In this frostbite model, the wounds were circular and generally corresponded in size and shape to the magnets placed for wound creation. Even the largest wound did not extend beyond the margin of the contact site with the magnet. Therefore, it was considered that the wound area was determined by the severity of peripheral zone injury, rather than central injury. Accordingly, the current results suggest that extract of Ginkgo biloba 761 is more likely to be effective at the border zone of frostbite where reperfusion injury may have a greater impact than at the central zone where direct damage caused by freezing is more important. The histologic findings supported this concept, revealing numerous cells with lipid peroxidation in group I versus very few in group II. These cells presumably underwent necrosis and phagocytosis within a few days, because no stained cells were found on days 3 and 5 in either group.
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