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Synergistic Effect of Adipose-Derived Stem Cells and Fat Graft on Wrinkles in Aged Mice

Kim, Kikap, M.D., M.S.; Fan, Yingfang, M.D., Ph.D.; Lin, Guang, M.D., M.S.; Park, Yoon Kyung, Ph.D.; Pak, Chang Sik, M.D.; Jeong, Jae Hoon, M.D., Ph.D.; Kim, Sukwha, M.D., Ph.D.

Plastic and Reconstructive Surgery: June 2019 - Volume 143 - Issue 6 - p 1637–1646
doi: 10.1097/PRS.0000000000005625
Experimental
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Background: The authors investigated the synergistic effects of adipose-derived stem cells and fat graft on skin wrinkles in a nude mouse model of chronologic aging.

Methods: After 50 weeks of chronologic aging, 44 female BALB/c nude mice were classified into four groups: (1) negative control, (2) mice injected subcutaneously with fat on the back skin (0.5 cm3), (3) mice injected with adipose-derived stem cells (1 × 105 cells in 0.5 cm3 Hanks balanced salt solution), and (4) mice injected with both fat (0.5 cm3) and adipose-derived stem cells (1 × 105 cells in 0.5 cm3 Hanks balanced salt solution). The degree of wrinkling was evaluated using replica analysis, and skin biopsies were performed after 4 weeks. The dermal thickness and density of collagen were determined. Type I procollagen and matrix metalloproteinase levels were determined using real-time polymerase chain reaction and Western blot analysis. Tropoelastin, fibrillin-1, and CD31 levels were evaluated using immunohistochemistry.

Results: Based on the total wrinkle area, there was significant wrinkle reduction in the fat-treated and adipose-derived stem cell with fat–treated groups. Type I procollagen mRNA and collagen levels were significantly higher in the adipose-derived stem cell with fat–treated group than in the adipose-derived stem cell–treated and the fat-treated groups. In addition, the adipose-derived stem cells with fat graft group exhibited significantly higher CD31 expression level than the adipose-derived stem cell–treated and the fat-treated groups.

Conclusion: Both adipose-derived stem cells and fat graft have a wrinkle-reducing effect and synergistically affect collagen synthesis and neovascularization.

Seoul and Seongnam, Republic of Korea

From the Department of Plastic and Reconstructive Surgery, Seoul National University College of Medicine; the Department of Pediatric Plastic and Reconstructive Surgery, Seoul National University Children’s Hospital; and the Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital.

Received for publication May 17, 2018; accepted October 25, 2018.

Dr. Jae Hoon Jeong and Dr. Sukwha Kim contributed equally to this article as corresponding authors.

Disclosure:The authors have no financial interest to declare in relation to the content of this article.

Sukwha Kim, M.D., Ph.D., Seoul National University College of Medicine, 101, Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea, kimsw@snu.ac.kr

Jae Hoon Jeong, M.D., Ph.D., Seoul National University Bundang Hospital, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam 13620, Republic of Korea, psdrj2h@gmail.com

©2019American Society of Plastic Surgeons