RESEARCH PAPERS: PDF OnlyA contribution to genome-wide association studies: search for susceptibility loci for schizophrenia using DNA microsatellite markers on chromosomes 19, 20, 21 and 22Kitao, Yoshiea; Inada, Toshiyaa; Arinami, Tadaob; Hirotsu, Chihiroc; Aoki, Satoshic; lijima, Yoshimia; Yamauchi, Tadamitsud; Yagi, GoheieAuthor Information aNational Institute of Mental Health, National Center of Neurology and Psychiatry, Chiba, Japan;bDepartment of Medical Genetics, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan;cGraduate School of Engineering, University of Tokyo, Tokyo, Japan;dSakuragaoka Memorial Hospital, Institute of Social Welfare, Tokyo, Japan;eDepartment of Neuropsychiatry, Keio University, School of Medicine, Tokyo, Japan Correspondence to Toshiya Inada, National Institute of Mental Health, National Center of Neurology and Psychiatry, 1-7-3, Kohnodai, Ichikawa, Chiba 272-0827, Japan. E-mail:[email protected] Received 30 October 2000; accepted 3 November 2000 Psychiatric Genetics: September 2000 - Volume 10 - Issue 3 - p 139-143 Buy Abstract As an initial step for genome-wide association studies, we sought an association between schizophrenia and 34 microsatellite markers on chromosomes 19, 20, 21 and 22 by a case-control design. The samples examined for an association were 168 schizophrenic patients and 146 control subjects in the Japanese population. The allele distribution of the 34 loci differed significantly between Japanese and French populations. Significant deviation from the Hardy-Weinberg equilibrium was observed at D19S209 and D21S1256 in the control subjects. Case-control comparisons of the initial screening revealed a significant difference in allele frequency at D20S95 and a trend of difference at D20S118. To confirm these possible associations, additional samples consisting of 110 schizophrenic patients and 116 control subjects were examined, and an association between D20S95 and schizophrenia was confirmed (correctedPvalue after Bonferroni correction, 0.00035). D20S95 is located close to the gene (CHGB) encoding chromogranin B. These findings suggest that CHGB could be an important candidate gene involved in the development of schizophrenia. © 2000 Lippincott Williams & Wilkins, Inc.