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Effect of Cultured Endothelial Cells on Angiogenesis in Vivo

Soejima, Kazutaka M.D.; Negishi, Naoki PhD.; Nozaki, Motohiro M.D.; Sasaki, Kenji M.D.

Plastic and Reconstructive Surgery: May 1998 - Volume 101 - Issue 6 - p 1552–1560
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The purpose of this study is to evaluate the effect of cultured endothelial cells on angiogenesis in vivo. Endothelial cells obtained from thoracic aorta of male Wistar rats were cultured in thermoresponsive dishes, which are tissue culture polystyrene dishes bound with thermoresponsive poly (N-isopropylacrylamide). Using the thermoresponsive dishes, a confluent layer of endothelial cells can be detached as an intact sheet by low temperature treatment. The obtained sheets of cultured endothelial cells were grafted to 3 X 3 cm full-thickness skin defects that had been made on the backs of rats in combination with either free skin grafts or artificial dermis grafts. Histologic examinations were performed. The findings showed that, with each of the grafting procedures, the number of vessels in a unit area (1.0 X 10-4 mm2) was significantly larger in the group with transplantation of cultured endothelial cells. This result suggests that the cultured vascular endothelial cells exert an angiogenic effect at the graft site. (Plast. Reconstr. Surg. 101: 1552, 1998.)

Tokyo, Japan

Kazutaka Soejima, M.D. 7820 Seawall Blvd. #233 Galveston, Texas 77551

From the Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical College. Received for publication November 10, 1996; revised June 2, 1997.

©1998American Society of Plastic Surgeons