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00152232-201500001-00028ArticleSpine: Affiliated Society Meeting AbstractsSpine: Affiliated Society Meeting Abstracts© 2015 Lippincott Williams & Wilkins, Inc.2015 p 28THE EFFECT OF VANCOMYCIN POWDER ON BONE HEALING IN A RAT SPINAL ARTHRODESIS MODELO38ArticleMendoza, Marco C. MD; Sonn, Kevin MD; Kannan, Abhishek S. BS; Bellary, Sharath MD; Mitchell, Sean M. BS; Singh, Gurmit BS; Park, Christian BS; Yun, Chawon PhD; Yun, Jonghwa; Ghosh, Anjan; Stock, Stuart R. PhD; Hsu, Erin L. PhD; Hsu, Wellington K. MDNorthwestern University Department of Orthopaedic SurgeryINTRODUCTION: Surgical site infections (SSIs) after spinal surgery occur in 1-10% of patients. Such complications are devastating to patients and the healthcare system. Staphylococcus aureus is the most common organism responsible for SSIs, and vancomycin powder has the potential to serve as a simple, cost-effective solution to the problem. Although in vitro studies suggest that vancomycin is cytotoxic to differentiating osteoblasts, the effect of vancomycin powder application on the rates of spinal arthrodesis has not been properly evaluated. METHODS: Thirty-six female rats underwent a posterolateral lumbar spinal fusion at the L4 and L5 vertebrae. Fusion was elicited via implantation of an absorbable collagen sponge containing 3 μg rhBMP-2. Rats were divided into three groups: no vancomycin (control), standard dose vancomycin, and high dose vancomycin, which was applied to the fusion bed. Spines were harvested and evaluated at 8 weeks post-operative using radiographs, fusion scoring, microCT, and histologic analysis. RESULTS: Qualitative radiographs demonstrated equivalent bridging bone formation in all groups. No significant differences in fusion scores were seen in the standard-dose (2.25) or high-dose (2.13) treatment groups relative to untreated control animals (1.78) (Figure 1-A). Similarly, fusion rates were not significantly different between vancomycin-treated animals (100% for both groups) and control animals (92%) (Figure 1-B). Quantification of new bone formation via microCT imaging revealed no significant differences in the volume of newly regenerated bone among groups (Figure 1-C). DISCUSSION: This study demonstrates that vancomycin powder does not inhibit fusion rates at an equivalent wt% dose to what is routinely used by surgeons. Moreover, bone formation and fusion rates were not reduced even after administration of a vancomycin dose that is ten-fold higher than which is typically administered clinically.JOURNAL/spinea/04.03/00152232-201500001-00028/figure1-28/v/2021-02-17T195540Z/r/image-jpeg Fusion scores from blind manual palpation analysis at 8 weeks post-operation. (B) Fusion rates of each treatment-based manual palpation scores, where an average score greater than or equal to 1.0 was considered solidly fused. No significant difference was found in fusion scores among the standard dose, high dose vancomycin and control groups. (C) The successfully fused specimens were analyzed by microcomputed tomography (μCT) to compare fusion mass volume (mm3). There was statistically no significant difference between the vancomycin-treated groups relative to no-vancomycin controls (control vs. standard dose vancomycin: p = 0.57; control vs. high dose vancomycin: p = 0.53) Fusion scores from blind manual palpation analysis at 8 weeks post-operation. (B) Fusion rates of each treatment-based manual palpation scores, where an average score greater than or equal to 1.0 was considered solidly fused. No significant difference was found in fusion scores among the standard dose, high dose vancomycin and control groups. (C) The successfully fused specimens were analyzed by microcomputed tomography (μCT) to compare fusion mass volume (mm3). There was statistically no significant difference between the vancomycin-treated groups relative to no-vancomycin controls (control vs. standard dose vancomycin: p = 0.57; control vs. high dose vancomycin: p = 0.53)<strong xmlns:mrws="http://webservices.ovid.com/mrws/1.0">THE EFFECT OF VANCOMYCIN POWDER ON BONE HEALING IN A RAT SPINAL ARTHRODESIS MODEL</strong>: <strong xmlns:mrws="http://webservices.ovid.com/mrws/1.0">O38</strong>Mendoza Marco C. MD; Sonn, Kevin MD; Kannan, Abhishek S. BS; Bellary, Sharath MD; Mitchell, Sean M. BS; Singh, Gurmit BS; Park, Christian BS; Yun, Chawon PhD; Yun, Jonghwa; Ghosh, Anjan; Stock, Stuart R. PhD; Hsu, Erin L. PhD; Hsu, Wellington K. MDArticleArticle2015p 28