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Pathogenic Bacterial Nasopharyngeal Colonization and Its Impact on Respiratory Diseases in the First Year of Life: The PATCH Birth Cohort Study

Tsai, Ming-Han MD, PhD*†; Huang, Shu-Huan MS; Chen, Chyi-Liang PhD†§; Chiu, Chih-Yung MD, PhD*†; Hua, Man-Chin MD*†; Liao, Sui-Ling MD*†; Yao, Tsung-Chieh MD, PhD†¶; Lai, Shen-Hao MD†‖; Yeh, Kuo-Wei MD†¶; Wang, Mei-Ping BS*; Huang, Jing-Long MD†¶

The Pediatric Infectious Disease Journal: June 2015 - Volume 34 - Issue 6 - p 652–658
doi: 10.1097/INF.0000000000000688
Pathogenesis and Host Response
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Background: For acute respiratory diseases caused by bacteria, colonization in the respiratory tracts is often the first sign, although nasopharynx is the major source of secretions containing pathogens. To understand the pathogenesis of respiratory tract diseases, it is important to analyze the establishment of nasopharyngeal bacterial colonization.

Methods: Infants with nasopharyngeal swabs were examined at the age of 1, 2, 4, 6 and 12 months for the detection of pathogens, including Streptococcus pneumoniae, Hemophilus influenzae, Moraxella catarrhalis, Streptococcus pyogenes and Staphylococcus aureus. The methods used for detection were bacterial culture and multiplex polymerase chain reaction.

Results: From January 2012 to August 2013, a total of 320 neonates were enrolled, and 120 of them completed the first 12-month study. Staphylococcus aureus was the most common pathogen at all 5 time points while the rates declined; in contrast, the other 4 increased during the first year of life. Of our series, the multiplex polymerase chain reaction detection rates were higher than those of bacterial culture. More than 50% of Staphylococcus aureus was methicillin-resistant, and the trend decreased in the same period. In the analysis of factors associated with the development of infant wheeze, infants with maternal atopy [odds ratio (OR): 3.26; 95% confidence interval (CI): 1.20–8.88; P = 0.02] and pneumococcal colonization (OR: 15.64; 95% CI: 3.25–75.35; P = 0.001) had higher rates of wheeze.

Conclusions: Bacterial interactions may result in differing pathogen prevalence in the first year of life. In addition, nasopharyngeal pneumococcal colonization may have an effect on the risk of infant wheeze. The result could help clinicians to clarify the relation between bacterial colonization and respiratory illnesses in infancy.

From the *Department of Pediatrics, Chang Gung Memorial Hospital, Keelung, Taiwan; Chang Gung University, College of Medicine, Taoyuan, Taiwan; Department of Medical Laboratory, Chang Gung Memorial Hospital, Keelung, Taiwan; §Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Division of Allergy, Asthma, and Rheumatology, and Division of Pulmonology, Department of Pediatrics, Chang Gung Children’s Hospital, Taoyuan, Taiwan.

Accepted for publication October 20, 2014.

The study was supported by grants CMRPG 2B0031-3 from Chang Gung Memorial Hospital, Keelung, Taiwan. The authors have no conflicts of interest to declare.

Address for correspondence: Jing-Hong Huang, MD, Department of Pediatrics, Chang Gung Children’s Hospital, No. 5 Fu-Hsin Street, Kweishan 333, Taoyuan, Taiwan. E-mail: long@adm.cgmh.org.tw

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