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Rodrigues, Eduardo B. MD; Shiroma, Helio MD; Penha, Fernando M. MD; Maia, Mauricio MD; Moraes-Filho, Milton N. MD; Ferreira, Magno MD; Portella, Renata MD; Novais, Eduardo MD; Hagedorn, Nadine; Farah, Michel E. MD

doi: 10.1097/IAE.0000000000000078
Original Study

Purpose: To present the development and initial experience of a novel colored perfluorocarbon liquid (PFCL) in vitreoretinal surgery.

Methods: This was an experimental laboratory study and prospective human interventional study. F6H8 (Fluoron GmbH) was colored by adding 0.3 g/L blue anthraquinone dye. Subsequently, 20% colored F6H8 was prepared by mixing with perfluorooctane or perfluorodecalin (Fluoron GmbH). The novel product is not yet FDA approved for human application. In the laboratory, the colored PFCL was covered with 1) uncolored PFCL, 2) BSS, and 3) silicone oil. Cell toxicity was evaluated in L929 mouse fibroblasts using a growth inhibition assay. Porcine ex vivo eyes were evaluated after vitrectomy followed by intravitreal and subretinal colored PFCL infusion. A pilot, prospective, noncomparative interventional study was conducted in patients with retinal detachment with proliferative vitreoretinopathy (PVR).

Results: The density of the colored PFLC mixture was 1.664 g/cm3 for perfluorooctane and 1.802 g/cm3 for perfluorodecalin. There was no relevant cell growth inhibition with any concentration of colored PFCL tested. Experiments in pigs revealed that infusion of the colored PFCL caused neither staining of the internal limiting membrane nor intravitreal residual droplets. In the prospective study, 9 eyes (75%) underwent surgery for rhegmatogenous retinal detachment with at least grade C PVR. The colored PFCL enabled retinal break examination and detection of residual intravitreal droplets in all surgeries. There was no case of separation or leakage of the dye from the PFCL solution that could have caused unwanted staining of the vitreous or epiretinal surface.

Conclusion: The colored PFCL enabled intraoperative maneuvers such as endolaser use. In addition, removal of the colored PFCL was easily achieved at the end of surgery.

The authors present a pilot study of a colored perfluorocarbon liquid for vitreoretinal surgery obtained by mixing F6H8, a blue anthraquinone-dye and perfluorooctane/perfluorodecaline. Experiments in pigs revealed neither staining of internal limiting membrane nor intravitreal residual droplets. In humans, the colored liquid enabled intraoperative maneuvers in eyes with retinal detachment.

Vision Institute (IPEPO), Department of Ophthalmology, Federal University of São Paulo, São Paulo, Brazil.

Reprint requests: Eduardo B. Rodrigues, MD, Vision Institute (IPEPO), Department of Ophthalmology, Federal University of São Paulo, Rua Almirante Barroso 45, Jade 204, Florianópolis Brazil 88030-460; e-mail:

None of the authors have any financial/conflicting interests to disclose.

© 2014 by Ophthalmic Communications Society, Inc.