Comparative sequencing of the human CB1 cannabinoid receptor gene coding exon: no structural mutations in individuals exhibiting extreme responses to cannabis: PDF OnlyComparative sequencing of the human CB1 cannabinoid receptor gene coding exon: no structural mutations in individuals exhibiting extreme responses to cannabisHoehe, Margret R.a,b; Rinn, Thomasc; Flachmeier, Christinaa; Heere, Petraa; Kunert, Hanns J.c; Timmermann, Bernda,b; Köpke, Karlaa,b; Ehrenreich, HannelorecAuthor Information aGenome Research, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany;bGenProfile AG, Berlin, Germany;cDepartments of Psychiatry and Neurology, Georg August University & Max-Planck-Institute for Experimental Medicine, Göttingen, Germany Correspondence to Dr Margret R. Hoehe, Genome Research, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, D-13092 Berlin, Germany. E-mail:[email protected] Received 9 October 2000; accepted 20 December 2000 Psychiatric Genetics: December 2000 - Volume 10 - Issue 4 - p 173-177 Buy Abstract Rare but striking individual differences in responsiveness to cannabinoids have been observed that might involve mutations in the gene encoding the brain-expressed cannabinoid receptor. In a preliminary study, the human CB1 cannabinoid receptor coding region was comparatively sequenced in different groups of individuals: one group showed acute psychotic symptoms after cannabis intake, while another group did not develop any psychopathology after long-term heavy cannabis abuse. No evidence for structural mutations was obtained, which might provide some insight into the molecular basis of individually different responsiveness to cannabinoids. Comparison of CB1 cannabinoid receptor amino acid sequences between species substantiated evidence that the protein sequence is relatively well conserved. © 2000 Lippincott Williams & Wilkins, Inc.