Pediatric COVID-19 and Appendicitis: A Gut Reaction to SARS-CoV-2? : The Pediatric Infectious Disease Journal

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Pediatric COVID-19 and Appendicitis: A Gut Reaction to SARS-CoV-2?

Malhotra, Amisha MD*; Sturgill, Marc PharmD; Whitley-Williams, Patricia MD*; Lee, Yi-Horng MD; Esochaghi, Chika MD§; Rajasekhar, Hariprem MD; Olson, Birk BA; Gaur, Sunanda MD*

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The Pediatric Infectious Disease Journal 40(2):p e49-e55, February 2021. | DOI: 10.1097/INF.0000000000002998
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We describe the temporal pattern of COVID-19 admissions to a tertiary care children’s hospital in central New Jersey during the SARS-CoV-2 surge, covering the time period from March 29 to July 26, 2020.


Medical charts were reviewed for the date of admission, past medical history, and demographic variables, presenting signs and symptoms, admitting laboratory values, diagnostic imaging, diagnosis, treatment modalities, and outcomes including length of stay and disease severity.


Patients with symptomatic SARS-CoV-2 infection tended to present with pneumonia early during the study period, which coincided with the early surge in New Jersey cases. Approximately 2 weeks after the peak in reported SARS-CoV-2 cases in New Jersey, we began to see fewer pneumonia cases and an increase in admissions for Multi-Inflammatory Syndrome in Children and cases of acute appendicitis in association with a diagnosis of SARS-CoV-2 infection.


We present a novel association of acute appendicitis in children infected with SARS-CoV-2 and postulate that it may represent a postinfectious hyperinflammatory complication of SARS-CoV-2 infection occurring 2 weeks after the early manifestation of acute pneumonia disease in children.

On March 11, 2020, the World Health Organization declared the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak as a global pandemic.1,2 As of June 28, 2020, the US Centers for Disease Control and Prevention (CDC) reported 2504,175 confirmed SAR-CoV-2 cases and 125,484 deaths in the United States.3 Fortunately, children represent only a fraction of reported US cases, accounting for only 5.49 percent of confirmed SARS-CoV-2 cases in the June 28, 2020, CDC report.3

Children appear to be as likely as adults to be infected with SARS-CoV-2 but are more likely to remain asymptomatic or develop only mild upper respiratory tract symptoms—they may therefore be less likely to get tested and receive a confirmed diagnosis.4–6 A CDC report revealed that children were less likely than adults to experience fever, cough, or dyspnea (73% versus 95%) or to require hospitalization (5.7% versus 10%).4 Two epidemiologic studies of confirmed SARS-CoV-2 cases from China showed similar results.5,6 Dong et al described 728 children with PCR-confirmed SARS-CoV-2 of which 12.9% were asymptomatic and 43.1% had only mild upper respiratory tract (URI) symptoms.5 In a separate study of 171 children with confirmed SARS-CoV-2 infection 15.8% were asymptomatic, 19.3% had signs and symptoms consistent with URI, and 64.9% had CT-confirmed pneumonia.6

As of October 10, 2020, 61,364 patients had been hospitalized in the United States with confirmed SARS-CoV-2 infection, with children accounting for only 1.6 percent of hospitalizations.7 According to the CDC, children of all ages were less likely than adults to require hospitalization (estimated hospitalization rates of 5.7%–20% and 10%–33%, respectively) or intensive care unit (ICU) admission (0.58%–2.0% and 1.4%–4.5%, respectively), but hospitalization rates were higher in children less than 1 year of age.4 In their pediatric cohort of 728 PCR-confirmed SARS-CoV-2 cases, Dong et al reported a 5.2 percent incidence of severe respiratory infections with clinical progression to dyspnea and a 0.6 percent incidence of critical infections with clinical deterioration to acute respiratory distress syndrome (ARDS) and signs of other organ dysfunction.5

Although earlier reports stated that children younger than 5 years old were at greater risk of severe SARS-CoV-2 disease; in late April/early May 2020, the European Union and United States reported clusters of older children and adolescents presenting with shock, fever, and hyperinflammation associated with SARS-CoV-2 infection. In May 2020, the Centers for Disease Control and Prevention (CDC) issued a national health advisory to report on cases meeting the criteria for Multisystem Inflammatory Syndrome in Children (MIS-C). This subset of children develops a dysregulated immune response with host tissue damage and hyperinflammation, resembling Kawasaki’s disease (KD), toxic shock syndrome, or macrophage activating syndrome, with the median age of onset being 8.3 years.8–11 In cases with MIS-C, the majority of children were hospitalized (80%–88%) with most requiring intensive care management (80%) for multiorgan dysfunction.11,12 Gastrointestinal and cardiovascular organ systems were the 2 of the most commonly affected (92% and 80%, respectively).11

Gastrointestinal (GI) symptoms, such as diarrhea and abdominal pain, are a known feature of SARS-CoV-2 infection and have been reported in adults and children with mild to severe SARS-CoV-2 infection.13,14 Other SARS-CoV-2 associated GI manifestations that have been reported are ileus and mesenteric adenopathy in adults and children with terminal ileitis presenting as atypical appendicitis.13,14 Individual case reports of pseudoappendicitis and acute surgical abdomen have been reported in the literature in pediatric and adult SARS-CoV-2-positive patients.14–17

New York and New Jersey (NJ) were early centers of SARS-CoV-2 infection and spread.4 The first known NJ case was reported on March 4, 2020, followed by an exponential increase in reported cases and hospitalizations.18 We report the clinical presentation and management of 48 children admitted to the Bristol Myers Squibb Children’s Hospital (BMSCH), a tertiary care facility affiliated with Robert Wood Johnson (RWJ)—Barnabas Health in New Brunswick, NJ, with confirmed SARS-CoV-2 infection between March 29 and July 26, 2020. We compare the characteristics of cases seen during the early period of the pandemic surge to those seen during the later period, focusing on children in the later period who presented with acute appendicitis.


Study Methods

A retrospective analysis of pediatric patients with confirmed SARS-CoV-2 infection admitted between March 29, 2020, to July 26, 2020, was performed. During the pandemic, the age for BMSCH admission was increased to 25 years. Hospitalized patients were tested for SARS-CoV-2 based on suspicion of disease. Universal SARS-CoV-2 PCR screening of hospitalized patients via nasopharyngeal specimens was initiated on April 12, 2020, utilizing the Xpert Xpress SARS-CoV-2 (Cepheid, Sunnyvale, CA), Simplexa COVID-19 Direct Kit (DiaSorin Molecular LLC, Cypress, CA), or Cobas 6800 SARS-CoV-2 test (Roche Diagnostics, Indianapolis, IN) based on availability. The SARS-CoV-2 IgG assay (Abbott Diagnostics, Lake Forest, IL) became available on April 20, 2020.

Data Collection

Inclusion criteria included all hospitalized patients 25 years old or less with positive SARS-CoV-2 diagnostic testing by PCR and SARS-CoV-2 antibody testing. IRB approval was received, and patient information was deidentified. The electronic medical record was used to obtain pertinent information.

Demographic data included age, gender, race, and ethnicity, underlying comorbidities, history of SARS-CoV-2 exposure, and admission weight. Weight percentiles were determined per CDC growth charts and categorized as normal (5th to less than 85th percentile), overweight (85th to less than 95th percentile), or obese (greater than 95th percentile).19

Presenting signs and symptoms included number of days of fever before admission (PTA) and respiratory (cough, shortness of breath (SOB), rhinitis), gastrointestinal (GI; nausea, vomiting, diarrhea, abdominal pain), dermatologic (rash), or other (conjunctivitis, cervical lymphadenopathy, myalgias, dizziness, headache) signs, and symptoms. Admitting laboratory values included serum white blood cell (WBC) count, absolute lymphocyte count (ALC), absolute neutrophil count (ANC), platelet (PLT) count, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), D-dimer, fibrinogen, ferritin, prothrombin time (PT), partial thromboplastin time (PTT), and interleukin-6 (IL-6).

Clinical data included the days of fever following admission, radiologic imaging findings, the need for and duration of supplemental oxygen (O2) use, need for pediatric intensive care unit (PICU) admission, hospital length of stay (LOS), and medication regimens.

We also collected demographic and clinical data for all appendicitis cases by ICD-10 code admitted during the same timeframe, which included demographic data, GI symptoms, presence of fevers, and length of stay.

Disease Severity Classification

Disease severity was classified into 3 groups: (1) Mild—did not require supplemental oxygen, inotropes, or PICU admission; (2) Moderate—required supplemental oxygen via nasal cannula or face mask, and may have required PICU admission, but did not need intubation or inotropic support; (3) Severe—required mechanical ventilation or inotropic support in the PICU.

Statistical Methods

Normally distributed continuous demographic characteristics was presented as mean ± SD, and nonnormally distributed variables as median and interquartile range (IQR). Categorical variables were presented as frequency (percentage). Spearman Rank Order Correlation tests were used to investigate the potential relationships between demographic variables and presenting symptoms, disease severity, and hospital length of stay. A One Way Analysis of Variance (ANOVA; for normally distributed variables) or Kruskal-Wallis One Way ANOVA on Ranks (for nonnormally distributed variables) was used to compare SARS-CoV-2 pneumonia, MIS-C, and appendicitis patients with respect to continuous admitting and outcomes variables. A χ2 test was used to compare categorical admitting and outcomes data, with adjusted residuals used for post hoc testing in the case of a significant P value. A P value less than 0.05 was considered significant for all analyses.


Forty-eight patients with confirmed SARS-CoV-2 infection were admitted between March 29 and July 26, 2020 (Figure 1). On review, we classified patients into 5 separate diagnostic categories: (1) Pneumonia—respiratory signs and symptoms and chest radiograph (CXR) findings consistent with pneumonia; (2) MIS-C—met the CDC criteria for MIS-C12; also classified as having KD if they met American Heart Association guidelines for classic or incomplete KD20; (3) Appendicitis—signs, symptoms and radiographic imaging compatible with appendicitis; (4) Unconventional—signs and symptoms clinically compatible with SARS-CoV-2 but did not fit any of the other categories; (5) Asymptomatic—admitted for an unrelated diagnosis and SARS-CoV-2 positive on routine screening.

Timeline of SARS-CoV-2-positive admissions at BMSCH by diagnosis.

Seven patients were SARS-CoV-2 asymptomatic and were excluded from further analysis. Of the remaining 41 patients, 16 were diagnosed with SARS-CoV-2 pneumonia, 10 with appendicitis, 10 with MIS-C, and 5 with unconventional symptoms. As Figure 1 shows, SARS-CoV-2 pneumonia was more frequent during the first 4–6 weeks (11 of 16 cases), while all 10 appendicitis cases and 9 of 10 MIS-C cases were seen more frequently during the final 12 weeks.

Demographic characteristics of all patients are given in Table 1. The median (IQR) age of all 41 patients was 9 (0.2, 17) years. The median (IQR) age was significantly greater in patients with pneumonia or appendicitis [15 (4.25, 18.5) and 12 (6, 13.5) years, respectively] than in those with unconventional symptoms [0.275 (0.183, 1.575) years, P = 0.003]. Overall, most patients were male (56.1%) and Hispanic or Latino (51.2%). When comparing the 4 cohorts by ethnicity, there were fewer non-Hispanic children with appendicitis than expected (9 Hispanic versus only 1 non-Hispanic patient; P = 0.021). Although no other racial or ethnic differences were significant, non-Hispanic Black children predominated in the MIS-C group (6 non-Hispanic Black versus 3 Hispanic and 1 other). Fourteen patients (34.1%) had underlying comorbidities, and 19 (46.3%) had exposure to a confirmed or suspected SARS-CoV-2 case before admission (PTA). Patients diagnosed with appendicitis or MIS-C were significantly less likely than expected to have comorbidities (only 1 child in the appendicitis and MIS-C cohort; P = 0.012). There was a nonsignificant trend toward a higher median weight percentile in MIS-C and pneumonia patients. There were no other differences in demographic variables. Patients with SARS-CoV-2 unconventional infection were excluded from further analysis.

TABLE 1. - Demographic Characteristics
Appendicitis (n = 10) MIS-C (n = 10) Pneumonia (n = 16) Unconventional (n = 5) P
Age (yrs) [median (IQR)] 12 (6–13.5) 9 (5.75–12.5) 15 (4.25–18.5) 0.3 (0.205–1.5)†‡ 0.003*
Gender [no. (%)] F, 4 (40.0) F, 3 (30.0) F, 9 (56.25) F, 2 (40.0) NS§
M, 6 (60.0) M, 7 (70.0) M, 7 (43.75) M, 3 (60.0)
Ethnicity [no. (%)] W, 1 (10.0) W, 0 W, 5 (31.25) W, 1 (20.0)
B, 0 B, 6 (60.0) B, 3 (18.75) B, 0
H, 9 (90.0) H, 3 (30.0) H, 7 (43.75) H, 2 (40.0)
O, 0 O, 1 (10.0) O, 1 (6.25) O, 2 (40.0)
Ethnicity [no. (%)] H, 9 (90.0) H, 3 (30.0) H, 7 (43.75) H, 2 (40.0) 0.021§
non-H, 1 (10.0) non-H, 7 (70.0) non-H, 9 (56.25) non-H, 3 (60.0)
Median (IQR) weight percentile (%) 75 90 87 79.5 NS*
(47.5–91.25) (83–99) (38.75–99) (16.5–99)
Comorbidities [no. (%)] Yes, 1 (10.0) Yes, 1 (10.0) Yes, 10 (62.5) Yes, 2 (50.0) 0.012§
No, 9 (90.0) No, 9 (90.0) No, 6 (37.5) No, 2 (50.0)
Exposure to confirmed case [no. (%)] Yes, 3 (30.0) Yes, 4 (40.0) Yes, 9 (56.25) Yes, 3 (40.0) NS§
No, 7 (70.0) No, 6 (60.0) No, 7 (43.75) No, 2 (60.0)
Asymptomatic COVID patients were excluded from this analysis.
*Kruskal-Wallis one way analysis of variance on ranks for comparison of SARS-CoV-2 pneumonia, MIS-C, and appendicitis patients.
†‡P < 0.05 by Dunn’s all pairwise multiple comparison procedures method.
§χ2 test for comparison of SARS-CoV-2 pneumonia, MIS-C, and appendicitis patients.
P < 0.05 adjusted residual post hoc method.
F indicates female; M, male; W, non-Hispanic White; B, non-Hispanic Black; H, Hispanic or Latino; O, other; PTA, before hospital admission; NS, not significant; IQR, interquartile range; MIS-C, multiinflammatory syndrome in children.

Clinical characteristics and outcomes are given in Table 2. Overall, among the 3 cohorts, there was a significant correlation between the days of fever PTA (P ≤ 0.001) disease severity (P = 0.008), and hospital LOS (P = 0.049). Patients with appendicitis had significantly fewer days of fever PTA [0 (0, 1.25)] than did patients with MIS-C or pneumonia [5 (4, 6) and 5 (1.25, 7.75) days, respectively; P<0.001]. Appendicitis patients presented predominantly with nausea, vomiting, and abdominal pain. Both MIS-C and pneumonia patients had longer febrile days both before admission and while in hospital. MIS-C patients were also noted to present with gastrointestinal features, similar to appendicitis patients, but reported a higher frequency of diarrheal symptoms at time of presentation. Patients with appendicitis had a significantly lower median (IQR) disease severity ranking than either pneumonia or MIS-C patients and were significantly less likely than MIS-C patients to require ICU admission. Appendicitis patients also had a significantly shorter hospital LOS than did patients with pneumonia [3 (1, 8) versus 7.5 (6, 16.25) days, respectively; P = 0.049]. One death occurred in a patient with SARS-CoV-2 pneumonia, but this patient was admitted with terminal acute myelocytic leukemia.

TABLE 2. - Comparison of Clinical Characteristics and Outcomes by Diagnosis
Appendicitis (n = 10) MIS-C (n = 10) Pneumonia (n = 16) P
Days fever PTA [median (IQR)] 0 (0, 1.25)†‡ 5 (4, 6) 5 (1.25, 7.75) <0.001*
Days fever in hospital [median (IQR)] 0.5 (0, 2.5) 1.5 (0, 2.75) 1 (1, 3) NS*
Disease severity [median (IQR)] 1 (1, 1)†‡ 2.5 (1, 3) 2 (1.25, 2) 0.008*
Hospital LOS [median (IQR)] 3 (1, 8) 9 (4.5, 12) 7.5 (6, 16.25) 0.049*
*Kruskal-Wallis one way analysis of variance on ranks.
†‡P < 0.05 by Dunn’s all pairwise multiple comparison procedures method.
PTA indicates before hospital admission; LOS, length of stay; NS, not significant.

Admitting laboratory values are given in Table 3. Appendicitis and MIS-C patients had significantly higher WBC and ANC values than pneumonia patients. CRP values were significantly higher in MIS-C patients than in pneumonia patients. Appendicitis patients had significantly lower AST and LDH values than did patients with MIS-C or pneumonia and significantly lower ALT values than patients with MIS-C. There was a trend toward a higher ALC and lower D-dimer value in patients with appendicitis, but it should be noted that age-adjusted ALC values were low in most patients (56.25%, 25%, 60%, respectively of patients with SARS-CoV-2 pneumonia, appendicitis, and MIS-C) and D-dimer values elevated in most patients (56.25%, 25%, and 60%, respectively, of patients with SARS-CoV-2 pneumonia, appendicitis, and MIS-C).

TABLE 3. - Comparison of Median (IQR) Admitting Inflammatory Markers by Clinical Diagnosis
Appendicitis (n = 10) MIS-C (n = 10) Pneumonia (n = 16) P
WBC (103 cells/mm3) 12.75 (10.075, 16.5) 12.6 (8.9, 20.45) 5.15 (3.475, 11.125) 0.002*
ALC (103 cells/mm3) 1.15 (0.785, 2.17) 1.04 (0.54, 1.652) 1.07 (0.86, 1.6) NS*
ANC (103 cells/mm3) 11.19 (7.415, 15.16) 10.78 (4.99, 18.65) 3.5 (2.15, 6.3) 0.002*
ESR (mm/h) 27 (18.5, 70) 43 (31.25, 78.75) 56 (20, 84.75) NS*
CRP (mg/dL) 9.62 (4.278, 20.255) 27.395 (10.668, 30.502) 6.17 (2.63, 8.565) 0.001*
AST (U/L) 20 (14.25, 25) 57.5 (35.5, 68.75) 49.5 (27.75, 86) 0.004*
ALT (U/L) 15 (9, 19) 45 (20.25, 71) 34 (12.75, 63.25) 0.016*
LDH (U/L) 184 (135, 210.5) 297 (259.75, 457.25) 288.5 (201.75, 605.5) 0.020*
D-dimer (ng FEU/mL) 930 (318.75, 10,162.75) 2372 (1447, 4175) 1082.5 (641.75, 1589) NS*
Fibrinogen (mg/dL) 571.5 (390.5, 733) 690.5 (581, 848) 714 (489, 795) NS*
Ferritin (ng/mL) 107 (78, 181) 664.5 (327.5, 1293.25) 378.5 (160, 1133.75) 0.015*
*Kruskal-Wallis one way analysis of variance on ranks for comparison of SARS-CoV-2 pneumonia, MIS-C, and appendicitis patients.
P < 0.05 by Dunn’s all pairwise multiple comparison procedures method.
NS indicates not significant; IQR, interquartile range; ALC, absolute lymphocyte count; ANC, absolute neutrophil count; ESR, erythrocyte sedimentation rate; CRP, C-reactive protein; AST, aspartate aminotransferase; ALT, alanine aminotransferase; LDH, lactate dehydrogenase.

Table 4 compares the demographic characteristics of our 10 SARS-CoV-2-positive appendicitis patients to 61 patients admitted to the BMSCH for acute appendicitis during the same time period who tested negative for SARS-CoV-2. When comparing patients with appendiceal rupture, there were significantly fewer non-Hispanic or Latino patients than expected in the SARS-CoV-2 negative group (P = 0.047).

TABLE 4. - Comparison of SARS-CoV-2 Positive and Negative Appendicitis Patients by Demographics, Rupture, and Days of GI Symptoms Before Admission
Appendiceal Rupture No Rupture
SARS-CoV-2 Positive (n = 5) SARS-CoV-2 Negative (n = 22) P SARS-Cov-2 Positive (n = 5) SARS-CoV-2 Negative (n = 39) P
Age (yrs) Mean ± SD 11.4 ± 5.98 11.0 ± 3.67 NS* 9.75 ± 4.425 12.1 ± 4.90 NS*
Gender no. (%) M, 2 (40.0) M, 15 (68.2) NS M, 4 (80.0) M, 20 (51.3) NS
F, 3 (60.0) F, 7 (31.8) F, 1 (20.0) F, 19 (48.7)
Ethnicity no. (%) H, 5 (100) H, 10 (45.5) 0.047 H, 4 (80.0) H, 17 (43.6) NS
Non-H, 0 Non-H, 12 (54.5) Non-H, 1 (20.0) Non-H, 22 (56.4)
Days of GI symptoms PTA [median (IQR)] 4 (2.5, 5) 2 (1, 3) NS§ 1 (0.75, 2.5) 1 (1, 2) NS§
*Student’s t test.
†Fisher’s exact test.
P<0.05 adjusted residual post hoc method.
§Mann-Whitney rank-sum test.
GI indicates gastrointestinal; PTA, before admission; M, Male; F, Female; H, Hispanic; non-H, non-Hispanic; SD, standard deviation; IQR, interquartile range.

Clinical Course

SARS-CoV-2 Pneumonia

Fifteen of the 16 pneumonia patients were SARS-CoV-2 PCR-positive; 1 patient was SARS-CoV-2 antibody positive. Multilobar disease was the most common CXR finding, noted in 10 patients, with ground glass opacities in only 2 patients. Presenting clinical characteristics noted most often included fever, cough, and shortness of breath.


Ten children met the CDC criteria for MIS-C. Three were SARS-CoV-2 PCR-positive, and 6 were PCR-negative and SARS-CoV-2 antibody positive; 1 patient was both SARS-CoV-2 PCR and antibody positive.

Five of the 10 MIS-C patients met the criteria for KD, and the most prominent symptoms noted with fever were maculopapular rash and conjunctivitis. Eight of the 10 MIS-C patients had echocardiographic abnormalities suggestive of myocarditis, and 2 of these patients had abnormal coronaries.

Unconventional SARS-CoV-2

The 5 unconventional COVID-19 cases that were admitted were all children 2 years of age and under (ages 0.16, 2 years) who presented with a variety of symptoms. Patient 1 was a 7-week-old white male admitted with fever and unilateral conjunctivitis with a normal CXR. He had 48 hours of fever and was discharged home on hospital day 3 with resolution of symptoms. Patient 2 was a 2-year-old Hispanic female with chronic medical conditions (holoprosencephaly, chronic lung disease, seizure disorder, failure to thrive, and developmental delay) admitted with fever for 1 day and shortness of breath, requiring oxygen by nasal cannula. CXR showed peribronchial thickening, consistent with her chronic lung disease, and no infiltrates. Patient 3 was a 3-month-old ex-34-week gestation male admitted with vomiting and shortness of breath. CXR showed peribronchial thickening with no infiltrates. Patient 4 was a 1-year-old female admitted with vomiting and abdominal pain and no fever. She had a normal CXR, and abdominal CT scan showed small bowel obstruction with no appendicitis. She had an exploratory laparotomy with excision of an adhesion in the distal ileum and was discharged on the sixth hospital day. Patient 5 was a 4-month-old Hispanic male admitted with fever for 1 day, vomiting with shortness of breath and cough. His CXR was normal, and he was discharged within 48 hours of rehydration. Three of the 5 children had a household COVID-19 contact. All 5 children were SARS-CoV-2 PCR positive.


Five of the 10 children with a radiologic diagnosis of appendicitis had a ruptured appendix, and only 1 of 8 had radiologic evidence of mesenteric adenopathy. Eight of the 10 patients underwent an appendectomy, and the other 2 were medically managed.

The major presenting clinical findings were fever, nausea, vomiting, and abdominal pain. CXR was performed in 6 children, and 3 had abnormal findings (unilateral or bilateral basilar opacity); however, none had respiratory symptoms. Three of the 7 appendicitis patients were overweight or obese.

When comparing SARS-CoV-2-positive and negative appendicitis patients with appendiceal rupture to those without, there was a trend in both groups toward a longer duration of GI symptoms before admission in children with appendiceal rupture (Table 5). The patients with nonruptured appendicitis in both groups presented earlier to the hospital with shorter days of GI symptoms as compared with the cases with ruptured appendicitis, and although not significant, it was noted that those with SARS-CoV-2 infection had a longer duration of symptoms before admission than those that were negative. None of the SARS-CoV-2 positive appendicitis patients received remdesivir, steroids, or biologics during their hospital stay.

TABLE 5. - Comparison of Rupture and Nonrupture Appendicitis in SARS-CoV-2 Positive Patients by Clinical Course
Ruptured Appendix (n = 5) Nonruptured Appendix (n = 5) P
Days of GI symptoms PTA [median (IQR)] 4 (2.5, 5) 1 (0.75, 2.5) 0.032*
Days fever PTA [median (IQR)] 0 (0, 2) 0 (0, 1) NS*
Days fever in hospital [median (IQR)] 2 (0.5, 4.5) 0 (0, 0.5) NS*
Disease severity [median (IQR)] 1 (1, 1.5) 1 (1, 1) NS*
Hospital LOS [median (IQR)] 8 (4.25, 12.5) 1 (1, 2) 0.016*
*Mann-Whitney Rank Sum Test.
†Fisher’s Exact test.
PTA indicates before hospital admission; GI, gastrointestinal; LOS, length of stay; NS, not significant


We report our experience with pediatric cases of SARS-CoV-2 infection in children hospitalized at BMSCH. In total, we admitted 48 patients with a diagnosis of SARS-CoV-2 infection in the first 4 months of the peak epidemic in New Jersey. Pneumonia and MIS-C are well-known associations with pediatric SARS-CoV-2 disease; however, we also report a cluster of 10 SARS-CoV-2-positive children admitted with acute appendicitis during the New Jersey pandemic between March 29 and July 26, 2020.5,6,11 Appendicitis accounted for 20.8% of the children admitted during this time period who tested positive for SARS-CoV-2. The pattern of admissions (Figure 1) followed the overall trend of primarily seeing SARS-CoV-2 pneumonia cases early, particularly during the first 6 weeks, followed approximately 4 weeks later by admissions for MIS-C and acute appendicitis.

Pneumonia is a well-known clinical manifestation of acute SARS-CoV-2 infection, which was reflected in our 16 patients with presenting signs, symptoms, laboratory variables, chest radiographs, and clinical course that has been previously reported.5,6

The clinical presentation, course, and management of our MIS-C patients were similar to previous reports concerning this condition in children.10,11,21–24 Six of the 10 children were overweight or obese, with a seventh child at the 84th weight percentile. Most of our MIS-C patients had cardiac injury with some level of carditis (8 of 10) and presented with ventricular dysfunction and cardiogenic shock, as has been noted in previous studies.10,11,21–24 Ethnic minorities accounted for the majority of patients.

MIS-C may represent a postinfectious hyperinflammatory complication of SARS-CoV-2 since it presents later in the timeline of the epidemic, and MIS-C patients are often PCR negative, but antibody-positive suggesting a late manifestation. Of the 99 children with MIS-C reported by Dufort et al, only 19% were PCR-positive and antibody-negative, while 33% were positive by both assays and 47% antibody-positive and PCR-negative, an observation that supports the concept of MIS-C being a postinfectious complication.10 Serologic testing for antibodies to SARS-CoV-2 was not available at our institution until April 20, 2020. Seven of the 10 appendicitis cases were PCR positive (the remaining 2 were antibody positive), while 6 of the 10 patients with MIS-C were antibody positive (the remainder 3 were PCR positive). One patient in each category was both PCR and antibody positive. Also, to note, antibody testing was not routinely done at our institution for the appendicitis and MIS-C cases if they were PCR positive, so we cannot comment on possible antibody positivity in addition to PCR positivity in these cases. It is possible that many of the PCR positive appendicitis cases may have also tested antibody positive given that they presented later on our epidemic curve.

In our cohort, all 5 young infants classified as having unconventional disease were managed on the general Pediatric floor, had mild to moderate symptoms and did not progress to severe disease as has previously been reported for this age group.4,5 Three of the 5 children presented with gastrointestinal symptoms, as has been observed in other studies.13,25 Interestingly, intussception has been described in infants with SARS-CoV-2 infection and we postulate that SARS-CoV-2 infection may have been an inciting factor for small bowel obstruction observed in our patient.

We admitted 10 SARS-CoV-2 positive patients and 61 SARS-CoV-2 negative patients with acute appendicitis between March 29 and July 26, 2020. Ninety percent of these children had no underlying comorbidities, and 50% were overweight or obese. Nine of the 10 SARS-CoV-2 positive patients were Hispanic or Latino. Previous studies have clearly established that Hispanic or Latino and non-Hispanic Black communities are being disproportionately impacted by the SARS-CoV-2 pandemic.10,11,26,27 Due to a variety of socioeconomic barriers including poor access to care, these patients are more likely to be hospitalized with SARS-CoV-2 and more likely to experience a severe disease course.

Although not statistically significant, the racial and ethnic distribution of the children in the 2 appendicitis cohorts was different. There was no difference in the race/ethnic makeup in the SARS-CoV-2 negative group, whereas 9 of the 10 patients in the SARS-CoV-2 positive group were Hispanic. This reiterates the fact that minority groups are disproportionately being affected with SARS-CoV-2.

Racial and ethnic minorities are also at a greater risk for appendiceal rupture in the United States, due to poor access to healthcare.28,29 Ponsky et al reported an increased rupture rate in minority children when compared with White children [adjusted OR (95% CI) ratio of appendiceal rupture in Black children of 1.13 (1.01–1.3) and Asian children of 1.66 (1.24–2.23)]. Smink et al found a similar relationship [OR (95% CI) of appendiceal rupture was 1.23 (1.1–1.39) for Black and 1.20 (1.1–1.3) for Hispanic children, compared with White children].29 In contrast, our study did not find a significant racial difference in patients with appendiceal rupture (data not shown).

When comparing SARS-CoV-2-positive and negative appendicitis patients, some more intriguing observations emerged. In SARS-CoV-2-negative patients, the overall rupture rate was 36.1%; in contrast, 50% of SARS-CoV-2-positive children experienced appendiceal rupture. Established risk factors for appendiceal rupture in children revolve around delays in diagnosis and the risk of perforation increases dramatically if left untreated beyond 48 hours. Age is therefore an important risk factor, as infants and many preschoolers are unable to verbalize their symptoms and accurately locate the region of pain.28,30 Accordingly, infants and children less than 3 years of age are more likely to be misdiagnosed early on and have a reported perforation rate of 82–92%.30 Ponsky et al reported that children between 5 and 12 years of age were more likely than those 13–17 years of age to present with ruptured appendix (OR and 95% CI was 1.41 (1.3,1.53)].28 In our study, we did not see an age-related difference for the ruptured appendicitis patients in both groups with the median (IQR) age of 12 years in the SARS-CoV-2 positive group and 11 years in the SARS-CoV-2 negative group. The overall rupture rate among the 61 SARS-CoV-2-negative patients (36.1%) correlates well with that reported by Ponsky et al; however, a 50% rate was observed in our 10 SARS-CoV-2-positive patients. If we assume that a delay in obtaining healthcare due to fear of exposure to SARS-CoV-2 in hospitals was the reason for an increase in ruptured appendicitis cases, then the SARS-negative cohort should have been equally affected. Although our numbers are small, we postulate that an exaggerated inflammatory response may be at play among SARS-CoV-2 infected patients leading to rupture.

The cause of appendicitis is unknown, although obstruction of the lumen of the appendix secondary to an initiating factor such as appendicolith formation or mesenteric adenopathy is suspected.31 All 3 of our patients with rupture who were screened for appendicoliths were positive, compared with 2 of 5 patients without rupture. Mesenteric lymphadenopathy was noted in 1 of 3 with rupture and none of the patients without rupture.

Could acute appendicitis represent another postinfectious, hyperinflammatory complication of SARS-CoV-2, or rather does acute GI infection and inflammation trigger the development of appendicitis? ACE2 receptors are widely distributed in both smooth muscle and endothelial cells of the stomach, small intestine, and colon, as well as arterial and venous endothelial cells throughout the body.32 The exact cause of appendicitis remains poorly understood. Although family clusters and a seasonal pattern have been observed, consistent evidence of a viral trigger is lacking.33,34

Cai and colleagues reported on 5 SARS-CoV-2-positive children presenting with GI signs and symptoms, 1 with CT-confirmed acute appendicitis with rupture and localized peritonitis.25 Previous reports suggest that patients with SARS-CoV-2 infection can present with GI signs and symptoms suggestive of acute appendicitis but subsequently ruled out by abdominal CT.13,15,35 In contrast to the reports of pseudo-appendicitis, our experience shows that appendicitis can, in fact occur in SARS-CoV-2 infected pediatric patients.

In conclusion, we describe an experience with SARS-CoV-2 infection in children seen at a tertiary care hospital in central NJ who presented with different clinical manifestations during the SARS-CoV-2 surge in NJ. The temporal pattern of admissions suggests that pneumonia is likely to be seen early during an epidemic curve, followed by hyperinflammatory postinfectious manifestations such as MIS-C. To the best of our knowledge, this is the first report of a temporal association of SARS-CoV-2 infection with multiple cases of acute appendicitis in children. Although our numbers are small and more studies are needed to verify this association, clinicians should be made aware of the possibility that appendicitis can present as a postinfectious manifestation of SARS-CoV-2 infection in children.


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SARS-CoV-2; COVID-19; children; MIS-C; pneumonia; appendicitis

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