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Long-Term Persistence of Tissue-Engineered Adipose Flaps in a Murine Model to 1 Year: An Update

Findlay, Michael W. M.B.B.S., Ph.D.; Messina, Aurora Ph.D.; Thompson, Erik W. Ph.D.; Morrison, Wayne A. M.D.

Plastic and Reconstructive Surgery: October 2009 - Volume 124 - Issue 4 - p 1077-1084
doi: 10.1097/PRS.0b013e3181b59ff6
Experimental: Original Articles

Background: Tissue engineering of patient-specific adipose tissue has the potential to revolutionize reconstructive surgery. Numerous models have been described for the production of adipose tissue with success in the short term, but little has been reported on the stability of this tissue-engineered fat beyond 4 months.

Methods: A murine model of de novo adipogenesis producing a potentially transplantable adipose tissue flap within 4 to 6 weeks was developed in the authors’ laboratory. In this study, the authors assess the ability of three-chamber (44-μl volume) configurations shown to be adipogenic in previous short-term studies (autograft, n = 8; open, n = 6; fat flap, n = 11) to maintain their tissue volume for up to 12 months in vivo, to determine the most adipogenic configuration in the long term.

Results: Those chambers having the most contact with existing vascularized adipose tissue (open and fat flap groups) showed increased mean adipose tissue percentage (77 ± 5.6 percent and 81 ± 6.9 percent, respectively; p < 0.0007) and volume (12 ± 6.8 μl and 30 ± 14 μl, respectively; p < 0.025) when compared with short-term controls and greater adipose tissue volume than the autograft (sealed) chamber group (4.9 ± 5.8 μl; p = 0.0001) at 1 year. Inclusion of a vascularized fat flap within the chamber produced the best results, with new fat completely filling the chamber by 1 year.

Conclusions: These findings demonstrate that fat produced by tissue engineering is capable of maintaining its volume when the appropriate microenvironment is provided. This has important implications for the application of tissue-engineering techniques in humans.

Melbourne, Australia

From the Bernard O’Brien Institute of Microsurgery and the Department of Surgery, St. Vincent’s Hospital Melbourne, University of Melbourne.

Received for publication December 9, 2008; accepted April 21, 2009.

Disclosure: None of the authors has any commercial associations or financial disclosures that might pose or create a conflict of interest with information presented in this article.

Michael W. Findlay, M.B.B.S., Ph.D.; Bernard O’Brien Institute of Microsurgery; St. Vincent’s Hospital Melbourne; Melbourne, Australia;

©2009American Society of Plastic Surgeons