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REPRODUCIBILITY OF VESSEL DENSITY MEASUREMENT WITH OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY IN EYES WITH AND WITHOUT RETINOPATHY

You, Qisheng MD, PhD*,†,§; Freeman, William R. MD*,†; Weinreb, Robert N. MD*,‡; Zangwill, Linda PhD*,‡; Manalastas, Patricia I. C. MD*,‡; Saunders, Luke J. PhD*,‡; Nudleman, Eric MD, PhD*,†

doi: 10.1097/IAE.0000000000001407
Original Study
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Purpose: To determine the intravisit and intervisit reproducibility of optical coherence tomography angiography measurements of macular vessel density in eyes with and without retinal diseases.

Methods: Fifteen healthy volunteers and 22 patients with retinal diseases underwent repeated optical coherence tomography angiography (Angiovue Imaging System, Optovue Inc) scans after pupil dilation on 2 separate visit days. For each visit day, the eyes were scanned twice. Vessel density defined as the proportion of vessel area with flowing blood over the total measurement area was calculated using Angiovue software. Intravisit and intervisit reproducibility were summarized as coefficient of variations and intraclass correlation coefficients were calculated from variance component models.

Results: The coefficient of variations representing the intravisit reproducibility of the superficial macular vessel density measurements for different quadrants on 3 mm × 3-mm scans varied from 2.1% to 4.9% and 3.4% to 6.8% for healthy and diseased eyes, respectively, and for the intervisit it was 2.9% to 5.1% and 4.0% to 6.8%, respectively. The coefficient of variations were lower in healthy eyes than in diseased eyes, lower for intravisit than for intervisit, lower on 3 mm × 3-mm scans than on 6 mm × 6-mm scans, and lower for paracentral subfields than for central subfield.

Conclusion: The evidence presented here demonstrates good reproducibility of optical coherence tomography angiography for measurement of superficial macula vessel density in both healthy eyes and eyes with diabetic retinopathy without diabetic macular edema.

The evidence presented here demonstrates good reproducibility of optical coherence tomography angiography for measurement of superficial macula vessel density in both healthy eyes and eyes with diabetic retinopathy without diabetic macular edema.

*Shiley Eye Institute, Department of Ophthalmology, University of California-San Diego, La Jolla, California;

Jacobs Retina Center, University of California-San Diego, La Jolla, California;

Hamilton Glaucoma Center, University of California-San Diego, California; and

§Beijing Institutute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Reprint requests: Eric Nudleman, MD, PhD, Shiley Eye Institute, 9415 Campus Point Drive, La Jolla, CA 92093-0946; email: eric.nudleman@gmail.com

Supported in part by UCSD Vision Research Center Core Grant P30EY022589, an unrestricted grant from Research to Prevent Blindness, NY (W.R.F.); ICO-Retina Research Foundation Helmerich Fellowship, Beijing Talents Fund (2015000021223ZK22) and National Natural Science Foundation of China (No. 81400422) (Q.S.Y.).

L. Zangwill: Carl Zeiss Meditec Inc, Heidelberg Engineering GmbH, Optovue Inc, Topcon Medical Systems Inc Quark (F); Carl Zeiss Meditec Inc, Optovue Inc (R). R. N. Weinreb: Alcon, Allergan, Bausch+Lomb, Forsight, Topcon (C); Heidelberg Engineering, Carl Zeiss Meditec, Genentech, Konan, National Eye Institute, Neurovision, Optovue, Quark, Tomey, Topcon (F); Carl Zeiss Meditec (R). E. Nudleman: Allergan (C), Visunex Medical Systems (C). The remaining authors have no conflicting interests to disclose.

© 2017 by Ophthalmic Communications Society, Inc.