To examine whether the septic profiles of heat shock protein 72, heat shock protein 90α, resistin, adiponectin, oxygen consumption, CO2 production, energy expenditure, and metabolic pattern, along with illness severity, nutritional, and inflammatory indices, differ between adult and pediatric patients compared with systemic inflammatory response syndrome and healthy controls. To evaluate whether these biomolecules may discriminate sepsis from systemic inflammatory response syndrome in adult and pediatric patients.
Prospective cohort study.
University ICU and PICU.
Seventy-eight adults (sepsis/23; systemic inflammatory response syndrome/23; healthy controls/33), 67 children (sepsis/18; systemic inflammatory response syndrome/23; controls/27), mechanically ventilated.
Flow cytometry determined mean fluorescence intensity for monocyte or neutrophil heat shock protein expression. Resistin, adiponectin, and extracellular heat shock proteins were measured using enzyme-linked immunosorbent assay; energy expenditure by E-COVX (GE Healthcare). Genomic DNA was extracted with PureLink Genomic DNA kit (Invitrogen, Carlsbad, CA) to detect heat shock protein 72 single nucleotide polymorphisms. Similarly, in adult and pediatric patients, Acute Physiology and Chronic Evaluation-II/Acute Physiology and Pediatric Risk of Mortality-III, Simplified Acute Physiology Score-III, C-reactive protein, lactate, and resistin were higher and myocardial contractility, monocyte heat shock protein 72, oxygen consumption, CO2 production, energy expenditure, metabolic pattern, glucose, and albumin lower in sepsis compared with systemic inflammatory response syndrome or controls (p < 0.05). For discriminating sepsis from systemic inflammatory response syndrome, resistin, extracellular heat shock protein 90α, and lactate achieved a receiver operating characteristic curve greater than 0.80 in children and greater than 0.75 in adults (p < 0.05). In both, adults and children, genotype heat shock protein 72 analysis did not disclose any diagnosis or mortality group differences regarding either rs6457452 or rs1061581 haplotypes.
Sepsis presents with similar profiles in adult and pediatric patients, characterized by enhanced inflammatory hormonal response and by repressed innate immunity, metabolism, and myocardial contractility. These features early distinguish sepsis from systemic inflammatory response syndrome across all age groups.
1University of Crete, Medical School, University Hospital, PICU, Heraklion, Greece.
2University of Crete, Medical School, Paediatric Haematology Oncology, Heraklion, Greece.
3University of Crete, Medical School, University Hospital, ICU, Heraklion, Greece.
4Institute of Molecular Biology and Biotechnology (IMBB), Postgenomic Research Laboratory, Foundation for Research and Technology - Hellas (FORTH), Heraklion, Greece.
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Drs. Tavladaki and Spanaki contributed equally. Drs. Tavladaki and Spanaki have generated, collected, and assembled the data; Dr. Choulaki substantially contributed to the analysis of samples; Dr. Briassoulis conceived and designed the study; Drs. Dimitriou, Kondili, and Georgopoulos substantially contributed to the collection of data (either clinical or laboratory), interpretation of them and revision and editing of the article; Drs. Tavladaki, Spanaki, and Kondili organized patient recruitment; Drs. Tavladaki and Briassoulis analyzed and interpreted the data and wrote the article; and all authors edited and approved the final version of the article.
The authors have disclosed that they do not have any potential conflicts of interest.
This research has been cofinanced by the European Union (European Social Fund) and Greek national funds through the Operational Program ‘‘Education and Lifelong Learning’’ of the National Strategic Reference Framework-Research Funding Program: THALES. The sponsor had no active role in the design, methods, data collections, analysis, or preparation of this article or the decision to submit the article for publication.
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