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00152232-201500001-00030ArticleSpine: Affiliated Society Meeting AbstractsSpine: Affiliated Society Meeting Abstracts© 2015 Lippincott Williams & Wilkins, Inc.2015 p 30Suppressive effect of anti-interleukin-6 receptor antibody on neuropathic pain-related factor in sciatic nerve-injured mice.O11ArticleNakayama, S.; Sainoh, T.; Yamauchi, K.; Orita, S.; Kubota, G.; Inage, K.; Sato, J.; Fujimoto, K.; Shiga, Y.; Takahashi, K.; Ohtori, S.Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba UniversityINTRODUCTION: The pathomechanism of neuropathic pain is associated with inflammatory cytokines such as interleukin-6 (IL-6). The aim of the present study was to evaluate the efficacy of local administration of the anti-IL-6-receptor antibody MR16-1 in a sciatic nerve-injured mouse model by behavior and immunohistology. METHODS: Right sciatic nerve pinch was performed in C57BL/6 mice and 3 groups were created: a sham (non-pinch) group, given 10-μL saline locally; a pinch group, given 10-μL saline after nerve pinch; and the MR16-1 group, given 10-μL MR16-1 after nerve pinch. Pain behavior was assessed by gait analysis 1 week after operation. Injured sciatic nerves were then extracted and nerve degeneration and demyelination were evaluated by histopathology. The L4-6 dorsal root ganglia (DRG) were harvested and immunostained for calcitonin gene-related peptide (CGRP) and activating transcription factor 3 (ATF3). Rates of CGRP- and ATF3-immunoreactivity (ir) in DRG neurons from each group were statistically analyzed. RESULTS: On gait analysis, contact time and pressure of ipsilateral limbs was decreased in pinch mice and improved in MR16-1 mice (p<0.05). Inflammatory cell infiltration, neural cell size change, and denatured demyelination at the distal insult were observed in injured nerves; the insult was mitigated in the MR16-1 group. Pinch group neurons exhibited a significantly higher rate of CGRP-ir vs. the sham group (p<0.01), but there was no difference between sham and MR16-1 mice. Similarly, ATF3-ir was more common in pinch mice than in sham mice (p<0.05), and was suppressed in MR16-1 mice. DISCUSSION: IL-6 antagonism promoted pain relief and repair of damaged tissue during acute-phase nerve injury. We need to explore more pathomechanism of IL-6 antagonism in the future study, which leads to possible novel treatment for neuropathic pain.<strong xmlns:mrws="http://webservices.ovid.com/mrws/1.0">Suppressive effect of anti-interleukin-6 receptor antibody on neuropathic pain-related factor in sciatic nerve-injured mice.</strong>: <strong xmlns:mrws="http://webservices.ovid.com/mrws/1.0">O11</strong>Nakayama S.; Sainoh, T.; Yamauchi, K.; Orita, S.; Kubota, G.; Inage, K.; Sato, J.; Fujimoto, K.; Shiga, Y.; Takahashi, K.; Ohtori, S.ArticleArticle2015p 30