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Safety and Immunogenicity of a Quadrivalent Meningococcal Conjugate Vaccine in Healthy Meningococcal-Naïve Children 2–9 Years of Age: A Phase III, Randomized Study

Baccarini, Carmen I. MD*; Simon, Michael W. MD, PhD; Brandon, Donald MD; Christensen, Shane MD, FAAFP, CCRP§; Jordanov, Emilia MD*; Dhingra, Mandeep S. MD*

Author Information
The Pediatric Infectious Disease Journal: October 2020 - Volume 39 - Issue 10 - p 955-960
doi: 10.1097/INF.0000000000002832



Neisseria meningitidis is responsible for invasive meningococcal disease (IMD) presenting most commonly as meningitis and septicemia. Children are particularly vulnerable.1 Survivors may experience long-term sequelae including amputation, loss of hearing, brain damage, and neurologic impairments. In Europe, the United States, and Canada, the incidence of IMD cases per 100,000 population was 0.70, 0.12, and 0.30, respectively, in 2015.2N. meningitidis can be classified into 12 meningococcal serogroups, of which 6 (A, B, C, W, X, and Y) are considered to be the primary cause of IMD worldwide.3 However, the prevalence of the serogroups varies over time by age and region. In Europe, the most common cause of IMD is serogroup B; in recent years, cases caused by serogroup W and Y have increased.4 In the United States in 2017, there were approximately 350 total cases of IMD reported, with serogroups B, C, and Y the most common cause.5 In the United States, serogroups B and C are also the most common cause of IMD, with incidence rates up to 4.5 per 100,000 reported.6 In the Asia Pacific region, IMD is underrecognized, with limited data indicating that serogroups B, C, and W are dominant.7 Serogroups A and W have been the predominant cause of IMD in Africa,8 with rates of disease exceeding 200 cases per 100,000 population reported in several countries.6 In general, the incidence of IMD is highest in children under 5 years of age.8 Accurate global incidence rates are difficult to ascertain due to limitations in surveillance and underreporting, particularly in developing countries. The global case-fatality ratio of IMD has been estimated to be 10%–15% even with appropriate antibiotic treatment.9,10

Meningococcal vaccines have helped to reduce the incidence of IMD to <1 case per 100,000 population per year in developed countries with established meningococcal vaccine programs.3 In sub-Saharan Africa, the introduction of the meningococcal serogroup A vaccine has led to the virtual elimination of this serogroup as a cause of epidemic IMD in some countries in the region.11,12 Nonetheless, the disease incidence continues to vary geographically overtime, with periodic epidemics/outbreaks caused by different IMD serogroups. The temporal dynamic changes in the disease-causing serogroups highlights the need for meningococcal vaccines that provide protection against a broad range of serogroups.8

An investigational quadrivalent meningococcal polysaccharide (serogroups A, C, Y, and W) tetanus toxoid conjugate vaccine (MenACYW-TT, Sanofi Pasteur) has been developed for use in individuals ≥6 weeks of age. We compared the immunogenicity and safety profile of a single dose of MenACYW-TT with a licensed quadrivalent diphtheria protein (CRM197) conjugate meningococcal vaccine (MenACWY-CRM, Menveo®, GlaxoSmithKline Vaccines Srl; formerly Novartis Vaccines) in children 2–9 years of age in the United States, including Puerto Rico.


Study Design and Participants

This was a Phase III, modified double-blind, randomized, parallel-group, active-controlled study conducted from February 2017 to October 2017 at 36 sites across the United States including Puerto Rico ( NCT03077438).

The appropriate independent ethics committees and institutional review boards approved the study before its initiation. The conduct of this study was consistent with standards established by the Declaration of Helsinki and compliant with the International Conference on Harmonisation guidelines for Good Clinical Practice, including all local and national regulations and directives. Parents or guardians were required to provide signed informed consent before the start of the study. In addition, participants (≥7 years of age) were asked to sign an assent form (as required by local regulations).

Healthy meningococcal vaccine-naïve children 2–9 years of age were recruited. Participants were excluded if they had been involved in another clinical trial of a drug, vaccine, or medical device in the 4 weeks before the start of the present study, or had planned simultaneous participation in another trial. Those who received another vaccine in the 4 weeks preceding the study vaccination or planned to receive another vaccine before immunologic assessment at day 30 were also excluded. Other exclusion criteria included a history of Guillain-Barré syndrome and meningococcal infection confirmed clinically, serologically, or microbiologically, or a demonstrable high risk of meningococcal infection during the study. Participants with known systemic hypersensitivity to any of the vaccine’s components, or a history of a life-threatening reaction to these components, were also excluded.

Eligible participants were randomly assigned in a 1:1 ratio via an interactive web response system (stratified by the age groups of 2–5 and 6–9 years) to receive a single dose (0.5 mL) of either MenACYW-TT conjugate vaccine or MenACWY-CRM at day 0. Participants, their parents/guardians, and investigators were unaware of treatment assignments throughout the study. The vaccines were administered by an unblinded administrator who was not involved in safety data collection. Serology testing was performed by the sponsor and laboratory personnel who remained blinded to treatment assignments throughout the study.

MenACYW-TT (Sanofi Pasteur, Swiftwater, PA) was presented as a liquid solution in single-dose vials; each 0.5 mL dose contained 10 μg of each serogroup (A, C, W, and Y) and approximately 55 μg of tetanus toxoid protein carrier. MenACWY-CRM (GlaxoSmithKline Vaccines Srl; formerly Novartis Vaccines) was presented as a lyophilized powder of the serogroup A vaccine component and a liquid serogroup C, W, and Y vaccine component in 2 separate vials which were combined for a single dose of 0.5 mL. Each dose contained 10, 5, 5, and 5 μg of serogroups A, C, W, and Y oligosaccharide, respectively, and approximately 32.7–64.1 μg of CRM197 protein carrier. Both vaccines were administered intramuscularly in the right or left deltoid region.


Blood samples for immunogenicity assessments were collected at baseline (day 0; prevaccination) and at day 30 (up to day 44) postvaccination. Functional antibody titers against meningococcal serogroups A, C, W, and Y were measured prevaccination and at day 30 postvaccination by serum bactericidal antibody assays using human complement (hSBA; Global Clinical Immunology Laboratory, Sanofi Pasteur, Swiftwater, PA) and baby rabbit complement (rSBA; Public Health England, Manchester, United Kingdom), as previously described.13,14 A subset of participants (100 participants from each age group in the 2 vaccine groups) was used to measure rSBA. The lower limit of quantification for the hSBA and rSBA assays was 1:4. Bactericidal antibody titers determined by the hSBA and rSBA assays were converted into geometric mean titers (GMTs), as described previously.15

The primary objective was to demonstrate the noninferiority of immune response following administration of a single dose of MenACYW-TT relative to MenACWY-CRM in terms of hSBA seroresponse to serogroups A, C, W, and Y at day 30. An hSBA seroresponse was defined as postvaccination titers ≥1:16 for a participant with prevaccination titers of <1:8, or at least a 4-fold increase in titers if the prevaccination titers were ≥1:8. Secondary objectives were to evaluate the hSBA GMTs at day 0 and day 30 in the total population (those 2–9 years of age) and also for the 2 age groups, 2–5 and 6–9 years. hSBA vaccine seroresponse was also assessed by age subgroups (2–5 and 6–9 years). In addition, the study described antibody titers measured by hSBA in all participants, and titers measured by rSBA in a subset of participants. Seroprotection was defined as hSBA titers ≥1:8 or rSBA titers ≥1:128.


All participants were observed for 30 minutes after vaccination to assess the occurrence of any immediate adverse events (AEs)/reactions. Participants’ parents/guardians were provided with diary cards and instructed to record information on solicited injection site (pain, erythema, and swelling) and systemic reactions (fever, headache, malaise, and myalgia) from day 0 to day 7 postvaccination (see Table, Supplemental Digital Content 1, and 2,, and unsolicited AEs through to day 30 (+14 days).

Serious AEs (SAEs) were recorded throughout the duration of the study. Parents or guardians were asked to inform the investigators of any potential SAEs immediately. AEs of special interest (AESI) monitored based on the guidance received from the European Medicines Agency included the following: Kawasaki disease; Guillain-Barré syndrome; generalized seizures, including febrile seizures; and idiopathic thrombocytopenic purpura.

Statistical Analyses

For the primary objective, the noninferiority of hSBA seroresponse with MenACYW-TT relative to MenACWY-CRM at day 30 as tested for each of the serogroups A, C, W, and Y separately. If the lower limit of the 2-sided 95% confidence interval (CI) of the difference between the 2 seroresponse rates was greater than –10%, the inferiority hypothesis was rejected. Overall noninferiority was demonstrated if all 4 individual inferiority hypotheses were rejected.

Assuming a 10% noninferiority margin, with 400 evaluable participants in each vaccine group, the study would have 90% power to declare the noninferiority of the hSBA seroresponse of MenACYW-TT to that of MenACWY-CRM. A total of 1000 participants needed to be enrolled to meet the power requirements assuming a 20% dropout rate, which would result in approximately 800 participants in the Per Protocol Analysis Set (PPAS) available for immunogenicity analyses. Power was calculated with the assumption that the seroresponse estimate from the MenACYW-TT equaled that of MenACWY-CRM.

All immunogenicity analyses were performed on the PPAS, which was composed of participants who had received the study vaccine and had at least 1 valid postvaccination serology result and met all protocol-specified inclusion criteria. Safety assessments were carried out on the Safety Analysis Set, which included all participants who received the study vaccine and had safety data available.

Categorical variables were summarized and presented as frequency counts with 95% CIs calculated using the normal approximation for quantitative data and the exact binomial distribution (Clopper-Pearson method).16 Bactericidal antibody titers and corresponding 95% CIs were calculated on Log10 transformed data assuming normal distribution for the transformed data, with antilog transformations applied to provide GMTs and their 95% CI.17


Study Participants

A total of 1000 participants were enrolled and randomized to receive either MenACYW-TT (n = 499) or MenACWY-CRM (n = 501). The participant disposition through the study is shown in Figure, Supplemental Digital Content 3 ( Of the total randomized participants, 498 (MenACYW-TT [n = 251]; MenACWY-CRM [n = 247]) were 2–5 years of age, and 502 (MenACYW-TT [n = 248]; MenACWY-CRM [n = 254]) were 6–9 years of age. All participants provided blood samples at day 0 and day 30 after vaccination. The PPAS comprised 458 and 460 participants in the MenACYW-TT and MenACWY-CRM groups, respectively. A total of 974 participants completed the study in the MenACYW-TT (n = 487) and MenACWY-CRM (n = 487) groups. Baseline demographic characteristics are summarized for the 2 groups in Table 1.

TABLE 1. - Baseline Demographics (All Randomized Participants)
(n = 499) (n = 501)
Gender, n (%)
 Male 254 (50.9) 265 (52.9)
Mean age, y (mean [SD])
 2–5 4.0 (1.2) 3.9 (1.2)
 6–9 7.9 (1.2) 8.0 (1.1)
Racial origin, n (%)
 White 402 (80.6) 417 (83.2)
 Asian 2 (0.4) 2 (0.4)
 Black/African American 66 (13.2) 61 (12.2)
 American Indian/Alaska Native 1 (0.2) 0 (0.0)
 Native Hawaiian/Pacific Islander 4 (0.8) 0 (0.0)
 Mixed origin 21 (4.2) 21 (4.2)
 Missing 3 (0.6) 0 (0.0)
Ethnicity, n (%)
 Hispanic or Latino 114 (22.8) 116 (23.2)
 Non-Hispanic or Latino 384 (77.0) 385 (76.8)
 Missing 1 (0.2) 0 (0.0)


MenACYW-TT was demonstrated to be noninferior to MenACWY-CRM in terms of hSBA seroresponse against all 4 serogroups at day 30 (Table 2). In the MenACYW-TT group, higher proportions of participants had an hSBA seroresponse with nonoverlapping 95% CIs against serogroups C, W, and Y compared with MenACWY-CRM (Table 2).

TABLE 2. - Noninferiority of the Proportion of Participants (%) Who Had Achieved hSBA Vaccine Seroresponsea at Day 30 Between Vaccine Groups (Per Protocol Analysis Set)
(n = 458) (n = 460)
Serogroup n/M % (95% CI) n/M % (95% CI) Difference, % (95% CI)
A 252/455 55.4 (50.7-60.0) 219/458 47.8 (43.2-52.5) 7.6 (1.1-14.0)
C 436/458 95.2 (92.8-97.0) 219/458 47.8 (43.2-52.5) 47.4 (42.2-52.2)
W 361/458 78.8 (74.8-82.5) 294/459 64.1 (59.5-68.4) 14.8 (8.9-20.5)
Y 419/458 91.5 (88.5-93.9) 364/459 79.3 (75.3-82.9) 12.2 (7.7-16.7)
ahSBA vaccine seroresponse was demonstrated if a participant had prevaccination titers <1:8, then the postvaccination titer had to be ≥1:16, or for a participant with a prevaccination titer ≥1:8, the postvaccination titer had to be at least 4-fold greater than the prevaccination titer; 95% CIs of the single proportion was calculated from the exact binomial method.
CI, confidence interval; PPAS, Per Protocol Analysis Set; n, number of participants who achieved an hSBA vaccine seroresponse; M, number of participants with available data for the endpoint; N, number of participants in the PPAS.
The overall noninferiority would be demonstrated if the lower limit of the 2-sided 95% CI is > –10% for all 4 serogroups.

The hSBA GMTs for all serogroups increased between day 0 and day 30 in both vaccine groups (Figure 1). At day 30, GMTs for serogroups C, W, and Y achieved with MenACYW-TT were higher than those with MenACWY-CRM, with nonoverlapping 95% CIs (Figure 1). The GMTs for serogroup A were comparable between vaccine groups (overlapping 95% CIs).

hSBA GMTs at (A) baseline (day 0) and (B) day 30 (Per Protocol Analysis Set).

The proportion of participants with hSBA titers ≥1:8 increased from baseline to day 30 for all serogroups, regardless of vaccine group (Table 3). The proportion of participants with seroprotective hSBA titers were higher in the MenACYW-TT group than the MenACWY-CRM group for serogroups C, W, and Y, with similar proportions in both groups achieving seroprotective titers against serogroup A. For rSBA titers, the proportion of participants with titers ≥1:128 was similar in both groups at day 30, except for serogroup C for which the proportion of participants was higher in the MenACYW-TT group (see Table, Supplemental Digital Content 4,

TABLE 3. - Proportion of Participants With hSBA Titers ≥1:8 at Day 0 and at Day 30 (Per Protocol Analysis Set)
Serogroups Time Point MenACYW-TT MenACWY-CRM
(n = 458) (n = 460)
n/M % (95% CI) n/M % (95% CI)
A Day 0 225/457 49.2 (44.6-53.9) 224/460 48.7 (44.0-53.4)
Day 30 394/456 86.4 (82.9-89.4) 363/458 79.3 (75.3-82.9)
C Day 0 56/458 12.2 (9.4-15.6) 59/459 12.9 (9.9-16.3)
Day 30 448/458 97.8 (96.0-98.9) 308/459 67.1 (62.6-71.4)
W Day 0 90/458 19.7 (16.1-23.6) 93/460 20.2 (16.6-24.2)
Day 30 434/458 94.8 (92.3-96.9) 396/459 86.3 (82.8-89.3)
Y Day 0 54/458 11.8 (9.0-15.1) 57/460 12.4 (9.5-15.8)
Day 30 451/458 98.5 (96.9-99.4) 417/459 90.8 (87.8-93.3)
CI, confidence interval; M, number of participants with a valid serology result for the particular serogroup and time point; n, number of participants experiencing the endpoint.
Titers ≥1:8 were considered seroprotective.

The immune response tended to be stronger in those 6–9 years than those 2–5 years of age, with similar trends observed for GMTs (see Figure, Supplemental Digital Content 5,, seroresponse rates (see Table, Supplemental Digital Content 6,, and seroprotective titers (see Figure, Supplemental Digital Content 7, For both age groups, in those who received MenACYW-TT, GMTs and seroresponse rates were highest against serogroup C; in those who received MenACWY-CRM, GMTs and seroresponse rates were highest against serogroup Y. In both age groups, the greatest proportion of participants with seroprotection (hSBA titers ≥1:8) were seen against serogroup Y in both vaccine groups.


The safety profile of MenACYW-TT was comparable to that of MenACWY-CRM. The proportion of participants reporting solicited systemic reactions and injection site reactions was similar in both vaccine groups (Table 4). No participant discontinued because of AEs or reactions. One AESI (temporal partial seizure) was reported in the MenACWY-CRM group, and it was assessed as not related to the vaccination by the Investigator. There were no SAEs considered related to vaccination in either group. When vaccine groups were stratified by age (2–5 and 6–9 years), the safety profiles were comparable.

TABLE 4. - Summary Safety Outcomes (Safety Analysis Set)
(n = 498) (n = 494)
n/M % (95% CI) n/M % (95% CI)
AEs within 30 min after vaccine injection
 Immediate unsolicited AE 0/498 0.0 (0.0-0.7) 0/494 0.0 (0.0-0.7)
 Immediate unsolicited AR 0/498 0.0 (0.0-0.7) 0/494 0.0 (0.0-0.7)
AEs within 30 d after vaccine injection
 Solicited reaction 270/487 55.4 (50.9-59.9) 296/486 60.9 (56.4-65.3)
 Solicited injection site reaction 228/487 46.8 (42.3-51.4) 262/486 53.9 (49.4-58.4)
 Pain 188/487 38.6 (34.3-43.1) 206/486 42.4 (37.9-46.9)
 Erythema 110/487 22.6 (18.9-26.6) 153/485 31.5 (27.4-35.9)
 Swelling 67/484 13.8 (10.9-17.2) 104/483 21.5 (17.9-25.5)
 Solicited systemic reaction 168/487 34.5 (30.3-38.9) 180/486 37.0 (32.7-41.5)
 Fever 9/485 1.9 (0.9-3.5) 13/479 2.7 (1.5-4.6)
 Headache 61/487 12.5 (9.7-15.8) 56/486 11.5 (8.8-14.7)
 Malaise 103/487 21.1 (17.6-25.0) 99/486 20.4 (16.9-24.2)
 Myalgia 98/487 20.1 (16.7-24.0) 112/486 23.0 (19.4-27.1)
 Unsolicited AE 121/498 24.3 (20.6-28.3) 145/494 29.4 (25.4-33.6)
 Unsolicited AR 10/498 2.0 (1.0-3.7) 17/494 3.4 (2.0-5.5)
 Related SAE 0/498 0.0 (0.0-0.7) 0/494 0.0 (0.0-0.7)
AEs during the study
 SAE 0/498 0.0 (0.0-0.7) 0/494 0.0 (0.0-0.7)
 MAAEs between visits 1 and 2 65/498 13.1 (10.2-16.3) 76/494 15.4 (12.3-18.9)
 MAAE between visit 2 to 6-month follow-up 155/498 31.1 (27.1-35.4) 136/494 27.5 (23.6-31.7)
AEs, adverse events; AR, adverse reaction; CI, confidence interval; M, number of participants with available data for the endpoint; MAAE, medically-attended AE; n, number of participants experiencing the endpoint. SAE, serious AE.


This is the first study to assess the safety and immunogenicity of MenACYW-TT compared with MenACWY-CRM in children 2–9 years of age. The noninferiority of MenACYW-TT to MenACWY-CRM in terms of the hSBA seroresponse was demonstrated for all 4 meningococcal serogroups at day 30 postvaccination. The safety profile of MenACYW-TT was similar to that of MenACWY-CRM, with no safety concerns identified.

The proportion of participants achieving an hSBA seroresponse against serogroups C, W, and Y was higher with MenACYW-TT than MenACWY-CRM, with a similar proportion of participants achieving seroresponse against serogroup A. Consistent with these observations, GMTs were higher with MenACYW-TT than MenACWY-CRM for serogroups C, W, and Y, but similar between the 2 study groups for serogroup A. hSBA GMTs against serogroup C were notably high in the MenACYW-TT group, but the clinical benefit of these high GMT levels has not yet been established. The observed differences in immune response may be due to heterogeneity in the MenACYW-TT and MenACWY-CRM vaccine formulations. While higher titers may be anticipated to lead to longer persistence of protection, we currently cannot make any predictions from the current study data. Ongoing persistence and booster studies with MenACYW-TT will be able to provide additional information.

Routine conjugate meningococcal vaccination schedules against IMD have been introduced in 21 countries based on their established efficacy, safety profile, and immunogenicity,18 with more countries in the African meningitis belt expected to follow by 2020.19 School-based immunization programs have shown to be effective in reducing cases of IMD, for example, the meningococcal C vaccine was administered in children and adolescents in the United Kingdom, leading to vaccine uptake of >85% in these age groups.20 In the present study, similar trends for protective titers and GMTs were observed for both age subgroups (2–5 and 6–9 years). There tended to be more participants who had achieved an hSBA seroresponse in the older compared with younger age group, consistent with other studies of MenACYW-TT that found a higher proportion of adults and adolescents achieved protective titers compared with children.21,22 However, this is unlikely to have any clinical significance and is an observation likely driven by physiologic differences in immune responses. Taken together, the results of the present study suggest that MenACYW-TT could be suitable for protection against IMD in either preschool or school-age children, dependent on the immunization program of a particular country.18,23

Overall, both MenACYW-TT and MenACWY-CRM were well-tolerated. The safety profiles of MenACYW-TT and MenACWY-CRM were consistent with previous studies assessing other vaccines against IMD in children.24,25 There were no AESIs or SAEs assessed as related to vaccination in either group.

The study has some limitations. While hSBA ≥1:8 or rSBA ≥1:128 titer thresholds are widely used as serologic correlates of protection for meningococcal vaccines, and have been accepted globally by health regulatory agencies,26–28 the absolute clinical benefit of these protective titers is yet to be determined.29,30 This study benefitted from enrolling a large number of study participants across 36 sites in the United States, including Puerto Rico; however, analyses comparing the populations of mainland United States and Puerto Rico have not been conducted. A high rate of adherence to the protocol among enrolled participants added to the strength of this study. High baseline titers for serogroup A were seen among the participants in this study, and another similar study in adolescents.21 Given that there is no recommendation for infant or toddler vaccination with meningococcal vaccines in the United States, the exact origin of these titers cannot be explained. Because of the potential variability of baseline titers, an advantage of using seroresponse as an endpoint for assessing immunogenicity is that it accounts for baseline seropositivity.

Our findings suggest that MenACYW-TT may have the potential to prevent IMD when administered as a single dose to meningococcal vaccine-naïve children 2–9 years of age. MenACYW-TT could also have potential in catch-up immunization programs alongside routine recommendations.


Authors wish to acknowledge all the Investigators as listed in the Supplemental Digital Content 8;, and the study site staff that were part of the study. Editorial assistance with the preparation of this manuscript was provided by Jonathon Ackroyd of inScience Communications, Springer Healthcare, and was funded by Sanofi Pasteur. Authors also wish to acknowledge and thank the Sanofi Pasteur study team especially David Neveu, Jennifer Kinsley, Lucia Perez and Amy Strickland, for the support during the conduct of this study.


1. Olbrich KJ, Müller D, Schumacher S, et al. Systematic review of invasive meningococcal disease: sequelae and quality of life impact on patients and their caregivers. Infect Dis Ther. 2018;7:421–438.
2. Acevedo R, Bai X, Borrow R, et al. The Global Meningococcal Initiative meeting on prevention of meningococcal disease worldwide: epidemiology, surveillance, hypervirulent strains, antibiotic resistance and high-risk populations. Expert Rev Vaccines. 2019;18:15–30.
3. Nadel S, Ninis N. Invasive meningococcal disease in the vaccine era. Front Pediatr. 2018;6:321.
4. European Centre for Disease Prevention and Control. 2019Factsheet about meningococcal disease. Available from: Accessed 25 October, 2019.
5. Centers for Disease Control and Prevention. 2019Meningococcal disease: technical and clinical information. Available from: Accessed 18 November, 2019.
6. Jafri RZ, Ali A, Messonnier NE, et al. Global epidemiology of invasive meningococcal disease. Popul Health Metr. 2013;11:17.
7. World Health Organization. 2018Invasive meningococcal disease—serogroup distribution, 2018. Available from: Accessed January 2020.
8. Peterson ME, Li Y, Bita A, et al.; Meningococcal Surveillance Group (in alphabetical order). Meningococcal serogroups and surveillance: a systematic review and survey. J Glob Health. 2019;9:010409.
9. Centers for Disease Control and Prevention. 2017Enhanced meningococcal disease surveillance report, 2017. Available from: Accessed 25 October, 2019.
10. Centers for Disease Control and Prevention. 2019Epidemiology and prevention of vaccine-preventable diseases: meningococcal disease. Available from:
11. Shaker R, Fayad D, Dbaibo G. Challenges and opportunities for meningococcal vaccination in the developing world. Hum Vaccin Immunother. 2018;14:1084–1097.
12. 2016World Health Organization. Meningitis A nearly eliminated in Africa through vaccination, reaching more than 235 million people. Available from: Accessed 25 November, 2019.
13. Maslanka SE, Gheesling LL, Libutti DE, et al. Standardization and a multilaboratory comparison of Neisseria meningitidis serogroup A and C serum bactericidal assays. The Multilaboratory Study Group. Clin Diagn Lab Immunol. 1997;4:156–167.
14. Pina LM, Bassily E, Machmer A, et al. Safety and immunogenicity of a quadrivalent meningococcal polysaccharide diphtheria toxoid conjugate vaccine in infants and toddlers: three multicenter phase III studies. Pediatr Infect Dis J. 2012;31:1173–1183.
15. Mak PA, Santos GF, Masterman KA, et al. Development of an automated, high-throughput bactericidal assay that measures cellular respiration as a survival readout for Neisseria meningitidis. Clin Vaccine Immunol. 2011;18:1252–1260.
16. Erdoğan S, Gülhan OT. Alternative confidence interval methods used in the diagnostic accuracy studies. Comput Math Methods Med. 2016;2016:7141050.
17. Zhou XH, Gao S. Confidence intervals for the log-normal mean. Stat Med. 1997;16:783–790.
18. Ali A, Jafri RZ, Messonnier N, et al. Global practices of meningococcal vaccine use and impact on invasive disease. Pathog Glob Health. 2014;108:11–20.
19. Bwaka A, Bita A, Lingani C, et al. Status of the rollout of the Meningococcal Serogroup A conjugate vaccine in African meningitis belt countries in 2018. J Infect Dis. 2019;220(220 suppl 4):S140–S147.
20. Vuocolo S, Balmer P, Gruber WC, et al. Vaccination strategies for the prevention of meningococcal disease. Hum Vaccin Immunother. 2018;14:1203–1215.
21. Chang LJ, Hedrick J, Christensen S, et al. A phase II, randomized, immunogenicity and safety study of a quadrivalent meningococcal conjugate vaccine, MenACYW-TT, in healthy adolescents in the United States. Vaccine. 2020;38:3560–3569.
22. Dhingra MS, Peterson J, Hedrick J, et al. Immunogenicity, safety and inter-lot consistency of a meningococcal conjugate vaccine (MenACYW-TT) in adolescents and adults: a phase III randomized study. Vaccine. 2020;38:5194–5201.
23. Perman S, Turner S, Ramsay AI, et al. School-based vaccination programmes: a systematic review of the evidence on organisation and delivery in high income countries. BMC Public Health. 2017;17:252.
24. Knuf M, Romain O, Kindler K, et al. Immunogenicity and safety of the quadrivalent meningococcal serogroups A, C, W-135 and Y tetanus toxoid conjugate vaccine (MenACWY-TT) in 2-10-year-old children: results of an open, randomised, controlled study. Eur J Pediatr. 2013;172:601–612.
25. Halperin SA, Baine Y, Domachowske JB, et al. Comparison of the safety and immunogenicity of a novel quadrivalent meningococcal ACWY-tetanus toxoid conjugate vaccine and a marketed quadrivalent meningococcal ACWY-diphtheria toxoid conjugate vaccine in healthy individuals 10-25 years of age. J Pediatric Infect Dis Soc. 2014;3:33–42.
26. Andrews N, Borrow R, Miller E. Validation of serological correlate of protection for meningococcal C conjugate vaccine by using efficacy estimates from postlicensure surveillance in England. Clin Diagn Lab Immunol. 2003;10:780–786.
27. Rivero-Calle I, Raguindin PF, Gómez-Rial J, et al. Meningococcal group B vaccine for the prevention of invasive meningococcal disease caused by Neisseria meningitidis serogroup B. Infect Drug Resist. 2019;12:3169–3188.
28. World Health Organization. Meningococcal vaccines: WHO position paper, November 2011. Wkly Epidemiol Rec. 2011;86:521–539.
29. Gill CJ, Ram S, Welsch JA, et al. Correlation between serum bactericidal activity against Neisseria meningitidis serogroups A, C, W-135 and Y measured using human versus rabbit serum as the complement source. Vaccine. 2011;30:29–34.
30. Findlow J, Balmer P, Borrow R. A review of complement sources used in serum bactericidal assays for evaluating immune responses to meningococcal ACWY conjugate vaccines. Hum Vaccin Immunother. 2019;15:2491–2500.

children; conjugate vaccine; invasive meningococcal disease; MenACWY-CRM; MenACYW-TT; meningococcal vaccination; noninferiority

Supplemental Digital Content

Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc.