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Species-specific PCR Improves Detection of Bacterial Pathogens in Parapneumonic Empyema Compared With 16S PCR and Culture

Blaschke, Anne J. MD, PhD; Byington, Carrie L. MD; Ampofo, Krow MD; Pavia, Andrew T. MD; Heyrend, Caroline BSc; Rankin, Shelley C. PhD; McGowan, Karin L. PhD; Harris, Mary C. MD; Shah, Samir S. MD, MSCE

The Pediatric Infectious Disease Journal: March 2013 - Volume 32 - Issue 3 - p 302–303
doi: 10.1097/INF.0b013e3182784ec1
Letters to the Editor

Department of PediatricsUniversity of Utah

Department of PediatricsUniversity of UtahBioFire Diagnostics, Inc. (formerly Idaho Technology, Inc.)Salt Lake City, UT

Department of MicrobiologyUniversity of Pennsylvania School of Veterinary Medicine

Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of Medicine

Department of PediatricsUniversity of Pennsylvania School of MedicinePhiladelphia, PA

Divisions of Hospital Medicine and Infectious DiseasesCincinnati Children’s Hospital Medical CenterCincinnati, OH

This work was supported by the National Institute of Allergy and Infectious Diseases at the National Institutes of Health (NIH; 1K23AI079401 to AJB, K01AI73729 to SSS, 1U01AI082184-01 to AJB and ATP, 1R01 AI089489-01 to ATP); the National Institute of Child Health and Development at the NIH (K24HD047249-01 to CLB); the Centers for Disease Control (1U18IP000303-01 to AJB, KKA, CLB, ATP); the McCabe Fund Advisory Committee (to SSS), and the University Research Foundation of the University of Pennsylvania (to SSS). The project described was supported by the National Center for Research Resources and the National Center for Advancing Translational Sciences, NIH (UL1RR025764 to CLB and AJB, UL1RR024134 to SSS). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. CH was an employee of Idaho Technology, Inc. (now BioFire Diagnostics, Inc.) at the time of the study. AJB, CLB, ATP and KA collaborate with BioFire Diagnostics, Inc., on several NIH and Centers for Disease Control and Prevention-funded projects. AJB and CLB have intellectual property in BioFire Diagnostics, Inc. The authors have no other funding or conflicts of interest to disclose.

AJB and CH contributed equally to the work.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s website (

The authors have no funding or conflicts of interest to disclose.

© 2013 Lippincott Williams & Wilkins, Inc.