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Kilic Muftuoglu, Ilkay MD*; Bartsch, Dirk-Uwe PhD*; Barteselli, Giulio MD*,†; Gaber, Raouf MD*; Nezgoda, Joseph MD*; Freeman, William R. MD*

doi: 10.1097/IAE.0000000000001525
Original Study

Purpose: To compare the visualization of the epiretinal membrane (ERM) using multicolor imaging (MCI) (Heidelberg Engineering, Carlsbad, CA) and conventional white light flood color fundus photography (FP) (Topcon).

Methods: The paired images of patients with ERM who underwent same-day MCI and FP examinations were reviewed. Visibility of the ERM was graded using a scale (0: not visible, 1: barely visible, and 2: clearly visible) by masked readers, and surface folds were counted to quantify the membrane visibility for each method. Images from individual color channels in MCI (green, blue, and infrared) were also graded using the same method to further investigate MCI images.

Results: Forty-eight eyes of 42 patients were included. The average ERM visibility score was 1.8 ± 0.37 for MCI and 1.01 ± 0.63 for FP (P < 0.001). The number of the surface folds detected per quadrant was signifi8cantly higher in MCI than that in FP (6.79 ± 3.32 vs. 2.85 ± 2.81, P < 0.001). The ERM was graded with similar scores on the two modalities in 43.8% of the eyes; in 56.2%, the ERM was better visualized on MCI than that on FP. Conventional FP failed to detect ERM in 11.4% of eyes when the mean central retinal thickness was <413 microns. Analysis of laser color reflectance revealed that green reflectance provided better detection of surface folds (5.54 ± 2.12) compared to blue reflectance (4.2 ± 2.34) and infrared reflectance (1.2 ± 0.9).

Conclusion: Multicolor scanning laser imaging provides superior ERM detection and delineation of surface folds than conventional FP, primarily due to the green channel present in the combination-pseudocolor image in MCI.

Multicolor imaging provides a higher delineation of retinal folds related with the epiretinal membrane than conventional fundus photography.

*Department of Ophthalmology, Jacobs Retina Center, Shiley Eye Institute, University of California San Diego, La Jolla, California; and

Genentech Inc, South San Francisco, California.

Reprint requests: William R. Freeman, MD, University of California San Diego, Jacobs Retina Center, Shiley Eye Institute, 9415 Campus Point Drive, La Jolla, CA 92037; e-mail:

Supported in part by UCSD Vision Research Center Core Grant P30EY022589 (W.R.F.), NIH Grant EY016323 (D.-U.B.), and an unrestricted grant from Research to Prevent Blindness, NY (W.R.F.), The Scientific and Technological Research Center of Turkey (TUBITAK) (I.K.M.). The funding organizations had no role in the design or conduct of this research.

G. Barteselli is a full-time employee at Genentech Inc. The remaining authors have no conflicting interests to disclose.

© 2018 by Ophthalmic Communications Society, Inc.