, 70 (35.3%); and 7 to 10, 53 (26.8%). Forty-one (20.7%) women had Gram stain evidence of yeast infections and 18 (9.1%) women had evidence of gonorrhea. When excluding women with a Gram stain diagnosis of yeast infection or gonorrhea, 49 (24.7%) women were diagnosed with bacterial vaginosis based on a Nugent score of 7 or higher and 90 (45.5%) had a normal Gram stain.

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M-PCR Results

The M-PCR results were available for all 200 women, and pathogens were detected in 90 (45.0%) women, including N. gonorrhoeae (48/200, 24.0%), T. vaginalis (38/200, 19.0%), C. trachomatis (28/200, 14.0%), and M. genitalium (14/200, 7.0%). There were no significant variations in prevalence of STI etiologies across recruitment sites (Table 2). All 18 women with gonorrhea identified on Gram stain tested positive for N. gonorrhoeae by M-PCR.

TABLE 2

TABLE 2

Of the 90 women who had pathogens identified, 62 (68.9%) had a single infection, 18 (20.0%) had a dual infection, and 10 (11.1%) had three infections (Fig. 1). Most coinfections were a combination of gonorrhea with chlamydia and trichomonas (n = 9), gonorrhea and chlamydia (n = 7), and gonorrhea and trichomonas (n = 6). In addition, 24 (49%) of 49 women with bacterial vaginosis but only 10 (24.4%) of 41 women with candidiasis had at least one STI (P < 0.05).

Figure 1

Figure 1

When analyzing the etiology of vaginal discharge in our study by each component of the typical syndromic management for this condition (ie, a combination of kanamycin, doxycycline and metronidazole), the kanamycin component covered 48 (24.0%) women in the sample who had gonorrhea, the doxycycline component covered 60 (30.0%) women who either had gonorrhea or chlamydia, and the metronidazole component covered 77 (38.5) women who either had bacterial vaginosis or trichomoniasis. Combined, the syndromic management regimen covered 115 (57.5%) women in the sample who had gonorrhea, chlamydia, M. genitalium, trichomoniasis, or bacterial vaginosis, whereas 85 (42.5%) women were treated without such diagnosis. The treatment components in the current algorithm13 did not include a macrolide; therefore 14 (7%) of 200 women with Mycoplasma genitalium may have received suboptimal treatment.

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pH

pH assessment was available for 191 (95.5%) of 200 women. The mean pH was 5.2 (median, 5.3; interquartile range, 0.5). Using the median pH value, 82 (42.9%) women had a pH lower than 5.3 and 109 (57.1%) had a pH of 5.3 or higher. Only 8 women in the study had a pH of 4.5 or less. Associations of pH at the 5.3 cutoff with etiologic conditions linked to vaginal discharge is presented in Table 3. Higher pH was statistically significantly associated with a diagnosis of BV and gonococcal infection and marginally associated with chlamydial infection. Lower pH was associated with yeast infection or normal findings. There was no association with trichomonas infection. Performance of the pH test (at the 5.3 cutoff) to predict any condition covered under the syndromic guidelines (ie, BV or infection with N. gonorrhoeae, C. trachomatis, T. vaginalis, or M. genitalium) were as follows: sensitivity, 73 (67.6%) of 108; specificity, 47 (56.6%) of 83; predictive value of a high PH, 73 (67.0%)of 109; predictive value of low pH, 47 (57.3%) of 82.

TABLE 3

TABLE 3

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HIV Infection

Of the 158 women who consented to HIV testing, 64 (40.5%) were HIV infected. The burden of STIs was higher among women with HIV infection compared to HIV uninfected women (54.7% vs 37.2%, P < 0.05). Table 4 shows association of HIV with STIs and RTIs. Women with T. vaginalis infection were more likely to be HIV infected than women without T. vaginalis infection (59.4% versus 35.7%, P < 0.05). Women with gonorrhea were also more likely to be HIV infected compared to women without gonorrhea (54.1% versus 36.4%, P = 0.05). Although not significantly different, women with chlamydia infection were less likely to be HIV infected compared with women without chlamydia infection (22.7% vs 43.4%, P = 0.06); however, this association disappeared when controlling for age (data not shown).

TABLE 4

TABLE 4

There was no association between HIV infection and diagnosis of bacterial vaginosis or yeast infection.

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DISCUSSION

The purpose of this substudy was to establish the etiology of vaginal discharge syndrome among women presenting to selected Zimbabwe STI clinics to assess the adequacy of current treatment guidelines against the prevalent causes of vaginal discharge.

Bacterial vaginosis (24.7%) was the most prevalent cause identified among women presenting with vaginal discharge syndrome. The predominance of bacterial vaginosis as a common cause of vaginal discharge syndrome is an observation that has been reported in several other studies among women with RTIs.4–7,10 Detection of STIs by M-PCR was most common for N. gonorrhoeae (24.0%), T. vaginalis (19.0%), and C. trachomatis (14.0%). The observed higher burden of N. gonorrhoeae compared with C. trachomatis in our study is in contrast to previous studies among asymptomatic women conducted in Zimbabwe20,21 and in sub-Saharan Africa which revealed a higher burden of C. trachomatis than N. gonorrhoeae.22 This could be a manifestation resulting from the more symptomatic nature of gonorrhea than chlamydia, such that women infected with N. gonorrhoeae are more likely to present to a health care facility.23

The presence of multiple concurrent infections was most commonly identified among women infected with N. gonorrhoeae, C. trachomatis and T. vaginalis, a manifestation that typically presents with mucopurulent vaginal discharge. This study also reveals presence of M. genitalium (7.0%). This organism has been reported as a cause of STI in several countries, and an association with HIV has been reported.24–26 Replacing doxycycline with azithromycin in the syndromic treatment algorithm may increase coverage for M. genitalium while still providing adequate coverage for chlamydia (azithromycin is currently listed as an alternative regimen in the Zimbabwe STI treatment guidelines13). However, a 23S rRNA mutation in the M. genitalium ribosome has resulted in rapidly increasing macrolide resistance worldwide, especially in countries that have replaced doxycycline with azithromycin in their STI treatment guidelines, leaving a conundrum for appropriate syndromic management and providing another rationale for etiologic testing.27

A significant number of women presenting with vaginal discharge syndrome were HIV infected (40.5%) compared to HIV prevalence of 15.6% in the general population.28 The burden of STIs was higher among HIV-infected women (54.7% vs 37.2%, P < 0.05). Trichomoniasis was significantly associated with HIV infection, an observation that has been reported in previous studies.29 These data support a recommendation that the Zimbabwe Ministry of Health and Child Care should consider offering HIV testing and linkage to care for all women presenting with symptomatic vaginal discharge. In addition to prompt treatment of STI, partner management for women diagnosed with STI is also crucial to prevent reinfection and ongoing transmission.12,30,31

With regard to the adequacy of syndromic management for vaginal discharge syndrome, our study found that 57.5% of women had a pathogen covered by a combination treatment of kanamycin, doxycycline, and metronidazole. However, the kanamycin component overtreated 76% of women who did not have gonorrhea; the doxycycline component overtreated 70% of women who did not have gonorrhea or chlamydia and the metronidazole component overtreated 61.5% of women who did not have bacterial vaginosis or trichomoniasis. Moreover, treatment for M. genitalium may have been suboptimal. Our results are in agreement with several studies that have observed a relatively low positive predictive value for the detection of STI when using the vaginal discharge syndrome algorithm5,10,11,30 with potential harm for over treatment, antimicrobial resistance, and stigmatization. To avoid the burden of over treatment, there is need for diagnostic capacity at the clinics. Thus affordable point of care testing32 should be considered for STI testing among women presenting with symptomatic vaginal discharge, including basic microscopy to differentiate BV, trichomoniasis, and yeast infections. Also, point of care tests that use nucleic acid amplification technology are increasingly becoming available for tuberculosis/rifampin resistance testing in developing countries and can easily be used for chlamydia and gonorrhea testing on the same platform.

Vaginal pH testing is a simple and cheap test that could be used in the diagnostic differentiation of the vaginal discharge syndrome at the point of care. Indeed, we found statistically significant associations between a pH of 5.3 or greater and BV or infection with N. gonorrhoeae. However, for practical purposes, the diagnostic differentiation proved to be inadequate. Whereas the positive predictive value was quite high (67%) for women with a high pH who had BV or STI, the negative predictive value was low (ie, 42.7% of women with a low pH still had BV or STI).

Given the high burden of STIs among HIV-infected women and the high level of concurrent STIs, there is a high risk of ongoing HIV transmission in this community. In addition to capacitating clinics with STI point-of-care testing, the Zimbabwe Ministry of Health and Child Care should consider expanding programs that promote HIV risk reduction. These include condom use promotion and antiretroviral treatment (ART) counseling. Adherence to ART reduces viral load thereby reducing the chances of HIV transmission to sexual partners.33 In addition, partners should be offered HIV testing; HIV-negative partners should be encouraged to take up preexposure prophylaxis to reduce HIV infection34 and HIV-positive partners should be encouraged to initiate ART as per World Health Organization guidelines.35

Our study should be understood in light of some limitations. First, for the diagnosis of yeast infection and bacterial vaginosis, the study relied on Gram stain testing; on-site wet mount testing was not conducted. This could have resulted in an under-estimation of both conditions. Moreover, 35% of women had intermediate (4–6) Nugent scores, and in one study, 32% of women with intermediate scores progressed to bacterial vaginosis, whereas 30% reverted to normal flora.36 Second, this study did not determine antibiotic susceptibility to confirm the effectiveness of the empirical regimens as recommended by the current local guidelines particularly for N. gonorrhoeae.13 However, a recent study conducted in Zimbabwe showed high levels of N. gonorrhoeae susceptibility to kanamycin and ceftriaxone20 and additional studies are ongoing. Third, this study only enrolled symptomatic women that could have resulted in a bias toward a high prevalence of STIs, and these results can therefore not be generalized to asymptomatic women; thus requiring additional studies among the latter population.

In view of the 42.5% of women with symptomatic vaginal discharge who were overtreated using the current algorithm, we recommend a review of the Zimbabwean STI treatment algorithm with a focus on point-of-care testing. Point-of-care testing, especially the use of nucleic acid amplification testing devices that are becoming increasingly available, would also allow for testing of asymptomatic women. Because STIs among women, including gonorrhea and chlamydia infections are often asymptomatic, such an approach would have a greater impact on STI control than the current approach, depending on syndromic management.

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