Cell culture methods are utilized ubiquitously in science and pharmaceutical and biotech industries. Sterility is one crucial factor for maintenance of cells and creation of valid data from experiments. As cells are usually cultured in CO2 incubators, those are one bottleneck in terms of sterility in a cell culture lab. This review gives an overview on the different sterilization procedures for CO2 incubators on the market with emphasize to considerations from a practical point of view. It compares sterilization by dry heat, steam, gas and ultraviolet radiation in terms of validity and practicability in accordance with international standards and regulations and transfers literature consensus about these methods to CO2 incubators. As conclusion, the authors give recommendations for a sterile working environment in line with good cell culture practice.
aInstitute for Experimental Virology, Twincore, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover
bDr Brill + Partner GmbH, Institute for Hygiene and Microbiology, Bremen
cInstitute of Clinical Hygiene, Medical Microbiology and Infectiology, Paracelsus Medical University, Klinikum Nürnberg, Nuremberg
dDepartment for Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany.
Correspondence to Dr Florian H.H. Brill, Dr Brill + Partner GmbH, Institute for Hygiene and Microbiology, Stiegstück 34, DE-22339 Hamburg, Germany. E-mail: Florian.firstname.lastname@example.org
Received 12 July, 2018
Accepted 27 July, 2018