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Evaluation of Lactate in Whole Blood by GEM Premier 3000: Comparison to Lactate by the Omni (Whole Blood) and Vitros (Plasma) Analyzers

Toffaletti, John G. PhD; McDonnell, Elizabeth H. BS, MT(ASCP); Dean, L. Ray MT(AMT)

Point of Care: The Journal of Near-Patient Testing & Technology: December 2002 - Volume 1 - Issue 4 - p 229–232
Article

The authors evaluated a new method for measuring lactate in undiluted whole blood by direct amperometry (GEM Premier 3000 (GEM), Instrumentation Laboratory) by comparison to results from two other analyzers: Omni 9, (Roche Diagnostics) (whole blood; direct amperometry); and Vitros 950, (Johnson & Johnson Clinical Diagnostics, Inc) (plasma; enzymatic conversion on multilayered dry slide, detection by light reflectance). While all methods showed good agreement, comparison of 103 lactate results showed clear differences among the methods, most likely related to calibration set-points established by the manufacturers. Plasma lactate results the Vitros typically fell between results on whole blood by the other methods. Lactate by the Omni was higher at all concentrations. While GEM lactate results up to ˜2.5 mmol/L were very close to the Vitros results (average difference 0.06 mmol/L), above 2.5 mmol/L, lactate became proportionately lower (average difference = −0.68 mmol/L). The authors conclude the GEM's analytical performance is equivalent to that of other analyzers, and has features suitable for near-patient testing.

From the Blood Gas Laboratory and Department of Pathology, Duke University Medical Center, Durham, North Carolina.

This study was financially supported by Instrumentation Laboratory.

Address correspondence and reprint requests to John G. Toffaletti, PhD, PO Box 3015, Duke University Medical Center, Durham, NC 27710 (email:toffa002@mc.duke.edu).

© 2002 Lippincott Williams & Wilkins, Inc.