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Prevalence and Molecular Epidemiology of Noroviruses in Hospitalized Children With Acute Gastroenteritis in Rio de Janeiro, Brazil, 2004

Victoria, Matías MSc; Carvalho-Costa, Filipe Aníbal MSc; Heinemann, Marcos Bryan PhD; Leite, José Paulo PhD; Miagostovich, Marize PhD

The Pediatric Infectious Disease Journal: July 2007 - Volume 26 - Issue 7 - p 602-606
doi: 10.1097/INF.0b013e3180618bea
Original Studies

Background: The role of noroviruses (NoV) as a cause of gastroenteritis outbreaks is well documented; however, the importance of NoV infections in hospitalized children is not well established. The aim of this study was to determine the prevalence and the genetic diversity of NoV in hospitalized children.

Methods: Three-hundred eighteen fecal samples were collected from January to December 2004, from children with acute gastroenteritis in 3 public hospitals in Rio de Janeiro, Brazil. The prevalence and genetic diversity of NoV was carried out by using genome amplification and sequencing of polymerase and capsid genes.

Results: NoV infections were detected in 65 (20%) of the samples, of which 11 (4%) were mixed infections with rotavirus. Infants up to 1-year-old were the most affected and a peak of virus detection was observed in autumn and spring seasons. Dehydration and diarrhea were the inclusion criterion; coughing (51%), vomiting (33%), and fever (22%) were the main clinical manifestations. Phylogenetic analysis showed that Genogroup II and GII/4 were prevalent. Two potential recombinant strains based in the different clustering pattern were observed.

Conclusions: This study demonstrated the importance of NoV infections causing severe acute gastroenteritis in hospitalized children in Rio de Janeiro, Brazil. Molecular epidemiology surveillance determining the circulation pattern of different genotypes and recombinant strains is helpful for designing prevention strategies of NoV transmission in children. Studies concerning the prevalence and the molecular epidemiology of gastroenteric viruses in hospitalized children are particularly important to evaluate the impact of the rotavirus vaccine in Brazil.

Supplemental Digital Content is Available in the Text.

From the Laboratory of Comparative Virology, Department of Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

Accepted for publication March 22, 2007.

Address for correspondence: Matías Victoria, Laboratório de Virologia Comparada, Departamento de Virologia, Pavilhão Cardoso Fontes, Av. Brasil 4.365, Manguinhos, CEP 21040-000, Rio de Janeiro, RJ, Brasil. E-mail:

© 2007 Lippincott Williams & Wilkins, Inc.