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A Novel Method to Increase the Viability of Trichomonas vaginalis in Urine

Shafir, Shira C. PhD*; Sorvillo, Frank J. PhD*†; Upcroft, Jacqui A. PhD; Upcroft, Peter PhD

Sexually Transmitted Diseases: July 2007 - Volume 34 - Issue 7 - p 485-487
doi: 10.1097/01.olq.0000249724.72816.a0
Article

Objectives: Two of the major diagnostic methods for Trichomonas vaginalis, wet mount and culture, rely on the continued viability of the organism. Methods to increase the viability of T. vaginalis in urine are needed.

Goal: The goal of this study was to develop a method that increases the time of viability of T. vaginalis in urine.

Study Design: Urine samples were inoculated with trichomonads, held at either room temperature or 37 °C, and processed through a column and frit, which was then placed in either a tube of culture medium containing antibiotics or a TV InPouch.

Results: The column and polyethylene frit system was found to increase the duration of viability for T. vaginalis from urine specimens at least 6-fold.

Conclusion: This novel method, which uses a column and frit system to increase the duration of viability of the organism, has the potential to increase the sensitivity of diagnostic tests.

Use of a column and polyethylene frit can increase the time of viability of Trichomonas vaginalis in urine. Continued viability of the organism is essential for most diagnostic tests.

From the *Department of Epidemiology, UCLA School of Public Health, Center for Health Sciences, Los Angeles, California; the †Data Collection and Analysis Unit, Los Angeles County Department of Health Sciences, Los Angeles, California; and the ‡Queensland Institute of Medical Research, The University of Queensland, Brisbane, Herston, Queensland, Australia

The authors gratefully acknowledge Dr. Lawrence Ash for assistance in experimental design and Dr. Patricia Johnson for supplying the T1 strain. The authors also acknowledge Dr. Roger Detels and Dr. Barbara Visscher for assistance in manuscript review.

Correspondence: Shira C. Shafir, PhD, UCLA School of Public Health, Department of Epidemiology, Center for Health Sciences Room 41-275, 650 Charles E. Young Drive, Los Angeles, CA 90024. E-mail: sshafir@ucla.edu.

Received for publication June 24, 2006, and accepted September 7, 2006.

© Copyright 2007 American Sexually Transmitted Diseases Association