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Cosmetic: Original Article

Deep Layer Radiofrequency Thermo-coagulative Technology for Cervicofacial Contouring: Sonographic and Clinical Results

Cohen, Sarit MD*,†; Dominsky, Omri MD*,†; Artzi, Ofir MD†,‡; Dayan, Erez MD§; Eckstein, Joseph MD

Author Information
Plastic and Reconstructive Surgery - Global Open: December 2020 - Volume 8 - Issue 12 - p e3286
doi: 10.1097/GOX.0000000000003286
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Lower face and cervical aging refers to complex additive and subtractive changes that occur throughout all facial layers.1 Skin redundancy and laxity, subcutaneous lipodystrophy coupled with attenuation of the retaining ligaments and the intervening fibrous septa ultimately give rise to lower face and neck aging stigmata.

Energy-based nonsurgical modalities for skin tightening include lasers, focused ultrasound, and radiofrequency. Radiofrequency (RF) has become the increasingly more popular modality to date. It thermally induces collagen contraction and its subsequent remodeling.2 The resultant dermal tightening has been substantiated histologically and clinically by many researchers.2–5 Other facial layers deeper to the dermis, including the fibrous septa that compartmentalize the subcutaneous fat (layer 2),6 the superficial muscular aponeurotic system (SMAS) (layer 3)7,8 and the retaining ligaments (layer 4), also contain collagen.1,9

Recently, the new generation of minimally-invasive, temperature controlled, bipolar RF-based technology was approved by the FDA for electrocoagulation and hemostasis. Literature review reveals only 2 case series of cervicofacial contouring using RF thermocoagulation (RFTC).10,11 The esthetic outcome in these studies was based on a patient satisfaction survey with little objective data. One study also included photographic evaluation by independent physicians.11

The aim of the present pilot study was to present a deep layer radiofrequency thermo-coagulative energy employment technique and to objectively evaluate its efficacy both quantitatively and qualitatively.


Study Design

This prospective, 1-arm cohort study was performed in a single medical center, from June 2017 to June 2018. The study was approved by the institutional review board of Assaf Harofeh Medical Center and was conducted in accordance with the ethical principles laid down in the Declaration of Helsinki.

Patients and Settings

Ten healthy women (including smokers) seeking for lower face and neck contouring were recruited to this study. Written informed consent was obtained from all participants. Inclusion criteria were patients with mild-to-moderate sagging, submental fat, and mild-to-moderate jowling (Baker’s classification type 1 and 2).12 According to this classification, type 1 patients have slight cervical skin laxity with submental fat and early jowls, whereas type 2 patients have moderate cervical skin laxity, moderate jowls, and submental fat.

Subjects were excluded from the study if they had a pacemaker or internal defibrillator, or any other active electrical implant anywhere in the body, superficial permanent implant in the treatment area, or had undergone cosmetic procedures in the face and neck in the previous 6 months. Also excluded were those who had scars or infections in the target area, significant facial asymmetry, were receiving anticoagulant or immunosuppressive treatment, had autoimmune diseases, a history of massive weight loss, or those who were pregnant or breastfeeding. Patients with unrealistic expectations were excluded as well.

Each patient had 1 treatment session. Ultrasound examination of the lower face and neck were performed before and at 6 weeks postoperatively. Standardized photographs were taken before and at 12 months postoperatively. Follow-up visits were scheduled at 2, 6 weeks and at 12 months postoperatively.

The Device

The RFTC device is a bipolar, temperature-controlled, radiofrequency-technology consisting of a hand piece powered by the InMode platform. The hand piece consists of an internal solid rod electrode (cannula) of 10-cm long and 1.3-mm diameter and an external electrode, connected by a hand grip. The internal electrode is a bullet-shaped plastic dissector, coated at its tip to prevent accidental overheating. The external electrode has a relatively large diameter aimed to disperse energy near the skin’s surface and prevent overheating. RF density is higher around the smaller internal electrode and the RF energy is transmitted in a gradient, tear-drop pattern from the tip of the internal cannula to the external electrode. Internal RF energy delivery thermally coagulates the intervening collagenous, vascular, and adipose tissue and liquefies fat. External RF delivery thermally induces non-coagulative dermal collagen stimulation.

Both external and internal electrodes have embedded temperature sensors that monitor the respective temperatures. The internal electrode cut-off temperature range is 50–70°C and the external electrode cut-off temperature range is 35–43°C. When the predetermined cut-off temperature is reached, RF power is automatically switched off. Additional internally monitored safety measures include the rate of temperature rise, tissue impedance, and audible feedback. Rapid increase in temperature or too precipitous changes of impedance automatically “cuts off” RF power. Treatment depth is controlled by adjusting the distance between the 2 electrodes.

Preoperative Markings and Anesthesia

Preoperative marking included 5 treatment zones: a triangular zone above the mandibular border, bilaterally, and the neck itself divided into 3 triangular zones (2 lateral and 1 central). A line extending 0.5 cm above and below the mandibular border demarcates the facial and cervical treatment zones, respectively. The superior border of the treatment zone is marked by a line drawn from the ear lobule toward the lower 3rd of the nasolabial fold. The medial border above the mandible extends up to a vertical line descending from the lateral orbital rim. The inferior border extends up to the 2nd transverse neck crease (Fig. 1).

Modified Klein tumescent solution (50 ml of lidocaine 1%, 50 ml of 0.9% saline solution and 0.5 mg of epinephrine, 1:1000) is infiltrated using a 14-gauge infiltration cannula through 3 access incision ports: 2 infra-lobular and 1 submental (Fig. 1). Each treatment zone is infiltrated with 20 cm3 of tumescent solution to achieve sufficient turgor. Sterile ultrasound gel is applied to improve RF coupling and electrode gliding. Cut-off temperature settings are 39°C externally and 69°C internally.

Surgical Technique

The radiofrequency thermo-coagulative hand piece is introduced through the access incisions, employing bipolar, temperature-controlled radiofrequency-technology to the face and neck. The internal cannula is tunneled through the subcutaneous layer to the sub-SMAS plane, while the external electrode runs in tandem along the skin surface. RF energy is delivered at 20 watts in a retrograde linear threading fashion, until the predetermined internal cut off temperature is reached. Successive passes in a fanning manner ensure that all pre-marked treatment zone is encompassed. Electrical current flows from the cannula tip to the external electrode, creating confined controlled energy deposition and uniform heating. The internal cannula coagulates and liquefies subcutaneous fat and simultaneously contracts the deep layers of collagen-containing tissues. The amount of RF energy delivered for each zone is 1.5–2 KJ.

Postoperative Care

Patients were instructed to wear a facial strap daily for the first 3 days and to come for lymphatic massage at 1 week and 2 weeks postoperatively.

Outcome Measures

To objectively evaluate the efficacy of the RFTC technique, the thickness and echogenicity of layers 1–5 at 3 anatomic locations were sonographically measured and recorded at base-line and at 6 weeks postoperatively:

  1. Lower face: The transducer (GE 12L-RS linear probe) was located along a vertical line, descending from the lateral orbital rim in front of the masseter muscle.
  2. Lateral neck: Along the lateral border of the platysma, with the cranial portion of the probe placed one finger breadth below the angle of the mandible, corresponding to the location of the cervical retaining ligaments.13
  3. Submental region: The transducer was located horizontally halfway between the menton and hyoid bone.

These anatomical landmarks (ie, lateral orbital rim, mandibular angle, menton and hyoid bone) were chosen as reference points to avoid potential measurement bias.

Ultrasonic image acquisition and measurements were conducted by a board-certified radiologist using a GE Voluson e ultrasound machine. The system offers 4D image quality, live dual (B/BC(mode, and automated functional imaging. All images were obtained from the same 3 anatomical locations. The thickness of facial layers 1–5 of each of the 3 locations was measured in centimeters before and at 6 weeks postoperatively.

The echogenicity of facial layers (1–5) was analyzed based on the number of pixels counted from the ultrasound images obtained and processed by image analysis, using the Matlab-based application (The Mathworks, Natick, Mass.). This validated method measures echogenicity on a numerical scale extending from 0 (non-echogenic) to 255 (maximal echogenicity).14 High echogenic pixels (HEPs), defined as 170–255, were recorded for each of the 3 locations before and at 6 weeks postoperatively. An increase in the number of HEPs correlates with an increase in echogenicity.

In terms of echogenicity, hyperechoic signals reflect the collagen fibers in facial tissues, whereas the hypoechoic ones originate from the extracellular matrix that lies between the collagen fibers and the subcutaneous fat. Thus, post-operative increase in echogenicity in layers 1–5 reflects collagenesis induced by the RFTC device. Twelve sets of data were collected: pre- and post-operative thickness, and echogenicity along the above-mentioned 3 anatomic locations.

Clinical outcome/efficacy was evaluated by 2 independent physicians (a plastic surgeon and a dermatologist) who rated baseline and 12-months postoperative status using a validated 5-point assessment scale of the lower face15 and the Merz jawline scale.16,17 The validated 5-point assessment scale was rated as follows: 1—little or no improvement (0%–10%); 2—noticeable improvement (10%–25%); 3—fair improvement (25%–50%); 4—good improvement (50%–75%); and 5—excellent improvement (>75%). The Merz jawline scale was rated as follows: 0—No sagging; 1—mild sagging; 2—moderate sagging; 3—severe sagging; and 4—very severe sagging. Clinical outcome at 12 months was also evaluated by subjects on a scale of 1–4, with 1 being “not satisfied” and 4 being “very satisfied” with the outcome of the treatment. Patients’ tolerance was evaluated using a scale of 1–4, with 1 representing poor tolerance, and 4 representing excellent tolerance. Adverse effects were also documented at follow-up visits

Statistical Analysis

Pre- and post-treatment echogenicity and thickness were compared using the Wilcoxon paired test. The percent of change was calculated for each patient at each area as follows:

The average percent of change was presented per measure and location. Significance level was defined as α = 0.05. Statistical analyses were performed with SPSS 25.0.


All patients completed the study. Mean age was 60.2 years (range, 52–76 years). Thickness and echogenicity measurements are depicted in Table 1.

Table 1. - Echogenicity and Thickness Measurements of Layers 1–5
Lateral Neck Lower Face Submental
Echogenicity(HEPs) Pre 4.5 ± 1.7 5.6 ± 2.2 3.2 ± 1.4
Post 10.6 ± 7.3 9.5 ± 5.7 5.0 ± 2.8
Percent change 149.3% ± 178.7% 77.7% ± 91.7% 60.3% ± 68.3%
P 0.01 0.02 0.01
Thickness (cm) Pre 1.7 ± 0.2 1.4 ± 0.1 1.8 ± 0.2
Post 1.5 ± 0.3 1.3 ± 0.1 1.5 ± 0.2
Percent change –16.2% ± 10.3% –5.8% ± 3.5% –18.7% ± 10.3%
P 0.01 0.01 0.01

Following RFTC, there was an increase in the HEPs counted in all sites. The percentage increase per site was 149% in the lateral neck region, 78% in the lower face area, and 60% in the submental region. The differences between pre- and post-treatment were statistically significant (P = 0.01–0.02). These significant increases in echogenicity reflect an increase in the collagen content in layers 1–5.

A postoperative global decrease in thickness was noted. The percentage thickness decrease per site was 16% for the lateral neck, 6% for lower face, and 19% for the submental region (P = 0.01). These reductions reflect the tightening effect due to reduced fatty content, collagenous tissue contraction and rearrangement of collagen fibers.

The average physicians’ improvement in lower face contour was 3.8 (indicating 50-75% improvement). The average physicians’ Merz jawline scale was improved from 2.85 at baseline to 1.05 at 12 months posttreatment. The physicians’ correlations of all measurements were significant and ranged between medium to strong (0.60 < R < 0.94), indicating that results were reliable and could be averaged. Patients’ tolerance was 3.3 and patient satisfaction was 3.5. Side effects were minimal. Patient characteristics and treatment outcomes are shown in Table 2. Case presentations and selected ultrasound findings demonstrating thickness and echogenicity are presented in Figures 2–6.

Table 2. - Patient Characteristics and Treatment Outcomes in 10 Women Who Underwent Deep Layer RF Cervicofacial Contouring
Pt. no. Initials Age Baseline 12 months after procedure Improvement Satisfaction Tolerance Adverse Effects
P1 P2 Average P1 P2 Average P1 P2 Average
1 HA 52 3 3 3 2 1 1.5 3 3 3 3 4 NA
2 HG 76 4 3 3.5 2 1 1.5 4 3 3.5 4 3 NA
3 MA 63 3 4 3.5 1 1 1 3 3 3 3 4 NA
4 YL 47 1 2 1.5 1 1 1 4 5 4.5 4 3 NA
5 NBA 66 4 3 3.5 1 1 1 5 4 4.5 3 3 NA
6 MC 69 2 2 2 1 1 1 4 5 4.5 4 3 NA
7 DA 59 3 4 3.5 1 1 1 4 3 3.5 2 3 Edema
8 AZ 61 2 1 1.5 1 0 0.5 4 4 4 4 3 NA
9 YL 52 3 4 3.5 1 1 1 3 4 3.5 4 3 NA
10 SH 57 3 3 3 1 1 1 4 4 4 4 4 NA
Average 60.2 2.85 1.05 3.8 3.5 3.3
NA, not applicable.

Fig. 1.
Fig. 1.:
Preoperative markings. The lower face and neck are divided into 5 triangular treatment zones: a triangular zone above the mandibular border, bilaterally, and the neck itself divided into 3 triangular zones (2 lateral and 1 central). The access incision ports (marked in color) include an infralobular, bilateral, and submental.
Fig. 2.
Fig. 2.:
A 65-year-old patient before (A) and at 12 months following treatment (B). Note the improvement in jawline definition and cervicomental angle.
Fig. 3.
Fig. 3.:
US image of the lateral neck before (A) and at 6 weeks postoperatively (B), demonstrating a profound contraction and a marked decrease in facial layers 1–5 thickness (from 1.78 cm to 1.2 cm). Insets show the marked increase in echogenicity of facial layers. The number of HEPS counted by image analysis was 4.2564 before (C) and 10.4887 postoperatively (D), reflecting collagenesis induced by the RFTC device.
Fig. 4.
Fig. 4.:
US image of the submental region before (A) and at 6 weeks postoperatively (B), demonstrating a marked decrease in facial layers thickness (from 1.98 cm to 1.35 cm). Significant lipolysis, collagenous tissue contraction, and rearrangement are visibly appreciable. Note: the liquefied fat was replaced by neocollagenesis. The number of HEPS was 4.951 before (C) and 5.4249 postoperatively (D).
Fig. 5.
Fig. 5.:
A 56-year-old patient before (A) and at 12 months following treatment (B).
Fig. 6.
Fig. 6.:
US image of the lower midcheek before (A) and at 6 weeks postoperatively (B), demonstrating more densely compacted organized collagenous containing tissue, an overall decrease in thickness (1.34–1.2 cm following treatment). The number of HEPs was 8.2058 before (C) and 22.1062 postoperatively (D).


Facial aging is a multilayered process, affecting collagen-containing tissues in layers 1–4 (dermis, fibrous septa, SMAS, and the retaining ligaments, respectively) as well as fatty tissue. Age-dependent changes of the collagen-containing facial tissues (stretching, fragmentation, and attenuation) and of the facial fat compartments (inferior migration and inferior volume shift within the compartments) contribute to the stigmata of facial aging.

Current nonsurgical modalities for facial contouring include lasers, ultrasound, and RF. RF-based technologies have been revolutionized from the thermally-induced collagen contraction residing only in the dermis,3–5 to contraction of the fibroseptal network. This is a paradigm shift from a 2-dimensional horizontal tightening (aimed to improve skin laxity)2,5,18 to a 3-dimensional tissue tightening (which involves the deep layers of collagen-containing tissues).19 The latest RF technology is the temperature-controlled RF energy deposition, affecting the collagenous as well as adipose tissues. This RF-assisted liposuction has been practiced for body contouring,19–21 and the reported soft tissue contraction was superior to other liposuction modalities: up to 47%19 compared with 17%22 for laser-assisted lipolysis at 6 months and 6% for suction-assisted lipoplasty.23 The resultant tissue tightening and lipolysis might be beneficial for lower face and cervical contouring as well.

Our study is the first prospective cohort of patients treated by RFTC device to the sub-SMAS layer for lower face and cervical contouring. It is also the first cohort study in which the RF effect was documented both quantitatively and qualitatively by pre- and post-operative ultrasound measurements of the facial layers 1–5. RF energy was delivered to the sub-SMAS layer in a bipolar manner, creating a controlled, confined thermal effect to the intervening layers: SMAS, subcutaneous tissue, and the reticular dermis. The sub-SMAS (layer 4) is essentially an avascular potential space that contains soft tissues spaces, retaining ligaments and facial nerve branches passing from deep to superficial, in relation to defined landmarks. The facial nerve branches remain deep to the deep fascia (layer 5) in the lateral face—ie, lateral to vertical line descending from the lateral orbital rim. Medial to this line, in close association with the retaining ligaments, these branches traverse layer 4 to innervate the underside of the mimeic muscles.1 This anatomy was verified by multiple cadaver dissections.24,25 The upper and lower buccal trunks traverse layer 4 as they approach the anterior border of the masseter, in association with the upper and lower key masseteric ligaments, respectively.1,24 The marginal mandibular branch traverses layer 4 crossing the facial vessels 3.1 mm superior to the lower mandibular border.25 Hence, treatment in sub-SMAS plane in the lateral face, and at a distance of 0.5 cm from the lower mandibular border, as presented in our technique, is safe and void of untoward facial nerve injury. The safety of dissection in the sub-SMAS plane has also been well documented for deep-plane rhytidectomies, with rates of temporary facial nerve neuropraxia of 1% and no permanent facial nerve injury. This is within the 2.1% reported incidence of temporary neuropraxia from a survey of more than 12,000 facelifts.26 Multi-layer volumetric heating and the reduced risk of thermal injury of the superficial facial layers (eg, nodules, tissues hardening) are other putative advantages of the sub-SMAS plane.

We herein report clinical and objective sonographic responses of 10 patients following RFTC treatment. Most patients experienced significant clinical improvement and were highly satisfied with the results. All patients exhibited very good tolerance to the device, reporting minimal pain, discomfort, and swelling, which subsided shortly after treatment. A significant increase in echogenicity of facial layers 1–5 was shown: 149% in lateral neck, 78% in lower face, and 60% in submental. We postulate that this collagenesis is attributed not only to the thermally induced collagen remodeling of the deep layer collagen-containing tissues, but also to an active fibroblastic reaction replacing the liquefied fat. The sonographic images demonstrated a visibly appreciable more organized, compact, densely arranged layers—ie, reflecting collagen rearrangement. These changes may also count for the increase in echogenicity and decreased thickness measurements. We also noted a significant decrease in thickness of facial layers 1–5. These data exhibit substantial tissue tightening, reflecting a significant thermally-mediated contraction of the collagenous containing tissue, as well as liquefaction and lipolysis.

The present study also provides compelling evidence for the dual functionality of invasive RF-based technology: the tightening effect of the collagen-containing facial tissues coupled with the RF-assisted lipolysis. Although this study is limited by the small sample size, 12 sets of objective data were collected for each patient, and the treatment efficacy was evaluated objectively by quantitative and qualitative sonographic measurements in 3 locations. The relatively short-term sonographic follow-up is another potential limitation. Yet, it is well known that RF-induced soft tissue contraction and tightening continue to improve with time. This is in line with our clinical observation. Further multicenter studies with a longer follow-up are warranted.

In conclusion, deep layer treatment using RFTC opens a new horizon for nonsurgical cervicofacial contouring. It allows multilayer collagenous tissue contraction, profound tightening, and visibly appreciable lipolysis. The technique presented herein is safe and effective, and serves as an alternative for surgery in selected patients.


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Copyright © 2020 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons.