BACTERIOLOGYProduction of blaoxa-23 type genes carrying by Acinetobacter baumannii isolated from hospitalized patients in Tehran, IranRezaei, Maryama; vand Yousefi, Jalila; Harzandi, Naserb; Sharifizadeh, Monirehb; Zahedi bialvaei, Abedc; Rahbar, MohammaddAuthor Information aDepartment of Microbiology. Islamic Azad University, Tehran bDepartment of Microbiology, Karaj Branch, Islamic Azad University, Karaj cStudent Research Committee, Iran University of Medical Sciences dDepartment of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran. Correspondence to Mohammad Rahbar, Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran. Tel: +98 2166728112 13; fax: +98 21 66728113; e-mail: [email protected] Received 23 February, 2020 Revised 13 March, 2020 Accepted 1 April, 2020 Reviews in Medical Microbiology: April 2021 - Volume 32 - Issue 2 - p 102-105 doi: 10.1097/MRM.0000000000000224 Buy Metrics Abstract Carbapenems are potent β-lactam antibiotics, including imipenem and meropenem that is used to treat serious infections caused by Acinetobacter baumannii. The aim of this study was to determine the resistance rate of A. baumannii to imipenem and meropenem by producing OXA-23 gene. Sixty A. baumannii isolates were collected from four hospitals. Susceptibility testing of all A. baumannii isolates was determined by disk diffusion method. After bacterial DNA extraction, PCR was used for detection of blaoxa-51 and blaoxa-23 beta-lactamase genes. According to the result, 98% of A. baumannii isolates were resistant to amikacin, ceftazidime and ceftriaxone. Resistance rates to imipenem and meropenem was 72%. All isolates were resistant to cefixime; however, were susceptible to colistin. PCR method determined the presence of resistance-encoding class D carbapenemase including blaoxa-51 like and blaoxa-23 like in 60 (100%) and 51 (85%) isolates, respectively, which was in agreement with disk diffusion method. Our study revealed a high rate of drug resistance among A. baumannii isolates with presence of blaoxa-51 and blaoxa-23 β-lactamase gens. However, colistin were the effective antimicrobial agents, in vitro. Therefore, the rate of carbapenem-resistant A. baumannii susceptibility profiling highlights the need for a comprehensive Iranian national antimicrobial drug resistance survey to monitor A. baumannii isolates from all parts of the country. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.