Bacterial vaginosis is characterized by a disequilibrium in vaginal microflora with the distinguishing feature of decrease in lactobacilli. The current study aimed to study lactobacilli and bifidobacteria in the vaginal tract of individual with bacterial vaginosis.
For this purpose, vaginal swabs of 108 women, including 92 patients with clinical symptoms of bacterial vaginosis and 16 healthy cases were collected. After extraction of DNA from vaginal samples, quantitative PCR was performed for detect the bacterial load and 16SrRNA gene. PCR was performed for the detection of microorganisms related to sexually transmitted infections, which all samples harboring any of such microorganisms was excluded from study.
There was a positive link between the quantitation of Lactobacillus spp. and Bifidobacterium spp. among patients with bacterial vaginosis and healthy group. Quantitative PCR analysis illustrates that the frequency of Lactobacillus spp. and Bifidobacterium spp. are significantly lower in patients with bacterial vaginosis compared with healthy individuals.
The current study promotes our understanding about the vaginal Lactobacillus spp. and Bifidobacterium spp. among patients and healthy individuals in Iran. Confirming the link between the frequency of lactobacilli and bacterial vaginosis could help us develop new prebiotics and probiotics for this disorder.
aDepartment of Molecularbiology, Cancer Biomedical Center (CBC), Tehran
bShahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan
cMicrobiology Department, Basic Sciences Faculty, Qom Branch, Islamic Azad University, Qom
dDepartment of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz
eDepartment of Microbiology, School of Medicine, Iran University of Medical Sciences
fDepartment of Microbiology, Shahid Beheshti University of Medical Science, Tehran, Iran.
Correspondence to Hossein Dabiri, Department of Microbiology, Shahid Beheshti University of Medical Science, Tehran, Iran. Tel: +98 2166728112x13; fax: +98 21 66728113; e-mail: firstname.lastname@example.org
Received 12 October, 2018
Revised 26 March, 2019
Accepted 10 April, 2019