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Abstract PD-020: SERUM MIR-126-3P LEVEL IS DOWN-REGULATED IN SEPSIS PATIENTS

Wen, T.1

Pediatric Critical Care Medicine: June 2018 - Volume 19 - Issue 6S - p 35
doi: 10.1097/01.pcc.0000537422.51536.55
Poster Discussion Abstracts
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1The First Affiliated Hospital of Sun Yat-Sen University, Department of Pediatric intensive care unit, Guang zhou, China

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Aims & Objectives:

Background:Endothelial injury is one of pathogenesis of sepsis. The microRNA-126 (miR-126) was previously identified as an endothelial biomarker and is known to play a critical role in preserving endothelial cell integrity. However, the role of miRNA-126 in sepsis is unclear

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Methods

Blood samples were collected from sepsis patients at the first Affiliated Hospital of Sun Yat-sen University within 24 h (n = 60) and on day 7 after (n = 51) diagnosis, and once from control subjects (n = 46). MiR-126-3p expression was evaluated by quantitative real-time PCR. The miR-126-3p level was correlated with clinical data and a set of routine and experimental biomarkers. The outcome of sepsis patients was determined by a follow-up at 28 days after collection of blood samples on day 7.

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Results

MiR-126-3p level was significantly down-regulated in sepsis patients 24 h after diagnosis compare with control subjects. And the more serious sepsis was, the lower level of serum miR-126-3p was. To determine the diagnostic accuracy of miR-126-3p, the receiver operating characteristic (ROC) was performed and the AUC of miR-126-3p was 0.735. Furthermore, serum miR-126-3p concentration at this time point was correlated with the expression markers of systemic inflammation, bacterial infection, and renal and hepatic dysfunction. However, serum miR-126-3p level on day 7 day did not differ between surviving sepsis patients and those who died.

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Conclusions

These results indicate that miR-126-3p could be a diagnostic biomarker for sepsis patients.

©2018The Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies