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Reproducibility and Capacity to Detect Macular and Retinal Nerve Fiber Layer Thickness Alterations

Garcia-Martin, Elena PhD*,†; Pinilla, Isabel Prof†,‡; Sancho, Eva BA*; Almarcegui, Carmen MD§; Dolz, Isabel MD§; Rodriguez-Mena, Diego MD§; Fuertes, Isabel PhD*; Cuenca, Nicolas Prof

doi: 10.1097/IAE.0b013e318242b854
Original Study: PDF Only

Purpose To evaluate the ability of time-domain and Fourier-domain optical coherence tomographies (OCTs) to detect macular and retinal nerve fiber layer atrophies in retinitis pigmentosa (RP). To test the intrasession reproducibility using three OCT instruments (Stratus, Cirrus, and Spectralis).

Methods Eighty eyes of 80 subjects (40 RP patients and 40 healthy subjects) underwent a visual field examination, together with 3 macular scans and 3 optic disk evaluations by the same experienced examiner using 3 OCT instruments. Differences between healthy and RP eyes were compared. The relationship between measurements with each OCT instrument was evaluated. Repeatability was studied by intraclass correlation coefficients and coefficients of variation.

Results Macular and retinal nerve fiber layer atrophies were detected in RP patients for all OCT parameters. Macular and retinal nerve fiber layer thicknesses, as determined by the different OCTs, were correlated but significantly different (P < 0.05). Reproducibility was moderately high using Stratus, good using Cirrus and Spectralis, and excellent using the Tru-track technology of Spectralis. In RP eyes, measurements showed higher variability compared with healthy eyes.

Conclusion Differences in thickness measurements existed between OCT instruments, despite there being a high degree of correlation. Fourier-domain OCT can be considered a valid and repeatability technique to detect retinal nerve fiber layer atrophy in RP patients.

Time-domain and Fourier-domain optical coherence tomographies are useful to detect macular and retinal nerve fiber layer atrophies in retinitis pigmentosa patients compared with healthy subjects, but reproducibility is higher using Cirrus and Spectralis instruments than using Stratus.

*Department of Ophthalmology, Miguel Servet University Hospital, Zaragoza, Spain

Aragones Institute of Health Sciences, Zaragoza, Spain

Department of Ophthalmology, Lozano Blesa University Hospital, Zaragoza, Spain

§Department of Neurophysiology, Miguel Servet University Hospital, Zaragoza, Spain

Department of Physiology, Genetics and Microbiology, University of Alicante, Alicante, Spain.

Reprint requests: Elena Garcia-Martin, PhD, Hospital Universitario Miguel Servet, C/Padre Arrupe, Consultas externas de Oftalmología, 50009-Zaragoza, Spain; e-mail:

Supported in part by the Instituto de Salud Carlos III grant PI080976, FIS PS09/01854 (E.G-.M., I.P.) and RETICS RD07/0062/0012 (I.P., N.C.).

The authors report no financial or proprietary conflict of interest.

© 2019 by Ophthalmic Communications Society, Inc.