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Rapid, Ultrasensitive Isolation of Pathogenic Candida Species Directly From Whole Human Blood

Clarizia, Lisa-Jo PhD; Standridge, Douglas BS; Davila, Michael BS; Springer, Stephen BS; Bauer, Ruth MS; Vandervest, Jaclyn BS; Barr, Alexandra MS; Ung, Thearith PhD; Anthony, Spencer MS; Otero, Rosa BA; Norvell, Meghan PhD; Macemon, James MAS; Esch, Victor PhD

Point of Care: The Journal of Near-Patient Testing & Technology: March 2016 - Volume 15 - Issue 1 - p 4–6
doi: 10.1097/POC.0000000000000089
Original Articles
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Candidemia is a type of bloodstream infection generally associated with an extremely high mortality rate. Delay in accurate diagnosis and administration of appropriate therapy in patients with candidemia is directly associated with poor clinical outcomes. The current gold standard diagnostic for candidemia, blood culture, requires extended incubation periods up to 96 hours before cultures turn positive, with additional time required for speciation and characterization of antifungal sensitivity. Although new identification tools permit rapid identification of organisms isolated from positive blood culture bottles, time to diagnosis is still intimately linked with blood culture time to positivity. There is therefore a compelling need for a diagnostic tool which rapidly isolates the causative agents of candidemia directly from whole blood, permitting accelerated identification and characterization of these organisms, allowing clinicians to initiate appropriate therapies as rapidly as possible. We report on the development of a culture-free pathogen capture system based on immunomagnetic capture of fungal pathogens directly from whole patient blood in less than 60 minutes. This pathogen capture system was successfully used to isolate clinically relevant concentrations of pathogenic Candida species spiked into healthy donor blood. Results from these spike-and-recovery studies demonstrate that this pathogen capture system can recover Candida at clinically relevant levels directly from human blood.

From the nanoMR, Inc, Albuquerque, NM.

Reprints: Lisa-Jo Clarizia, PhD, 5749 Midway Park Blvd NE, Albuquerque, NM 87109. E-mail: lclarizia@nanomr.com.

The authors declare no conflict of interest.

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