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Cardiac Dysfunction in Pneumovirus-Induced Lung Injury in Mice

Bem, Reinout A. MD, PhD1; van den Berg, Elske MD1; Suidgeest, Ernst MSc2; van der Weerd, Louise MD, PhD2,3; van Woensel, Job B. M. MD, PhD1; Grotenhuis, Heynric B. MD, PhD2,4

Pediatric Critical Care Medicine: June 2013 - Volume 14 - Issue 5 - p e243–e249
doi: 10.1097/PCC.0b013e31828a7f9b
Online Laboratory Investigations

Objective: To determine biventricular cardiac function in pneumovirus-induced acute lung injury in spontaneously breathing mice.

Design: Experimental animal study.

Setting: Animal laboratory.

Subjects: C57Bl/6 mice.

Intervention: Mice were inoculated with the rodent pneumovirus, pneumonia virus of mice.

Measurements and Main Results: Pneumonia virus of mice-infected mice were studied for right and left ventricular function variables by high-field strength (7 Tesla) cardiac MRI at specific time points during the course of disease compared with baseline. One day before and at peak disease severity, pneumonia virus of mice-infected mice showed significant right and left ventricular systolic and diastolic volume changes, with a progressive decrease in stroke volume and ejection fraction. No evidence for viral myocarditis or viral presence in heart tissue was found.

Conclusions: These findings show adverse pulmonary-cardiac interaction in pneumovirus-induced acute lung injury, unrelated to direct virus-mediated effects on the heart.

1Pediatric Intensive Care Unit, Emma Children’s Hospital AMC, Amsterdam, The Netherlands.

2Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands.

3Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands.

4Department of Pediatric Cardiology, Leiden University Medical Center, Leiden, The Netherlands.

Supported, in part, by the Gratama Foundation and Leiden University Fund.

The authors have disclosed that they do not have any potential conflicts of interest.

Address requests for reprints to: R. A. Bem, MD, PhD, Pediatric Intensive Care Unit, Emma Children’s Hospital Academic Medical Center, PO Box 22660, 1100 DD Amsterdam, The Netherlands. E-mail:

©2013The Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies