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00152232-201000002-00025AbstractSpine: Affiliated Society Meeting AbstractsSpine: Affiliated Society Meeting Abstracts© 2010 Lippincott Williams & Wilkins, Inc.
p 65Mechanism of Osteoporosis in Adolescent Idiopathic Scoliosis: Experimental Scoliosis in Pinealectomized Chickens†Paper #25Podium Presentation AbstractsMachida, Masafumi MD; Katon, Hiroyuki; Kono, Hitoshi; Kaneko, Shinjiro; Fukuda, Kentaro; Saito, Masashi; Takemitsu, MasakazuJapanSummary: The radiological and histological changes in the cervical spine were evaluated in experimental scoliosis in chickens. Melatonin deficiency plays a crucial role in the development of scoliosis and osteoporosis.Introduction: The radiological and histological changes in the cervical spine were evaluated in experimental scoliosis in chickens. Melatonin deficiency plays a crucial role in the development of scoliosis and osteoporosis.Methods: Forty new-hatched broiler chickens were divided into equal four groups: sham operated chickens serving as control (CNT); pinealectomized chickens (PNX); sham operated (CNT+MLT) and pinealectomized chickens (PNX+MLT) that received intraperitoneal administration of melatonin (MLT) (8 mg/kg BW) at 22:00 hr daily. Surgeries were performed at the age of 3 days. Before killing the chickens at 2 months of age, blood samples were collected in the middle of the light-dark cycle and melatonin levels were measured by radioimmunoassay. Postmortem X-rays were examined for the presence of a scoliosis, and microCTs were taken to evaluate the microstructure of the cervical vertebrae. A midsagittal section of the scanned cervical vertebrae was stained with HE and TRAP to evaluate osteoblasts and osteoclasts respectively.Results: Scoliosis developed at the thoracic spine in all PNX and in 2 of the PNX+MLT groups. MLT levels in the PNX group were reduced remarkably, while normal levels were restored in the PNX+MLT group and were normal in the CNT and CNT+MLT groups. The data from microCT wad evaluated for several architectural parameters such as bone volume fraction (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), star volume of marrow space (V*m.space) and of the trabeculae (V*tr), and revealed that pinealectomized chickens had generalized osteoporosis. The number of osteoblasts was significantly decreased in the PNX group, while no significant difference observed between the CNT and PNX+MLT groups. The number of osteoclasts were similar in all groups.Conclusion: Our results suggest that MLT stimulates osteoblast proliferation and that MLT deficiency plays a crucial role in the development of scoliosis and osteoporosis. The restoration of MLT levels prevented the development of scoliosis and osteoporosis, indicating that the levels of MLT might be the key to scoliotic deformity and osteoporosis in AIS.Significance: This is the first report indicating that the levels of MLT might be the key to scoliotic deformity and osteoporosis in AIS.<strong xmlns:mrws="http://webservices.ovid.com/mrws/1.0">Mechanism of Osteoporosis in Adolescent Idiopathic Scoliosis: Experimental Scoliosis in Pinealectomized Chickens</strong>: <strong xmlns:mrws="http://webservices.ovid.com/mrws/1.0">†Paper #25</strong>Machida Masafumi MD; Katon, Hiroyuki; Kono, Hitoshi; Kaneko, Shinjiro; Fukuda, Kentaro; Saito, Masashi; Takemitsu, MasakazuPodium Presentation AbstractsPodium Presentation Abstractsp 65
