Detection of some virulence genes among clinical Pseudomonas aeruginosa isolates in Baghdad- Iraq

Abstract

Purpose: 

The objectives of the current study are to detect some important virulence genes in Pseudomonas aeruginosa, which isolated from clinical samples in Baghdad's different hospitals.

Methods: 

A total of 40 clinical P. aeruginosa isolates were collected from different hospitals in Baghdad city. The isolates were identified using morphological tests and confirmed by conventional PCR by using specific primer for 16S rDNA gene. To extract DNA from the isolates, a genomic DNA kit was used molecular detection of two virulence genes: ompA and papC, which were amplified by PCR.

Results: 

All 40 isolates were identified morphological and genetic tests. Moreover, the prevalence of virulence genes by PCR demonstrated that that (27.5%) and (17.5%) P. aeruginosa isolates carried the ompA and papC genes, respectively. These genes are associated to biofilm formation. The ompA was the most predominant gene.

Conclusion: 

The current data support the occurrence of P. aeruginosa exhibiting important virulence genes, which indeed make these bacteria provide such a significant threat to public health.