Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

Extracellular Vesicles from Human Adipose-Derived Stem Cells for the Improvement of Angiogenesis and Fat-Grafting Application

Mou, Shan M.D.; Zhou, Muran M.D.; Li, Yuan M.D.; Wang, Jiecong M.D., Ph.D.; Yuan, Quan M.D., Ph.D.; Xiao, Peng M.D., Ph.D.; Sun, Jiaming M.D., Ph.D.; Wang, Zhenxing M.D., Ph.D.

Plastic and Reconstructive Surgery: October 2019 - Volume 144 - Issue 4 - p 869-880
doi: 10.1097/PRS.0000000000006046
Experimental
Buy
SDC
Discussion

Background: The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis that are closely related to unsatisfactory vascularization. Extracellular vesicles are key components of the cell secretome, which can mirror the functional and molecular characteristics of their parental cells. Growing evidence has revealed that adipose-derived mesenchymal stem cells have the ability to enhance vascularization, which is partly ascribed to extracellular vesicles. The authors evaluated whether adipose-derived mesenchymal stem cell–derived extracellular vesicles improved vascularization of fat grafts and increased their retention rate.

Methods: To test the angiogenesis ability of adipose-derived mesenchymal stem cell–derived extracellular vesicles, they were isolated from the supernatant of cultured human adipose-derived mesenchymal stem cells and incubated with human umbilical vein endothelial cells in vitro. Then, the vesicles were co-transplanted with fat into nude mice subcutaneously. Three months after transplantation, the retention rate and inflammatory reaction of the grafts were analyzed by histologic assay.

Results: The experimental group could significantly promote migration and tube formation at the concentration of 20 μg/ml. At 3 months after transplantation, the volume of the experimental group (0.12 ± 0.03 mm3) was larger compared with the blank group (0.05 ± 0.01 mm3). Histology and immunohistology results demonstrated significantly fewer cysts and vacuoles, less fibrosis, and more neovessels in the extracelluar vesicle group.

Conclusions: The authors co-transplanted adipose-derived mesenchymal stem cell–derived extracellular vesicles with fat into a nude mouse model and found that the vesicles improved volume retention by enhancing vascularization and regulating the inflammatory response.

Wuhan, People’s Republic of China

From the Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology.

Received for publication June 17, 2018; accepted February 14, 2019.

Disclosure:The authors have no financial interest to declare in relationto the content of this article.

Related digital media are available in the full-text version of the article on www.PRSJournal.com.

Zhenxing Wang, M.D., Ph.D., Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China, benjamin.wzx@163.com

Jiaming Sun, M.D., Ph.D., Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China, sunjm1592@sina.com

Copyright © 2019 by the American Society of Plastic Surgeons