Translational Medicine ReportsUse of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric EndocarditisTo, Rachel K. MS*; Ramchandar, Nanda MD, MPH†,‡; Gupta, Aamisha MD†,‡; Pong, Alice MD†,‡; Cannavino, Christopher MD†,‡; Foley, Jennifer BSN‡; Farnaes, Lauge MD, PhD‡,§; Coufal, Nicole G. MD, PhD†,§Author Information From the *Chicago Medical School, Rosalind Franklin University of Medicine and Science, Chicago, IL †Department of Pediatrics, University of California, San Diego, CA ‡Rady Children’s Hospital of San Diego, San Diego, CA §Rady Children’s Institute for Genomic Medicine, San Diego, CA. Accepted for publication December 5, 2020 The authors have no funding or conflicts of interest to disclose. Address for correspondence: Nicole G. Coufal, MD, PhD, University of California, San Diego, 9500 Gilman Drive MC 0695, La Jolla, CA 92093. E-mail: [email protected]. The Pediatric Infectious Disease Journal: May 2021 - Volume 40 - Issue 5 - p 486-488 doi: 10.1097/INF.0000000000003038 Buy Metrics Abstract Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.