ISSLS Prize Winner

Adaptations to the Multifidus Muscle in Response to Experimentally Induced Intervertebral Disc Degeneration

Abstract

Study Design. 

Basic science study of the rabbit multifidus muscle response to intervertebral disc degeneration.

Objective. 

To assess changes in passive mechanical properties, associated protein structure, and histology of multifidus in response to disc degeneration produced by experimental needle puncture.

Summary of Background Data. 

Relationships have been reported between muscle dysfunction and low back injury; however, little is known about the cause and effect of such relationships.

Methods. 

Twelve rabbits were studied; 4 in each of 3 groups: control, 4-weeks postintervertebral disc injury (4-week disc degeneration), and 12-weeks postintervertebral disc injury (12-week disc degeneration). Single multifidus fibers and bundles of fibers were isolated and tested for slack sarcomere length and elastic modulus. Titin isoform mass, myosin heavy chain distribution, and muscle histology were also examined.

Results. 

Compared to control, individual muscle fibers were 34% stiffer and fiber bundles 107% stiffer in the 12-week disc degeneration group. No changes were detected at 4-week disc degeneration. No statistically significant change was found for MHC distribution in the 12-week disc degeneration group when compared to control, whereas titin isoforms were larger (P < 0.05) in the 12-week disc degeneration group. Histology revealed select regions of multifidus, at 12-week disc degeneration, with increased space between bundles of fibers, which in some instances was partly occupied by adipose tissue.

Conclusion. 

Multifidus becomes stiffer, both in individual fibers and fiber bundles, in response to experimentally induced intervertebral disc degeneration. This cannot be explained by change in fiber-type due to reduced muscle use, nor by the increased size of the protein titin (which would reduce stiffness). We hypothesize that fiber bundles become stiffer by proliferation and/or reorganization of collagen content within the muscle but the basis for fiber stiffening is not known.

In Brief

Multifidus muscle single fibers and fiber bundles were stiffer 12 weeks after intervertebral disc degeneration was induced in rabbits. Histology indicated that extracellular matrix proliferation/reorganization was likely the primary cause of fiber bundle stiffening. This provides direct evidence for changes in mechanical properties of back muscles in response to disc degeneration.