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Observations on the Survival and Neovascularization of Fat Grafts Interchanged between C57BL/6-gfp and C57BL/6 Mice

Zhao, Jianhui M.D.; Yi, Chenggang M.D., Ph.D.; Li, Long M.D.; Zheng, Yan M.D., Ph.D.; Wu, Kangkang M.D.; Liang, Lihua M.D.; Xia, Wei M.D., Ph.D.; Guo, Shuzhong M.D., Ph.D.

Plastic and Reconstructive Surgery: September 2012 - Volume 130 - Issue 3 - p 398e–406e
doi: 10.1097/PRS.0b013e31825dbfd3
Experimental: Original Articles

Background: Autologous fat transplantation has become a prevalent option for soft-tissue augmentation throughout the body. However, there is still much controversy over whether the fat grafts have survived or have been replaced in the recipient sites and over how the vessels grow.

Methods: After C57BL/6-gfp mice and C57BL/6 mice were paired randomly, the inguinal fat was excised and cut into pieces with scissors, and the adipose granules, approximately 0.2 ml (0.195 g), were transplanted subcutaneously with syringes to the dorsa of the paired mice. Samples were obtained at different time intervals: 3 days, 7 days, 2 weeks, 4 weeks, 2 months, 3 months, and 4 months after transplantation. Each fat sample was weighed to evaluate the graft volume. Histology, origin, and densities of neovascularization were examined by immunohistochemical staining.

Results: At 4 months, there was no significant difference in either graft survival or histologic evaluation. Histologic evaluation manifested the normal physiologic process of inflammation, neovascularization, remodeling, and maturity at different time intervals. At the endpoint, the immunohistochemical staining of CD34 showed that the difference in capillary density of the fat graft—31.3 ± 3.9 capillaries/mm2 on the dorsa of the C57BL/6-gfp mice and 29.6 ± 3.2 capillaries/mm2 on the dorsa of the C57BL/6 mice—was not statistically significant. The α-smooth muscle actin staining indicated that there were neovascularized vessels in both C57BL/6-gfp and C57BL/6 fat grafts.

Conclusions: Fat grafts can survive and neovascularized vessels can grow from the recipient sites. Fat transplantation is feasible and will be applied more widely if fat graft survival is improved.

Xi'an, Shaanxi, People's Republic of China

From the Institute of Plastic Surgery, Xijing Hospital, and the Departments of Oral and Maxillofacial Surgery and Implantation, School of Stomatology, Fourth Military Medical University.

Received for publication November 13, 2011; accepted March 22, 2012.

The first two authors contributed equally to this research.

Disclosure: The authors have no conflicts of interest to disclose.

Shuzhong Guo, M.D., Ph.D.; Institute of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, 15 Changlexilu, Xi'an City, Shaanxi 710032, People's Republic of China,

©2012American Society of Plastic Surgeons