DIAGNOSTIC MICROBIOLOGYRecent advances in the rapid diagnosis of various infectious diseases by PNA FISHWang, PeiAuthor Information Department of Laboratory Medicine, The First People's Hospital of Jingmen, Hubei, China. Received 23 May, 2009 Accepted 8 August, 2009 Correspondence to Dr Pei Wang, Division of Clinical Microbiology, Department of Laboratory Medicine, The First People's Hospital of Jingmen, Hubei 448000, China. Fax: +86 724 2305687; e-mail: [email protected] Reviews in Medical Microbiology: July 2009 - Volume 20 - Issue 3 - p 56-62 doi: 10.1097/MRM.0b013e328331ada5 Buy Metrics Abstract Inappropriate and inaccurate antimicrobial therapy can lead to adverse patient outcomes and also the development of antimicrobial resistance. Fluorescence in-situ hybridization using peptide nucleic acid (PNA FISH) uses fluorescent-labelled PNA probes in a highly sensitive and specific FISH assay targeting the species-specific ribosomal RNA in microbes. PNA FISH is suitable for routine examination in the clinical microbiology laboratory, and provides rapid results to guide effective and appropriate therapy within a time frame not possible using conventional microbiological methods. The present review highlights the PNA FISH technique for rapid diagnosis of infectious diseases. © 2009 Lippincott Williams & Wilkins, Inc.