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Glycosaminoglycan Chemical Exchange Saturation Transfer of Lumbar Intervertebral Discs in Healthy Volunteers

Schleich, Christoph, MD*; Müller-Lutz, Anja, PhD*; Eichner, Markus, BSc*; Schmitt, Benjamin, PhD; Matuschke, Felix, BSc*; Bittersohl, Bernd, MD; Zilkens, Christoph, MD; Wittsack, Hans-Jörg, Prof*; Antoch, Gerald, Prof*; Miese, Falk, MD*

doi: 10.1097/BRS.0000000000001144
DIAGNOSTICS

Study Design. Evaluation of a new quantitative imaging technique in a prospective study design.

Objective. To assess glycosaminoglycan (GAG) content of lumbar intervertebral discs (IVDs) in healthy volunteers with chemical exchange saturation transfer (CEST).

Summary of Background Data. Biochemical alterations of lumbar discs are present before the appearance of morphological changes. GAG loss plays a central role in these degenerative processes.

Methods. Lumbar intervertebral discs of healthy controls (26 women, 22 men; mean age 31 ± 8 years; range: 21–49 years) without lumbar back pain were examined at a 3 Tesla magnetic resonance imaging (MRI) scanner in this prospective study. None of the participants were overweight or had previous surgery of the lumbar spine. The MRI protocol included standard morphological, sagittal and transversal T2-weighted (T2w) images to assess Pfirrmann score and to detect disc disorders according to the Combined Task Force classification of five lumbar IVDs (L1 to S1). A prototype glycosaminoglycan chemical exchange saturation transfer (gagCEST) sequence was applied to measure GAG content of the nucleus pulposus (NP) and annulus fibrosus (AF) by identifying the magnetization transfer asymmetry ratio (MTRasym) in a region-of-interest analysis. Morphological and biochemical imaging analysis were statistically tested for quantitative differences between different grades of IVD degeneration and disc disorders.

Results. gagCEST values of NP demonstrated a significant negative correlation with morphological Pfirrmann score (r = −0.562; P < 0.0001). The MTRasym values were higher in non-degenerative lumbar IVDs (Pfirrmann 1–2) compared with degenerative lumbar discs (Pfirrmann 3–5; 2.92% ± 1.42% vs. 0.78% ± 1.38%; P < 0.0001). The MTRasym values of NP were significantly higher in normal appearing discs compared with herniated IVDs (2.83% ± 1.52% vs. 1.55% ± 1.61%; P < 0.0001). We found a significant negative correlation between gagCEST values and the graduation of disc herniation (r = −0.372; P < 0.0001).

Conclusion. Biochemical imaging with gagCEST distinguished morphologically degenerative from non-degenerative lumbar IVDs (in NP and AF) of healthy volunteers at a clinical 3T-MRI system. The depletion of GAG content in degenerative lumbar discs correlated significantly with the morphological disc classification. We could demonstrate that disc disorders, such as protrusion and extrusion, were accompanied by lower GAG content.

Level of Evidence: 2

*Medical Faculty, Department of Diagnostic and Interventional Radiology, University Dusseldorf, Dusseldorf, Germany

Siemens Ltd, Australia

Medical Faculty, Department of Orthopedics, University Dusseldorf, Düsseldorf, Germany.

Address correspondence and reprint requests to Anja Müller-Lutz, PhD, University Dusseldorf, Medical Faculty, Department of Diagnostic and Interventional Radiology, Moorenstr. 5, 40225 Dusseldorf, Germany; E-mail: anja.lutz@med.uni-duesseldorf.de

Received 20 May, 2015

Revised 9 July, 2015

Accepted 3 August, 2015

The manuscript submitted does not contain information about medical device(s)/drug(s).

No funds were received in support of this work.

Relevant financial activities outside the submitted work: employment, payment for lectures.

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