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Light-Activated Sealing of Nerve Graft Coaptation Sites Improves Outcome following Large Gap Peripheral Nerve Injury

Fairbairn, Neil G. M.D.; Ng-Glazier, Joanna M.D.; Meppelink, Amanda M. B.S.; Randolph, Mark A. M.A.S.; Valerio, Ian L. M.D.; Fleming, Mark E. M.D.; Winograd, Jonathan M. M.D.; Redmond, Robert W. Ph.D.

Plastic and Reconstructive Surgery: October 2015 - Volume 136 - Issue 4 - p 739–750
doi: 10.1097/PRS.0000000000001617
Experimental: Original Articles
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Background: Nerve repair using photochemically bonded human amnion nerve wraps can result in superior outcomes in comparison with standard suture. When applied to nerve grafts, efficacy has been limited by proteolytic degradation of bonded amnion during extended periods of recovery. Chemical cross-linking of amnion before bonding may improve wrap durability and efficacy.

Methods: Three nerve wraps (amnion, cross-linked amnion, and cross-linked swine intestinal submucosa) and three fixation methods (suture, fibrin glue, and photochemical bonding) were investigated. One hundred ten Lewis rats had 15-mm left sciatic nerve gaps repaired with isografts. Nine groups (n = 10) had isografts secured by one of the aforementioned wrap/fixation combinations. Positive and negative control groups (n = 10) were repaired with graft and suture and no repair, respectively. Outcomes were assessed using sciatic function index, muscle mass retention, and histomorphometry. Statistical analysis was performed using analysis of variance and the post hoc Bonferroni test (p < 0.05).

Results: Cross-linking improved amnion durability. Photochemically bonded cross-linked amnion recovered the greatest sciatic function index, although this was not significant in comparison with graft and suture. Photochemically bonded cross-linked amnion recovered significantly greater muscle mass (67.3 ± 4.4 percent versus 60.0 ± 5.2 percent; p = 0.02), fiber diameter, axon diameter, and myelin thickness (6.87 ± 2.23 μm versus 5.47 ± 1.70 μm; 4.51 ± 1.83 μm versus 3.50 ± 1.44 μm; and 2.35 ± 0.64 μm versus 1.96 ± 0.47 μm, respectively) in comparison with graft and suture.

Conclusion: Light-activated sealing of cross-linked human amnion results in superior outcomes when compared with conventional suture.

Boston, Mass.; and Bethesda, Md.

From the Division of Plastic Surgery and the Wellman Center for Photomedicine, Massachusetts General Hospital; and the Plastic Surgery Service and the Department of Orthopedics, Walter Reed National Military Medical Center.

Received for publication August 19, 2014; accepted March 26, 2015.

The last two authors are joint senior authors on this article.

Presented at the 12th Quadrennial Congress of the European Society for Plastic, Reconstructive and Aesthetic Surgeons, in Edinburgh, Scotland, July 6 through 11, 2014; the 55th Annual Meeting of the New England Society of Plastic and Reconstructive Surgeons, in Sebasco Harbor, Maine, June 6 through 8, 2014; and the 2014 American Society for Peripheral Nerve Meeting, in Kauai, Hawaii, January 8 through 14, 2014.

Disclosure: The authors have no financial interest to declare in relation to the content of this article.

Neil G. Fairbairn, M.D., Department of Plastic Surgery, Massachusetts General Hospital, 55 Fruit Street, Boston, Mass. 02114, ngf174@hotmail.com

©2015American Society of Plastic Surgeons