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Detection of prevalent mechanism of extended spectrum β-lactamases, metallo β-lactamases, and AmpC β lactamases-producing Klebsiella pneumoniae in the tertiary care hospital

Alyousef, Abdullah A.a; Khazaal, Saba Saadoonb; Ali, Alaa N. Mohammedb; Hussein, Nadheema Hammoodb; Hussein, Sawsan Mustafa Aloahdc

Reviews in Medical Microbiology: October 2017 - Volume 28 - Issue 4 - p 133–139
doi: 10.1097/MRM.0000000000000117

Increasing trends of resistance to antibiotics because of production of β-lactamases, such as carbapenamase-mediated resistance and extended spectrum β-lactamases (ESBL), are the most widespread mechanisms documented in Klebsiella pneumoniae. The objective of this study was to understand the prevalent mechanisms of resistance among the clinical isolates of multidrug-resistant (MDR) K. pneumonia from inpatients of a tertiary care hospital at Baghdad, Iraq. Among the 97 MDR K. pneumoniae isolates, 47.4% (n = 46) isolates showed ESBL production, 38.7% (n = 38) showed metallo β-lactamase (MBL) production, and 22.7% (n = 22) showed both ESBL and MBLs production. Out of 97 MDR K. pneumoniae, 94.3% were found to be biofilm producers. ESBL production was the predominant mechanism of resistance followed by MBL production. Since very limited therapeutic options are available, increasing resistance mechanisms among these organisms is an ‘alarming sign’.

aDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia

bDepartment of Biology, Branch of Biotechnology, College of Science, AL-Mustansiryia, University, Baghdad, Iraq

cCollege of Life Science, Maulana Azad College of Arts and Science, Aurangabad, Maharashtra, India.

Correspondence to Sawsan Mustafa Aloahd Hussein, PhD, College of Life Science, Maulana Azad College of Arts and Science, Aurangabad, India. Tel: +976 5412548562; e-mail:

Received 18 May, 2017

Revised 27 June, 2017

Accepted 7 August, 2017

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