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In Vitro Differentiation of Human Processed Lipoaspirate Cells into Early Neural Progenitors

Ashjian, Peter H. M.D.; Elbarbary, Amir S. M.D.; Edmonds, Brian Ph.D.; DeUgarte, Daniel M.D.; Zhu, Min M.D.; Zuk, Patricia A. Ph.D.; Lorenz, Peter H. M.D.; Benhaim, Prosper M.D.; Hedrick, Marc H. M.D.

Plastic and Reconstructive Surgery: May 2003 - Volume 111 - Issue 6 - p 1922–1931
doi: 10.1097/01.PRS.0000055043.62589.05
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Human processed lipoaspirate (PLA) cells are multipotent stem cells, capable of differentiating into multiple mesenchymal lineages (bone, cartilage, fat, and muscle). To date, differentiation to nonmesodermal fates has not been reported. This study demonstrates that PLA cells can be induced to differentiate into early neural progenitors, which are of an ectodermal origin. Undifferentiated cultures of human PLA cells expressed markers characteristic of neural cells such as neuron-specific enolase (NSE), vimentin, and neuron-specific nuclear protein (NeuN). After 2 weeks of treatment of PLA cells with isobutylmethylxanthine, indomethacin, and insulin, about 20 to 25 percent of the cells differentiated into cells with typical neural morphologic characteristics, accompanied by increased expression of NSE, vimentin, and the nervegrowth factor receptor trk-A. However, induced PLA cells did not express the mature neuronal marker, MAP, or the mature astrocyte marker, GFAP. It was also found that neurally induced PLA cells displayed a delayed-rectifier type K+ current (an early developmental ion channel) concomitantly with morphologic changes and increased expression of neural-specific markers. The authors concluded that human PLA cells might have the potential to differentiate in vitro into cells that represent early progenitors of neurons and/or glia. (Plast. Reconstr. Surg. 111: 1922, 2003.)

Los Angeles, Calif.

From the Laboratory for Regenerative Bioengineering and Repair, Departments of Surgery and Orthopedics, University of California, Los Angeles, School of Medicine. Received for publication March 12, 2002; revised July 25, 2002.

Presented at the 70th Annual Scientific Meeting of the American Society of Plastic Surgeons in Orlando, Florida, November 3 through 7, 2001; the 46th Annual Meeting of the Plastic Surgery Research Council in Milwaukee, Wisconsin, June 9 through 12, 2001; and the 2001 Surgical Forum of the American College of Surgeons in New Orleans, Louisiana, October 7 through 12, 2001.

Drs. Hedrick, Benhaim, and Lorenz have a financial interest in technology related to this scientific study.

Marc H. Hedrick, M.D. Division of Plastic and Reconstructive Surgery UCLA School of Medicine 64-140 Center for Health Sciences 10833 Le Conte Avenue Los Angeles, Calif. 90095

©2003American Society of Plastic Surgeons