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The Contribution of the Omentum to the Outcome From Sepsis

An Experimental Animal Study

Wang, Andrew W.*; Cauvi, David M.; Hawisher, Dennis; Reyes, Tony; Coimbra, Raul†,§; Bickler, Stephen||; De Maio, Antonio†,¶

doi: 10.1097/SHK.0000000000001311
Basic Science Aspects
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ABSTRACT The omentum is a large mesenchymal fibro-fatty tissue with remarkable healing capability. It is also rich in immune cells, including macrophages and lymphocytes, within particular structures named milky spots. Clinical observations indicate a high incidence of peritonitis after the removal of the omentum suggesting that it may play a role in sepsis. To test this possibility, male CD-1 mice underwent simultaneous omentectomy and cecal ligation and puncture (CLP), omentectomy-sham operation and CLP alone, and mortality was documented within 72 h post the insults. A significant increase in mortality was observed in mice subjected to omentectomy and CLP in comparison with CLP alone. Mortality was correlated with an increase in cytokine gene expression within the lung after omentectomy and CLP as opposed to CLP alone. However, no differences in bacterial load were observed within the peritoneum or blood between groups. To test the long-term effect of omentectomy, mice were subjected to omentum removal or sham operation, allowed to recover from surgery for 14 or 28 days, and then both were subjected to CLP. In these cases, no differences in mortality were observed between the groups suggesting that the lack of omentum triggers a compensatory mechanism. Finally, omentectomy and sham operation altered the composition of peritoneal immune cells with the disappearance of F4/80high macrophages and the appearance of a new population of F4/80low macrophages within 1 or 14 days post-surgery. The F4/80high positive cells reappeared after 28 days following the procedures. All of these observations suggest that the omentum plays an early role in the outcome from sepsis.

*Department of Surgery, Naval Medical Center San Diego, San Diego, California

Division of Trauma, Critical Care, Burns and Acute Injury, Department of Surgery, School of Medicine, University of California, La Jolla, California

IMSD Program, University of California San Diego, La Jolla, California

§Riverside University Health System Medical Center and Department of Surgery, Loma Linda University School of Medicine, Loma Linda, California

||Division of Pediatric Surgery, Rady Children's Hospital, San Diego, California

Department of Neurosciences, School of Medicine, University of California, La Jolla, California

Address reprint requests to Antonio De Maio, PhD, School of Medicine, University of California San Diego, 9500 Gilman Drive, #0739, La Jolla, CA 92093-0739. E-mail: ademaio@ucsd.edu

Received 30 August, 2018

Revised 14 September, 2018

Accepted 18 December, 2018

This work was supported by NIH grant R01 GM114473-01.

The authors report no conflicts of interest.

© 2019 by the Shock Society