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Novel POC Analysis for Determination of Total and 5-Part Differential WBC Counts Among a US Population, in Comparison to Beckman Coulter LH750

Hockum, Sandra MSc*; Johnsson, Elin BSc*; Reed, Jeremy MBA, BSc, MT(ASCP)

doi: 10.1097/POC.0000000000000007
Original Articles

The HemoCue WBC DIFF system uses a novel imaging technology to classify total and differential white blood cell (WBC) counts. To evaluate possible effects of ethnical and demographical differences among samples from a US population for this novel imaging technology, the HemoCue WBC DIFF system was compared to the Beckman Coulter LH750, which uses the Coulter Principle to classify differential WBC count. Excellent results were observed on WBC, neutrophils, and lymphocytes with a correlation r = 0.988; y = 1.01 · x − 0.22, r = 0.984; y = 0.98 · x + 0.17, and r = 0.968; y = 0.91 · x + 0.13, respectively. For monocytes, the HemoCue WBC DIFF system measured slightly lower and the correlation for eosinophils was overall good. No impact due to technology could be seen in this study when HemoCue WBC DIFF was compared to one of the state-of-the-art laboratory cell counters.

From the *HemoCue AB, Ängelholm, Sweden and †Quest Diagnostics, Baltimore, MD.

Reprints: Sandra Hockum, MSc, HemoCue AB, Box 1204, SE-262 23 Ängelholm, Sweden. E-mail:

Johnsson, E. and Hockum, S., employees at HemoCue AB, performed the study. Reed, J. is employed at Quest Diagnostics of which HemoCue AB is a subsidiary. No funds were given, and no other conflicts of interest were identified.

© 2014 by Lippincott Williams & Wilkins